Yoke UV752N Ultraviolet-Visible Spectrophotometer

Overview

The Yoke UV752N Ultraviolet-Visible Spectrophotometer is a manually operated, single-beam optical instrument engineered for routine quantitative and qualitative analysis in academic teaching laboratories, quality control environments, and small-scale R&D settings. It operates on the fundamental principle of Beer–Lambert law-based absorbance measurement, utilizing a Czerny-Turner monochromator equipped with a 1200 lines/mm holographic grating to resolve spectral energy across a broad 195–1020 nm range—covering deep UV through near-infrared. Its single-beam architecture, while requiring manual wavelength adjustment via calibrated mechanical dial, delivers robust baseline stability and high photometric reproducibility under controlled ambient conditions. Designed for reliability over extended service life, the UV752N integrates automatic shutter control to minimize detector exposure during idle periods—a feature critical for preserving photodiode array longevity and maintaining long-term signal fidelity.

Key Features

• Single-beam optical path with Czerny-Turner monochromator and 1200 L/mm grating for consistent spectral resolution
• Wide sample compartment accommodating standard cuvettes from 5 mm to 100 mm pathlength, supporting both microvolume and macrovolume analysis
• Dual-lamp system with imported deuterium (UV) and tungsten (VIS-NIR) sources, automatically switched at ~340 nm to ensure optimal spectral continuity and intensity
• Photodiode array detector providing stable, low-noise response with ≤0.2% T noise level and ±0.002 A/h baseline drift at 500 nm
• Manual wavelength selection interface offering tactile precision and operational transparency—ideal for pedagogical use and method development where parameter visibility is essential
• Integrated automatic light shutter to protect detector integrity and extend service intervals without user intervention
• 4-digit LED display with dual-mode readout: transmittance (0–200% T) and absorbance (–0.301 to 2.000 A), supporting concentration calculation via built-in C-mode functionality

Sample Compatibility & Compliance

The UV752N accommodates standard rectangular quartz, glass, or plastic cuvettes (10 × 10 mm cross-section) with pathlengths ranging from 5 mm to 100 mm. It supports aqueous, organic, and mildly corrosive solutions when used with appropriate cell materials. While not certified for GLP/GMP-regulated production environments, its photometric accuracy (±0.5% T) and wavelength fidelity (±2 nm) meet typical requirements for educational validation, raw material screening, and in-process checks aligned with ASTM E275 and ISO 6059 standards. The instrument’s mechanical construction and lamp management comply with IEC 61010-1 safety specifications for laboratory electrical equipment. No embedded software audit trail or electronic signature capability is provided; thus, it is not compliant with FDA 21 CFR Part 11 for regulated data capture.

Software & Data Management

The UV752N operates as a standalone hardware platform with no internal microprocessor-based data logging or USB connectivity. Quantitative output—including absorbance, transmittance, and concentration values—is displayed in real time on the front-panel LED. Optional parallel-port thermal printer integration enables hard-copy documentation of single-point or multi-wavelength measurements. Users must perform calibration verification (e.g., NIST-traceable holmium oxide or potassium dichromate filters) manually prior to each analytical session. For laboratories requiring digital archiving, external PC-based acquisition via analog voltage output (0–1 V corresponding to 0–2 A) is feasible using third-party DAQ systems—though this requires custom configuration and is outside the scope of factory support.

Applications

• Educational instruction in spectroscopic principles, calibration curve construction, and spectrophotometric assay development
• Quantitative determination of nucleic acids (A₂₆₀) and proteins (A₂₈₀) in biological extracts
• Colorimetric assays including phosphate, nitrate, iron, and COD analysis per APHA Standard Methods
• Quality assessment of dyes, pigments, and pharmaceutical excipients in non-GMP manufacturing support roles
• Stability testing of UV-absorbing compounds under accelerated light exposure protocols
• Verification of filter cutoff characteristics and optical material transmission profiles

FAQ

Is the UV752N suitable for regulatory-compliant QC in pharmaceutical manufacturing?
No. It lacks electronic audit trail, user access controls, and 21 CFR Part 11–compliant data handling features required for GMP environments.
Can the instrument measure absorbance beyond 2.000 A?
No. Its photometric range is limited to –0.301 to 2.000 A. Samples exceeding 2.000 A require dilution or reduced pathlength to remain within linear dynamic range.
What maintenance is required for long-term wavelength accuracy?
Annual verification using NIST-traceable reference filters (e.g., holmium oxide at 241.1, 279.4, 360.9 nm) is recommended. Grating alignment is factory-set and not field-adjustable.
Does the UV752N support kinetic or scanning modes?
No. It is a fixed-wavelength or step-scan instrument only; continuous spectral scanning is not supported.
Is firmware upgrade capability available?
No. The instrument contains no programmable memory or firmware; all functionality is implemented in discrete analog/digital circuitry.