Eppendorf Research Plus Single-Channel Adjustable-Volume Pipette, 0.1–2.5 µL
| Brand | Eppendorf |
|---|---|
| Origin | Germany |
| Type | Manual Single-Channel Pipette |
| Volume Range | 0.1–2.5 µL |
| Maximum Capacity | 2.5 µL |
| Accuracy | ±0.03 µL |
| Compliance | ISO 8655–2 (volumetric performance), GLP/GMP-ready design |
Overview
The Eppendorf Research Plus Single-Channel Adjustable-Volume Pipette (0.1–2.5 µL) is a precision-engineered manual liquid handling instrument designed for high-accuracy dispensing in molecular biology, genomics, and analytical chemistry workflows. Built upon Eppendorf’s patented spring-loaded piston mechanism and low-force pipetting technology, this model delivers consistent volumetric performance across its full range — from ultra-low volumes (0.1 µL) to 2.5 µL — with minimal user-induced variability. Its air displacement principle ensures compatibility with aqueous, mildly viscous, and volatile liquids while maintaining traceable metrological integrity per ISO 8655–2. The pipette is calibrated at factory conditions (20 °C, 50% RH) and certified for use in regulated environments requiring documentation of measurement uncertainty and traceability to national standards.
Key Features
- Ultra-low-volume capability: Optimized for critical applications such as qPCR master mix preparation, CRISPR reagent dilution, and single-cell lysate handling where sub-microliter accuracy is non-negotiable.
- Ergonomic design: Lightweight polymer housing with adjustable tip ejection force and soft-touch volume adjustment dial reduces repetitive strain injury (RSI) risk during extended use.
- Chemical resistance: Corrosion-resistant internal components and UV-stable polycarbonate body ensure longevity when exposed to ethanol, DMSO, and common laboratory solvents.
- Calibration-ready: Integrated calibration port allows field adjustment using standard gravimetric or photometric methods; certificate of conformity includes uncertainty budget per ISO/IEC 17025 guidelines.
- Tip compatibility: Designed exclusively for Eppendorf epT.I.P.S.® low-retention, filter, and sterile tips — ensuring minimal carryover and optimal seal integrity at low volumes.
Sample Compatibility & Compliance
This pipette supports reproducible handling of DNA/RNA solutions, enzyme cocktails, fluorescent dyes, and diluted protein standards without measurable adsorption or evaporation artifacts. It complies with ISO 8655–2 for volumetric accuracy and repeatability, and its mechanical construction meets IEC 61000–4 electromagnetic compatibility requirements. For laboratories operating under Good Laboratory Practice (GLP) or Good Manufacturing Practice (GMP), the Research Plus platform supports audit-ready documentation through optional calibration logs and traceable serial-number registration. While not inherently compliant with FDA 21 CFR Part 11, it integrates seamlessly with validated LIMS and ELN systems when used with Eppendorf’s electronic calibration management software.
Software & Data Management
Although the 0.1–2.5 µL Research Plus is a manual device, its operational data can be captured via Eppendorf’s free LabManager™ desktop application (Windows/macOS). Users may log calibration dates, technician IDs, environmental conditions, and verification results — generating PDF reports compliant with ISO/IEC 17025 Annex A.2 requirements. When paired with Eppendorf’s SmartCheck™ pipette verification system (sold separately), real-time performance diagnostics — including aspiration/dispense consistency, tip-seal integrity, and piston friction analysis — are recorded and exportable in CSV or XML format for quality assurance review.
Applications
- Quantitative nucleic acid dilution for digital PCR and NGS library quantification
- Precision dispensing of restriction enzymes and ligases in cloning workflows
- Preparation of calibration standards for HPLC and mass spectrometry
- Low-volume cell culture media supplementation and transfection reagent delivery
- Microfluidic chip priming and assay plate spotting in HTS environments
FAQ
Is this pipette suitable for handling viscous or volatile liquids?
Yes — its optimized piston travel path and temperature-compensated spring system minimize evaporation and backflow errors. However, for highly viscous samples (>100 mPa·s), pre-wetting and reverse pipetting techniques are recommended.
What calibration frequency is recommended for ISO 8655 compliance?
Annual calibration is standard; however, daily verification using distilled water gravimetry is advised for critical applications. Laboratories under CAP or CLIA accreditation may require quarterly inter-laboratory verification.
Can this pipette be sterilized in an autoclave?
No — the Research Plus series is not autoclavable. Sterilization is achieved only through chemical disinfection (e.g., 70% ethanol wipe) or use of pre-sterilized epT.I.P.S.® tips.
Does Eppendorf provide a certificate of calibration with purchase?
Each unit ships with a factory-issued Certificate of Conformity referencing ISO 8655–2. A full ISO/IEC 17025-accredited calibration certificate is available upon request at additional cost.
How does the 0.1–2.5 µL range compare to other Research Plus models in terms of mechanical wear?
The ultra-low-range variant employs a reinforced micro-piston assembly and tighter tolerance machining to preserve long-term accuracy — resulting in a service life exceeding 100,000 cycles under proper maintenance protocols.
