Thermo Scientific Multiskan SkyHigh UV-Vis Full-Spectrum Microplate Reader
| Brand | Thermo Scientific |
|---|---|
| Origin | Singapore |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | Multiskan SkyHigh |
| Function Type | Multi-Mode (Endpoint, Kinetic, Spectrum Scan, Kinetic Spectrum Scan) |
| Automation Level | Fully Automated Microplate Reader |
| Detection Method | Absorbance (UV-Vis) |
| Wavelength Range | 200–1000 nm |
| Absorbance Range | 0–4 OD |
| Linearity | ±2% from 0–2.5 Abs |
| Wavelength Scanning Speed | 6 s per 96-well plate |
| Precision | SD < 0.003 Abs |
Overview
The Thermo Scientific Multiskan SkyHigh is a high-performance, fully automated UV-Vis microplate reader engineered for precision absorbance measurements across the full spectral range of 200–1000 nm. Based on robust photometric principles—employing a high-stability xenon flash lamp and double-grating monochromator—the instrument delivers exceptional wavelength accuracy, repeatability, and low stray light performance essential for demanding life science applications. Designed for laboratories requiring regulatory compliance and reproducible quantitative analysis, the SkyHigh supports endpoint, kinetic, and full-spectrum scanning assays—including simultaneous kinetic spectrum acquisition—enabling comprehensive characterization of biomolecular interactions, enzyme kinetics, and nucleic acid/protein quantification without hardware reconfiguration.
Key Features
- Fully integrated touchscreen interface with intuitive icon-driven navigation and multilingual support (English, Chinese, Japanese, German, French, Spanish)
- High-speed spectral scanning: complete 96-well plate measurement in ≤6 seconds at user-defined wavelengths or full-range scan (200–1000 nm) with 1 nm resolution
- Optimized optical path design delivering <0.003 Abs standard deviation across 0–2.5 Abs range, validated per ISO 15197 and CLSI EP6-A guidelines
- Integrated μDrop Duo microvolume plate supporting dual-mode operation: 2–4 µL nucleic acid/protein quantitation (A260/A280) plus dedicated cuvette port for standard 1 cm pathlength measurements
- Automated plate handling with barcode reader option for GLP-compliant sample tracking and audit trail generation
- Compliance-ready architecture supporting 21 CFR Part 11 requirements via SkanIt software’s electronic signature, role-based access control, and immutable audit logs
Sample Compatibility & Compliance
The Multiskan SkyHigh accommodates all standard microplate formats (6–384-well), including opaque, clear, and UV-transparent plates. Its μDrop Duo module enables direct quantification of un-diluted DNA, RNA, and protein samples (e.g., BSA, IgG) with no carryover between measurements—validated per ISO/IEC 17025 calibration protocols. The system meets key international standards for clinical and research instrumentation: ASTM E275 for spectrophotometer performance verification, ISO 8655-7 for pipette-assisted microvolume validation, and USP for UV-Vis absorbance system suitability testing. Optional IQ/OQ documentation packages are available to support GMP/GLP facility qualification.
Software & Data Management
SkanIt PC software provides a unified platform for method development, instrument control, real-time data visualization, and compliant reporting. It features drag-and-drop workflow builder, built-in curve-fitting algorithms (linear, quadratic, 4-/5-parameter logistic, log-log), and automated concentration calculation using user-defined extinction coefficients or reference standards. All data files include embedded metadata (wavelength, integration time, plate map, operator ID) and export to CSV, PDF, or XML formats compatible with LIMS and ELN systems. When paired with Thermo Fisher Connect cloud services, users gain secure remote monitoring, encrypted data synchronization across devices, automatic firmware updates, and centralized instrument fleet management—all accessible via web browser or Instrument Connect mobile app (iOS/Android).
Applications
- Nucleic acid quantification (dsDNA, ssDNA, RNA) and purity assessment (A260/A280, A260/A230 ratios)
- Protein concentration determination via Bradford, BCA, Lowry, or direct A280 measurement
- Enzyme activity assays (e.g., LDH, ALP, β-galactosidase) using kinetic or endpoint detection
- Cell viability and cytotoxicity studies (MTT, XTT, Resazurin)
- ELISA and immunoassay development with multi-wavelength correction and background subtraction
- Chemical screening and small-molecule binding kinetics in 96-/384-well formats
FAQ
Does the Multiskan SkyHigh support FDA 21 CFR Part 11 compliance?
Yes—when used with SkanIt software v5.2 or later and configured with electronic signatures, audit trail logging, and user access controls, the system satisfies core Part 11 requirements for electronic records and signatures.
Can I perform both microvolume and cuvette measurements on the same instrument?
Yes—the μDrop Duo module includes a dedicated horizontal cuvette slot alongside the microvolume pedestal, enabling seamless switching between 2–4 µL sample analysis and standard 1 cm pathlength measurements.
Is wavelength calibration traceable to NIST standards?
Yes—factory calibration is performed using NIST-traceable holmium oxide and didymium filters; optional annual recalibration services include full wavelength accuracy and photometric linearity certification.
What data security protocols are implemented in Thermo Fisher Connect?
All data transmissions use TLS 1.2+ encryption; cloud storage complies with ISO 27001 and SOC 2 Type II frameworks; user authentication supports SAML 2.0 and multi-factor authentication (MFA).
Can the instrument be integrated into an automated liquid handling workflow?
Yes—it supports RS-232, USB, and Ethernet interfaces with ASCII command protocol for bidirectional communication with robotic arms, stackers, and third-party LIMS platforms.
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