Boxun BSD-WX(F)1350 Ultra-Large-Capacity Horizontal Incubator Shaker

Overview

The Boxun BSD-WX(F)1350 is an ultra-large-capacity horizontal incubator shaker engineered for high-throughput, temperature-sensitive biological culture and biochemical reaction workflows. Designed with dual-mode oscillation—orbital and reciprocating—it supports a broad spectrum of life science applications requiring precise environmental control and mechanical agitation. Its working chamber (1035 × 675 × 470 mm) accommodates up to 15 × 2 L flasks or 7 × 5 L vessels on a single stainless-steel shelf, enabling scalable cultivation of microbial, mammalian, and insect cell lines under tightly regulated thermal and mechanical conditions. The system employs PID-controlled forced-air convection for uniform temperature distribution (±1 °C at 25 °C, empty chamber) and maintains stability within ±0.2 °C at 37 °C—critical for reproducible fermentation, enzyme kinetics, and hybridization protocols. Unlike compact benchtop units, the BSD-WX(F)1350 integrates structural rigidity, low-noise operation (<55 dB(A)), and industrial-grade thermal management—including optimized defrost logic that minimizes temperature excursion during cycle maintenance—making it suitable for GMP-aligned labs and long-duration unattended runs.

Key Features

• PID microprocessor controller with backlit LCD display showing real-time setpoints and measured values for temperature, speed, amplitude, and remaining time
• Programmable multi-segment curve control (up to 10 segments), supporting complex ramp-hold-cool profiles for adaptive bioprocess development
• Dual oscillation modes: orbital (30–300 rpm, Φ26 mm amplitude) and reciprocating (30–250 rpm, 0–50 mm linear stroke), each with ±1 rpm speed accuracy
• Brushless DC motor drive ensuring constant torque across full speed range, zero carbon wear, and stable low-speed performance (no drift below 50 rpm)
• Imported precision bearings and reinforced chassis delivering >50,000 h operational lifetime and acoustic noise reduction
• Triple safety architecture: independent overtemperature cutoff, door interlock switch, and motor thermal protection with automatic power cutoff upon fault detection
• Power-fail recovery function: resumes pre-interruption settings and runtime upon AC restoration without manual reset
• Soft-start acceleration algorithm prevents sample splashing and ensures gentle initiation of motion, preserving fragile cultures and agar-based media
• Electrostatically coated steel exterior, mirror-finish 304 stainless steel interior, and rounded chamber corners for ISO-compliant cleaning and decontamination
• Optional inert gas inlet port (N₂/CO₂) for anaerobic or controlled-atmosphere applications; optional RS485 interface for LabVIEW or SCADA integration

Sample Compatibility & Compliance

The BSD-WX(F)1350 supports standard microbiological and bioprocessing vessel formats including Erlenmeyer flasks (50–5000 mL), baffled bottles, and custom bioreactor bags mounted on universal clamps. Its large deck (970 × 605 mm for orbital / 950 × 635 mm for reciprocating) allows flexible configuration of bottle holders, tube racks, or microplate adapters. The unit complies with IEC 61010-1:2010 for laboratory equipment safety and meets electromagnetic compatibility requirements per EN 61326-1. While not certified for Class II biosafety containment, its sealed chamber design and optional UV sterilization accessory support GLP-compliant documentation practices. Temperature and speed logging—when paired with optional printer or PC software—enables audit-ready records aligned with FDA 21 CFR Part 11 data integrity expectations.

Software & Data Management

The embedded controller stores up to 10 user-defined programs with timestamped execution logs. Optional PC software (BoxunShake Control Suite v3.x) provides remote monitoring, real-time graphing of temperature/speed profiles, and export of CSV-formatted datasets for statistical analysis in JMP or Python. An optional thermal printer generates hard-copy reports with date/time stamps, operator ID, and deviation alerts. For networked environments, GPRS-enabled alarm modules send SMS notifications upon threshold breach (e.g., T > 60.5 °C or speed variance > ±3 rpm for >60 s). All digital outputs include metadata fields required for ISO/IEC 17025 traceability frameworks.

Applications

• High-density bacterial and yeast fermentation (E. coli, Pichia pastoris, Bacillus subtilis) under controlled O₂ transfer conditions
• Mammalian suspension cell expansion in serum-free media for monoclonal antibody production
• Enzymatic digestion, DNA ligation, and PCR master mix equilibration requiring uniform thermal homogeneity
• Environmental microbiology studies involving soil slurry enrichment or wastewater biofilm development
• Pharmaceutical stability testing per ICH Q1A(R2) guidelines using temperature-cycled shaking protocols
• Food safety validation assays (e.g., pathogen enrichment in buffered peptone water per AOAC 998.12)
• Plant tissue culture agitation during callus induction and secondary metabolite extraction

FAQ

What is the maximum flask size supported on a single shelf?
The orbital configuration accommodates up to fifteen 2 L Erlenmeyer flasks or seven 5 L vessels; reciprocating mode supports identical volumetric capacity with enhanced shear sensitivity for shear-tolerant cultures.
Does the unit support humidity control?
Yes—the BSD-WX(F)1350 maintains 40–95% RH with ±5% fluctuation, enabled by integrated steam generator and condensate management system (standard on F-series models).
Can the shaker be validated for IQ/OQ/PQ protocols?
Full validation documentation—including factory calibration certificates, sensor traceability to NIST standards, and FAT/SAT test scripts—is available upon request for regulated pharmaceutical or clinical lab deployment.
Is remote access possible without third-party hardware?
Native Ethernet connectivity is not built-in, but the RS485 port supports Modbus RTU communication for integration into existing BMS or PLC networks via external protocol converters.
What maintenance intervals are recommended?
Brushless motor requires no scheduled maintenance; annual verification of temperature uniformity (per ASTM E145 Annex A3) and speed calibration (using optical tachometer) is advised for GLP/GMP compliance.