Cole-Parmer MFLA74900-05 Single-Channel Precision Syringe Pump
| Brand | Avantor |
|---|---|
| Origin | USA |
| Model | MFLA74900-05 |
| Flow Rate | 0.2 µL/h to 60 mL/h |
| Accuracy | ±0.5% |
| Repeatability | ±0.2% |
| Syringe Capacity | 10 µL–60 mL |
| Linear Force | 20 lbf (9.1 kgf) |
| Power Supply | 230 VAC, 50/60 Hz |
| Warranty | 1 year |
Overview
The Cole-Parmer MFLA74900-05 is a single-channel, microprocessor-controlled syringe pump engineered for high-precision, low-flow-rate fluid delivery in analytical, pharmaceutical, and life science laboratories. It operates on the principle of linear displacement via a stepper motor-driven lead screw mechanism, translating rotational motion into precise axial movement of the syringe plunger. This architecture ensures stable, pulseless flow across an exceptionally wide dynamic range—up to 6000:1—enabling accurate delivery from nanoliter-per-hour infusions to milliliter-per-hour bolus injections. Designed specifically for applications requiring minimal shear stress and pressure sensitivity—such as microfluidic device priming, electrophysiology perfusion, HPLC sample introduction, and controlled reagent dosing—the pump maintains consistent volumetric accuracy without reliance on external feedback sensors. Its closed-loop control logic, combined with automatic syringe barrel recognition, eliminates manual unit conversion errors and supports traceable, repeatable operation under GLP-compliant workflows.
Key Features
- Microprocessor-based control with auto-syringe identification: detects syringe diameter and dynamically scales flow rate display and calibration to µL/h, mL/h, or mL/min—enabling resolution down to 0.2 µL/h.
- Backlit alphanumeric LCD interface with intuitive menu navigation for real-time parameter configuration, including flow rate, volume, direction (infuse/withdraw), and ramp profiles.
- Non-volatile memory retains all user-defined settings—including target volume, flow rate, and operating mode—even after power cycling, ensuring protocol continuity and audit readiness.
- High mechanical reproducibility (±0.2%) and volumetric accuracy (±0.5%) validated per ISO 8536-4 and ASTM D3418 standards for syringe pump performance testing.
- Robust linear actuation system delivering 20 lbf (9.1 kgf) of push/pull force, compatible with glass, plastic, and stainless-steel syringes ranging from 10 µL to 60 mL capacity.
- Front-panel programmability supports real-time parameter adjustment during active infusion, allowing adaptive experimental control without interrupting flow.
Sample Compatibility & Compliance
The MFLA74900-05 accommodates standard Luer-lock and Luer-slip syringes from major manufacturers (e.g., BD, Hamilton, SGE), including low-dead-volume and gas-tight variants. It complies with IEC 61010-1:2010 for electrical safety in laboratory equipment and meets EMC requirements per EN 61326-1:2013. While not certified for sterile or in vivo use, its design supports integration into ISO 13485-aligned quality systems when deployed in QC/QA environments. All operational parameters—including start time, elapsed volume, and flow deviation logs—are timestamped and exportable for 21 CFR Part 11–compliant data integrity when connected to validated software platforms.
Software & Data Management
The pump features an RS-232 serial interface (DB9 male) for bidirectional communication with host computers running terminal emulation or custom LabVIEW/Python scripts. ASCII command protocol enables remote initiation, pause/resume, direction reversal, and real-time status polling (e.g., “STATUS?”, “VOLUME?”, “FLOW?”). Optional Cole-Parmer PumpControl™ software provides graphical workflow sequencing, multi-pump synchronization, and CSV-formatted audit trails with operator ID tagging—essential for FDA-regulated documentation. All firmware updates are delivered via secure HTTPS download and applied through the serial interface, preserving version control and change history per GMP Annex 11 guidelines.
Applications
- Chromatography: Precise mobile-phase or internal-standard delivery in UHPLC and capillary electrophoresis systems.
- Neuroscience: Continuous drug infusion into brain tissue or cell cultures with sub-nanoliter precision over extended durations.
- Materials science: Controlled deposition of polymer solutions or nanoparticle suspensions in thin-film fabrication.
- In vitro diagnostics: Calibrator and control reagent dispensing in automated immunoassay platforms.
- Method development: Gradient flow profiling for viscosity or reaction kinetics studies where temporal resolution exceeds peristaltic pump capabilities.
FAQ
What syringe sizes are supported?
Syringes from 10 µL to 60 mL volume capacity are fully compatible, provided they conform to ISO 8536-1 dimensional tolerances and feature standard Luer interfaces.
Can this pump withdraw fluid as well as infuse?
Yes—the MFLA74900-05 supports bidirectional operation (infuse/withdraw) with identical accuracy and repeatability specifications in both directions.
Is the flow rate adjustable during operation?
Yes—flow rate, volume, and direction can be modified in real time via front-panel controls or RS-232 commands without stopping the pump.
Does it meet regulatory requirements for pharmaceutical manufacturing?
While the pump itself is not GMP-certified, its deterministic behavior, non-volatile memory, and RS-232 audit trail capability enable qualification under user-defined SOPs aligned with USP , ASTM E2500, and EU GMP Annex 15.
What is the maximum allowable backpressure?
The pump is rated for continuous operation up to 60 psi (4.1 bar); sustained pressures above this threshold may affect long-term mechanical stability and require external pressure relief integration.
