HePu Master-JR 2021 CO₂ Incubator
| Brand | HePu (Jihepu) |
|---|---|
| Origin | Shandong, China |
| Manufacturer Type | Authorized Distributor |
| Model | Master-JR 2021 CO₂ |
| Temperature Range | +5°C to 60°C |
| Temperature Uniformity | ±0.3°C at 37°C |
| Temperature Accuracy | ±0.1°C |
| CO₂ Range | 0.1–20% |
| CO₂ Control Accuracy | ±0.3% |
| CO₂ Stability | ±0.1% |
| CO₂ Sensor Type | Infrared (IR) |
| Chamber Capacity | 170 L |
| Internal Dimensions (W×D×H) | 470 × 514 × 609 mm |
| External Dimensions (W×D×H) | 620 × 794 × 780 mm |
| Net Weight | 100 kg |
| Heating Power | 900 W |
| Display | 4.3-inch True-Color Touchscreen |
| Humidity Control | Optional Integrated Stainless Steel Heating Plate System |
| Sterilization Options | 140°C Dry-Heat, UV Lamp, or Steam-Based Moist Heat (Configurable) |
| Data Logging | Built-in USB Export |
| Remote Monitoring | Optional Wi-Fi/Cloud Interface |
| Compliance | Designed for GLP/GMP environments with audit trail, electronic signature, and role-based access control (Level 3 User Permissions) |
Overview
The HePu Master-JR 2021 CO₂ Incubator is a precision-engineered cell culture environment system designed for reproducible, contamination-minimized growth of mammalian, insect, and stem cell lines under tightly controlled physiological conditions. It operates on the principle of dual-loop feedback regulation: an independent infrared (IR) CO₂ sensor continuously monitors gas concentration in real time, while a high-stability platinum resistance thermometer (PT100) and PID-controlled heating system maintain thermal setpoints across the entire chamber volume. Unlike thermal conductivity (TC) sensors, IR detection ensures long-term drift-free performance—even during frequent door openings—and eliminates cross-sensitivity to humidity or temperature fluctuations. The incubator’s active airflow architecture integrates six-sided heating elements with a low-turbulence internal fan, enabling rapid recovery (<15 min to re-stabilize at 37°C/5% CO₂ after 30-second door opening) and uniform temperature distribution (±0.3°C at 37°C), critical for sensitive primary cultures and co-culture experiments.
Key Features
- True-color 4.3-inch touchscreen interface with intuitive multilingual (English/Chinese) navigation, real-time trend visualization, and multi-segment programmable profiles.
- Triple-layer safety architecture: personnel protection (door interlock, overtemperature cutoff), sample integrity (CO₂/temperature deviation alarms with audible/visual alerts), and equipment durability (fan failure detection, heater redundancy monitoring).
- Optional integrated humidity control module featuring a 304 stainless steel heated reservoir and closed-loop PID regulation—capable of maintaining >95% RH at 37°C without condensation or water evaporation artifacts.
- 140°C dry-heat sterilization cycle validated per ISO 14644-1 Class 5 cleanroom compatibility; eliminates need for disassembly or autoclaving of internal components.
- GMP-compliant firmware supporting 21 CFR Part 11 requirements: electronic signatures, immutable audit trails, password-protected tiered access (Administrator / Operator / Viewer), and timestamped event logging.
- Modular design allows stacking up to two units vertically; footprint-optimized for benchtop or floor-standing installation in regulated laboratories.
Sample Compatibility & Compliance
The Master-JR 2021 accommodates standard T-flasks (up to T225), multi-well plates (6–96-well), Petri dishes, roller bottles, and bioreactor vessels via configurable stainless steel shelving (4 or 8 shelves optional). Its 304 stainless steel interior—featuring seamless mirror-finish corners and radius-rounded edges—ensures easy decontamination and minimizes microbial retention points. All gas inlet pathways include dual-stage filtration (0.2 µm particulate + activated carbon), reducing VOC and endotoxin ingress from external CO₂ supply lines. The system complies with ISO 13485:2016 quality management standards for medical device manufacturing environments and supports validation protocols aligned with ASTM E2500-13 (Good Practice for Verification and Validation of Pharmaceutical Equipment).
Software & Data Management
Onboard firmware records all operational parameters—including temperature, CO₂ concentration, humidity (if equipped), door status, alarm events, and sterilization cycles—with timestamps accurate to ±1 second. Data is stored internally for ≥30 days and exportable via USB 2.0 to CSV or PDF formats compatible with LIMS integration. Optional remote monitoring enables secure web-based access through TLS-encrypted connections, allowing lab managers to review live metrics, receive SMS/email alerts for deviations, and generate compliance-ready reports. All data exports retain cryptographic hash verification to satisfy FDA audit requirements for data integrity and traceability.
Applications
This incubator serves as a core platform in academic research labs, biopharmaceutical process development, contract testing organizations (CTOs), and clinical diagnostics facilities. Typical use cases include: maintenance of induced pluripotent stem cells (iPSCs) requiring strict pH stability via 5% CO₂ buffering; hybridoma culture for monoclonal antibody production; 3D spheroid formation assays where oxygen gradient control is augmented by precise CO₂ modulation; and microbiological work involving fastidious pathogens (e.g., Helicobacter pylori) demanding stable microaerophilic conditions. Its GMP-ready architecture also supports QC release testing in accordance with USP and EP 2.6.27 guidelines.
FAQ
Does the Master-JR 2021 support 21 CFR Part 11 compliance?
Yes—firmware includes electronic signature capability, role-based permissions (three-tier access), and tamper-evident audit logs with user ID and timestamp stamping.
What is the recovery time after door opening?
Less than 15 minutes to restore both temperature (±0.3°C) and CO₂ concentration (±0.1%) at 37°C/5% setpoint following a 30-second door exposure.
Can humidity be monitored and controlled without optional hardware?
No—humidity sensing and regulation require installation of the dedicated stainless steel heating plate module and capacitive RH sensor.
Is UV sterilization integrated or external?
A built-in 254 nm UV-C lamp is standard; intensity and duration are programmable and logged as part of the sterilization protocol.
What power supply specifications are required?
Standard operation uses 220 VAC ±10%, 50 Hz single-phase input; maximum current draw is 4.1 A at full heating load.
Are calibration certificates available for sensors?
Factory-calibrated IR CO₂ and PT100 sensors ship with NIST-traceable calibration reports; field recalibration tools and procedures are provided in the technical manual.
