Sanotac ZB-100 Preparative High-Performance Liquid Chromatography System
| Brand | Sanotac |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Domestic |
| Model | ZB-100 |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative LC |
| Flow Rate Range | 0.01–100.00 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ≤0.5% |
| Maximum Pressure | 20 MPa |
| Wavelength Range | 190–800 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Reproducibility | 0.2 nm |
| Baseline Noise | ±0.75×10⁻⁵ AU (254 nm, TC = 1 s) |
| Data Acquisition Rate | 10 Hz |
Overview
The Sanotac ZB-100 Preparative High-Performance Liquid Chromatography (Prep-HPLC) System is an engineered solution for the isolation and purification of reference standards in natural product chemistry and traditional Chinese medicine (TCM) research. Designed for laboratory-scale preparative separations, it operates on the principle of high-pressure liquid chromatography with gradient elution, enabling reproducible fractionation of complex botanical extracts under controlled isocratic or multi-step gradient conditions. The system integrates a dual-piston reciprocating pump architecture with real-time pressure feedback compensation, minimizing solvent compressibility effects and delivering stable flow across its full dynamic range (0.01–100.00 mL/min). Its UV-Vis detector supports dual-wavelength monitoring and time-programmable wavelength scanning—critical for resolving co-eluting compounds such as saponins, flavonoids, alkaloids, and anthraquinones commonly found in TCM matrices. Built to meet rigorous analytical traceability requirements, the ZB-100 conforms to regulatory frameworks governing data integrity in regulated environments.
Key Features
- Microprocessor-controlled dual-piston pump with cavity pressure feedback for precise flow delivery and minimized pulsation
- Flow pulse suppression via cam-profile compensation technology, achieving baseline noise as low as ±0.75×10⁻⁵ AU at 254 nm (1 s time constant)
- Multi-point flow calibration across the entire operational range ensures ±1% flow accuracy and ≤0.5% RSD repeatability
- Floating plunger design extends seal lifetime under sustained 20 MPa operation
- 10 programmable user methods supporting gradient slope modification, flow ramping, and hold steps during runtime
- Dual-lamp source (deuterium + tungsten) with automatic lamp switching and hot-swap flow cell capability for maximum instrument uptime
- UV-Vis detector with 190–800 nm spectral coverage, ±1 nm wavelength accuracy, and 0.2 nm reproducibility
- Automated fraction collector featuring hybrid linear-rotary motion for rapid positioning and sub-millisecond valve actuation
- Configurable collection modes: time-based, peak-triggered, threshold-level, slope-detection, sequential, and cyclic collection
- Integrated delay volume compensation algorithm to align retention time with physical fraction collection—minimizing cross-contamination
Sample Compatibility & Compliance
The ZB-100 accommodates standard preparative columns (e.g., C18, silica, NH₂, diol) from major vendors including GE Healthcare (AKTA), Biotage, ISCO, Buchi, and Bio-Rad—enabling seamless method transfer and column interchangeability without hardware adaptation. It supports both solid-phase loading (via 10 mL loop valve) and direct liquid injection, making it suitable for crude extract purification, impurity isolation, and reference material synthesis. The system complies with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) documentation expectations. Its Sanochrom software meets CFDA GxP and U.S. FDA 21 CFR Part 11 requirements, providing electronic signatures, audit trails, user access controls, and immutable data archiving—ensuring full traceability for regulatory submissions related to herbal monographs, pharmacopoeial standards, and chemical impurity qualification.
Software & Data Management
Controlled via Sanochrom workstation software running on Windows 7/8/10 platforms, the ZB-100 offers a graphical interface accessible through USB or RS-232 connectivity. The software enables synchronized control of pump gradients, UV detection parameters, and fraction collector logic—including real-time multi-wavelength signal overlay and dynamic threshold adjustment. All acquisition events are timestamped and logged with operator ID, method name, and instrument state metadata. Audit trail records capture every parameter change, method execution, and data export action. Raw chromatograms and collected fractions are stored in vendor-neutral formats (e.g., .cdf, .csv) compatible with third-party analysis tools. Software validation packages—including IQ/OQ documentation templates—are available upon request to support lab qualification processes.
Applications
The ZB-100 is routinely deployed in academic, pharmaceutical, and contract research laboratories for the preparative isolation of phytochemical reference substances. Validated applications include:
- Purification of triterpenoid saponins from Astragalus membranaceus and Ginseng extracts
- Isolation of flavonoid glycosides (e.g., rutin, hesperidin) and aglycones from citrus and leguminous sources
- Separation of isoflavones (daidzein, genistein) and coumarins (scopoletin, imperatorin) from herbal matrices
- Recovery of alkaloids (berberine, matrine) and phenolic acids (rosmarinic, chlorogenic) under pH-stable mobile phases
- Resolution of terpenoids, anthraquinones (emodin, rhein), and lignans (schisandrin, arctigenin) using reversed-phase and normal-phase protocols
- Impurity profiling and isolation of synthetic drug degradation products per ICH Q3 guidelines
FAQ
What column formats and dimensions is the ZB-100 compatible with?
The system supports standard preparative columns ranging from 10 mm to 50 mm internal diameter, including stainless steel and PEEK-bodied units. It accepts cartridges and packed columns from AKTA, Biotage, ISCO, and other industry-standard suppliers.
Does the system support method transfer from analytical HPLC to preparative scale?
Yes—the ZB-100 allows retention time scaling, flow rate normalization, and gradient translation using built-in scaling algorithms; users can import analytical methods and adjust parameters proportionally for load capacity and resolution optimization.
Can the UV detector perform spectral scanning during peak collection?
Yes—time-programmed wavelength scanning and stop-flow spectral acquisition are supported, enabling peak purity assessment and offline library matching without interrupting the run.
Is remote monitoring or network integration possible?
While the base configuration uses local PC control, optional Ethernet-enabled modules allow integration into LAN-based lab networks for centralized instrument status monitoring and scheduled batch runs.
What validation documentation is provided with the system?
Sanotac supplies factory test reports, installation qualification (IQ) checklists, and operational qualification (OQ) protocols aligned with ISO/IEC 17025 and ASTM E2500 standards; full 21 CFR Part 11 validation support packages are available under separate service agreement.
