MKN SH120 Microhematocrit Centrifuge

Overview

The MKN SH120 Microhematocrit Centrifuge is a compact, high-speed benchtop centrifuge engineered specifically for rapid and precise separation of capillary blood samples in clinical, point-of-care, and research laboratory settings. It operates on the principle of sedimentation under controlled centrifugal force, enabling accurate hematocrit (Hct) determination and plasma isolation from whole blood volumes as low as 10–20 µL. Unlike general-purpose centrifuges, the SH120 employs a fixed-angle rotor optimized for capillary tube geometry, ensuring uniform radial acceleration and reproducible pellet formation. Its design complies with fundamental requirements for hematology instrumentation defined in CLSI H26-A3 (Clinical Laboratory Standards Institute) and supports standardized microhematocrit methodology per ISO 15189:2022 Annex B. The unit is intended for intermittent use only—continuous operation is prohibited to maintain thermal stability and bearing longevity.

Key Features

• Fixed-angle rotor accommodating 24 capillary tubes (1.5 mm diameter × 75 mm length), arranged symmetrically for balanced load distribution and vibration minimization.
• Continuously variable speed control (0–12,000 rpm) with digital display, allowing fine-tuned optimization for different anticoagulants (e.g., EDTA, heparin) and hematocrit ranges.
• High RCF output of 13,500 × g at maximum speed ensures complete red blood cell packing within ≤5 minutes for standard capillary fill volumes.
• Integrated safety interlock system that prevents operation unless the lid is fully closed and latched—meets IEC 61010-1:2010 Class II requirements for laboratory electrical equipment.
• Low thermal mass aluminum rotor and brushless DC motor enable rapid acceleration (<60 s) and deceleration (<120 s), reducing turnaround time between runs without compromising rotor integrity.
• Timer with audible end-of-run alert (0–30 min range); automatic shutdown upon completion eliminates risk of over-centrifugation or tube fracture.

Sample Compatibility & Compliance

The SH120 accepts standard microhematocrit capillaries—including heparinized, EDTA-coated, and plain glass or plastic tubes—without adapter modification. Its rotor geometry maintains consistent orientation during spin, minimizing meniscus distortion and facilitating accurate post-centrifugation reading using calibrated hematocrit readers or digital imaging systems. While not certified for diagnostic use under FDA 510(k) or CE-IVD directives, the device meets general laboratory equipment performance criteria outlined in ISO/IEC 17025:2017 for test method validation support. It is suitable for QC verification in GLP-compliant environments when used with documented SOPs and routine calibration checks (e.g., tachometer verification, timer accuracy assessment).

Software & Data Management

The SH120 is a standalone electromechanical instrument with no embedded software or data logging capability. All operational parameters are set manually via front-panel controls; no USB, Ethernet, or Bluetooth connectivity is provided. This architecture ensures electromagnetic compatibility in sensitive lab environments and eliminates cybersecurity concerns associated with networked instruments. For laboratories requiring audit trails or electronic records, integration with external documentation systems (e.g., LIMS or ELN) must be performed manually—users record run parameters (speed, time, date, operator ID) in logbooks compliant with 21 CFR Part 11 guidelines where applicable. Optional third-party timer-integrated barcode scanners may be deployed to link centrifuge runs with sample IDs during high-throughput workflows.

Applications

• Routine hematocrit measurement in veterinary clinics, mobile health units, and resource-limited laboratories.
• Plasma isolation for subsequent analysis (e.g., glucose, electrolytes, coagulation assays) using capillary-derived specimens.
• Quality control of blood collection tubes—verifying anticoagulant efficacy and clotting inhibition.
• Training and education in clinical laboratory science programs, emphasizing hands-on understanding of sedimentation kinetics and RCF calculation.
• Supporting pre-analytical phase validation in ISO 15189-accredited labs, particularly for capillary-based POCT platforms.

FAQ

Is the SH120 suitable for continuous operation?
No. The device is rated for intermittent use only, with a maximum runtime of 30 minutes per cycle and mandatory cooldown intervals between consecutive runs to prevent motor overheating and bearing wear.

Can I use non-standard capillary tubes?
Only tubes conforming to outer diameter 1.5 mm and length 75 mm are mechanically compatible. Deviations may cause imbalance, rotor damage, or inconsistent sedimentation profiles.

Does the SH120 require periodic calibration?
Yes. Users should verify rotational speed accuracy annually using a certified handheld tachometer and confirm timer deviation ≤ ±1% per CLSI EP09-A3 protocol.

What maintenance is required?
Routine cleaning of the rotor chamber with 70% ethanol; visual inspection of rotor grooves for cracks or corrosion before each use; biannual lubrication of drive shaft bearings by authorized service personnel.

Is the unit compliant with international electrical safety standards?
Yes—it conforms to IEC 61010-1:2010 for laboratory electrical equipment, including protection against electric shock, mechanical hazards, and abnormal temperature rise.