HUXI HW-200TG Dual-Plate Microplate Incubator Shaker

Overview

The HUXI HW-200TG Dual-Plate Microplate Incubator Shaker is an integrated laboratory instrument engineered for precise temperature-controlled orbital shaking of microplates and cell culture plates. Combining PID-based intelligent temperature regulation with uniform film heating technology, the HW-200TG delivers stable thermal environments and reproducible agitation across two independent plate positions simultaneously. Its design follows fundamental principles of convective heat transfer and controlled mechanical oscillation—enabling consistent kinetic energy input during enzymatic assays, cell-based screening, ELISA development, bacterial growth studies, and nucleic acid hybridization protocols. Unlike generic shakers, this system integrates real-time thermal feedback control at the plate interface level, minimizing thermal gradients and ensuring assay fidelity under dynamic conditions.

Key Features

• Dual-plate configuration supports parallel processing of two standard microplates (96-well, 384-well) or T-flasks/cell culture dishes—increasing throughput without compromising control.
• PID-controlled heating system with dual-zone film heaters ensures rapid thermal equilibration (≤15 min from 25°C to 70°C) and maintains temperature stability within ±0.3°C, with uniformity across plate surfaces better than ±0.5°C.
• Independent adjustment of temperature, orbital speed (100–1600 rpm), and timing enables method-specific optimization—critical for sensitive applications such as protein expression, ligand-binding kinetics, and metabolic activity profiling.
• LCD interface displays real-time setpoints and operational status—including remaining time, current temperature, and actual rpm—supporting GLP-compliant documentation practices.
• Hardware- and software-based overtemperature protection mechanisms meet CE safety directives (2014/35/EU Low Voltage Directive and 2014/30/EU EMC Directive), ensuring fail-safe operation in shared lab environments.
• Auto-restart function resumes pre-interruption settings after power loss—minimizing experimental variability and supporting unattended overnight runs.

Sample Compatibility & Compliance

The HW-200TG accommodates ANSI/SBS-standard microplates (including deep-well, PCR, and assay plates) and common tissue culture formats (e.g., 6-, 12-, 24-, 48-, and 96-well plates; T25/T75 flasks). Its low-profile platform geometry prevents plate displacement during high-speed shaking. The unit complies with IEC 61010-1:2010 for laboratory electrical equipment safety and is CE-marked for use in EU-regulated research and quality control laboratories. While not certified for GMP manufacturing environments, its deterministic timer logic, non-volatile parameter memory, and audit-ready operational logging support internal SOPs aligned with ISO/IEC 17025 and FDA 21 CFR Part 11 data integrity expectations when paired with validated lab information management systems (LIMS).

Software & Data Management

The HW-200TG operates via embedded firmware with no external PC dependency. All parameters—including temperature setpoint, speed, duration, and auto-restart enablement—are stored in non-volatile memory. The LCD provides immediate visual verification of active conditions, eliminating reliance on proprietary software or drivers. For integration into automated workflows, optional RS-232 or USB-to-serial interfaces (available upon request) allow remote command execution and event logging via third-party control platforms. Timestamped run logs—including start/stop times, final temperature deviation, and error codes—can be exported for traceability in regulated environments.

Applications

• Enzyme-linked immunosorbent assay (ELISA) incubation and washing steps requiring simultaneous agitation and thermal control.
• Cell-based assays involving transient transfection, reporter gene expression, or cytokine stimulation under defined thermal and mixing conditions.
• Molecular biology workflows including DNA/RNA hybridization, restriction digestion, and ligase reactions where reaction kinetics depend on both temperature and homogenization.
• Microbial growth profiling across temperature gradients in multi-plate comparative studies.
• High-throughput screening (HTS) support where reproducible plate-level agitation minimizes edge effects and improves signal-to-noise ratios.
• Antibody-antigen binding kinetics studies requiring precise dwell time and mixing consistency across replicates.

FAQ

Does the HW-200TG support programmable ramping or gradient temperature profiles?
No. It maintains a single user-defined setpoint throughout the run; multi-step thermal protocols require manual reconfiguration between cycles.
Can the unit operate continuously beyond 99 hours?
Yes—selecting the “∞” timer mode enables uninterrupted operation until manually stopped.
Is calibration certificate included with shipment?
Factory calibration is performed per internal QA protocol; NIST-traceable calibration documentation is available as an optional add-on service.
What maintenance is required for long-term reliability?
Routine cleaning of the plate platform and ventilation grilles is recommended; no scheduled lubrication or sensor recalibration is necessary under normal usage conditions.
Is the device compatible with CO₂ incubators or anaerobic chambers?
No—it is designed for ambient-air operation only and lacks gas-tight sealing or humidity control features.