PreFluid WL300B High-Flow Spring-Loaded Peristaltic Pump

Overview

The PreFluid WL300B is a high-performance, spring-loaded peristaltic pump engineered for precision fluid handling in biopharmaceutical, laboratory, and process-scale applications. Unlike conventional roller-based peristaltic mechanisms, the WL300B employs a patented spring-loaded dual-rotor head design—specifically the TH25 and TH16 variants—that dynamically modulates compression force across the tubing circumference. This mechanical principle minimizes shear stress on sensitive biological fluids (e.g., mammalian cell suspensions, viral vectors, monoclonal antibodies), preserving viability and structural integrity during transfer. The pump operates on the fundamental principle of occlusion-based positive displacement: rotating spring-biased rollers compress flexible tubing against a rigid housing, creating a sealed peristaltic wave that advances fluid unidirectionally without contact between the drive mechanism and the process stream. Its robust metal chassis, electropolished surface finish, and antimicrobial coating comply with pharmaceutical cleanroom requirements—including frequent CIP/SIP cycles—making it suitable for GMP-aligned environments.

Key Features

• Spring-loaded dual-rotor pump head (TH25 or TH16) delivering ultra-low shear operation—critical for shear-sensitive biologics including CHO cell cultures and lentiviral particles.
• Wide speed control range: 0.1–300 rpm with <0.1 rpm resolution, enabling precise flow rate modulation from µL/min to 3.2 L/min (TH25) or 3.0 L/min (TH16).
• Integrated 4.3-inch backlit LCD with bilingual (English/Chinese) menu navigation, real-time display of speed, flow rate, run time, totalized volume, and system status.
• Advanced programmable functions: timed dispensing, volumetric dosing, cumulative volume tracking, and power-loss memory retention—automatically restores prior operational parameters after interruption.
• Brushless DC motor drive ensuring silent operation (20,000 hours MTBF).
• IP65-rated aluminum alloy enclosure with medical-grade antimicrobial coating—compatible with ethanol, hydrogen peroxide vapor, and saturated steam sterilization protocols.

Sample Compatibility & Compliance

The WL300B accommodates standard peristaltic tubing sizes 15#, 24#, 35#, and 36# (Pharmed® BPT, Norprene®, Santoprene®), supporting inner diameters from 0.8 mm to 6.4 mm. Its TH25 head maintains linear flow-pressure response up to 1.5 bar—validated per ASTM D3417 for dynamic viscosity stability under pressure—making it suitable for tangential flow filtration (TFF), virus filtration (VF), and HPLC mobile phase delivery where pulse-free, low-shear flow is mandatory. The system meets ISO 9001:2000 quality management standards; its firmware architecture supports audit-ready event logging (optional GLP/GMP mode), and hardware design aligns with IEC 61000-6-2 EMC immunity requirements. While not FDA 21 CFR Part 11–certified out-of-the-box, the pump’s deterministic control logic and non-volatile parameter storage facilitate validation documentation for regulated bioprocess workflows.

Software & Data Management

The WL300B operates autonomously via front-panel controls but integrates seamlessly with external SCADA or PLC systems through RS-485 Modbus RTU protocol (standard) and optional analog 0–10 V / 4–20 mA I/O. All operational events—including start/stop commands, speed changes, fault triggers (e.g., tubing rupture detection via torque anomaly), and power interruptions—are timestamped and stored in internal flash memory (10,000-event capacity). Exportable CSV logs support traceability for batch records. Optional PC software (PreFluid PumpControl Suite v3.2) enables remote configuration, multi-pump synchronization, and export of calibrated flow curves—essential for process validation under Annex 1 and USP analytical instrument qualification guidelines.

Applications

• Downstream bioprocessing: buffer exchange, diafiltration feed, harvest clarification, and chromatography column loading in mAb and vaccine manufacturing.
• Cell therapy workflows: gentle perfusion of bioreactors, media exchange in rocking platforms, and closed-system filling of cryobags.
• Quality control labs: automated sample introduction for HPLC/UHPLC systems, dissolution testing apparatus interfacing, and viscosity-standard calibration loops.
• Industrial fermentation: pH-adjustment acid/base dosing, antifoam addition, and continuous bleed-and-feed strategies requiring long-term reliability (24/7 duty cycle validated).
• Academic research: microfluidic interface priming, organ-on-chip perfusion, and synthetic biology reactor feeding where pulsation-free, contamination-free transfer is non-negotiable.

FAQ

What tubing sizes are compatible with the WL300B TH25 pump head?
The TH25 head supports tubing sizes #15, #24, #35, and #36—covering inner diameters from 0.8 mm to 6.4 mm—and is optimized for Pharmed BPT and Norprene formulations.
Does the WL300B support regulatory-compliant data integrity practices?
Yes—its embedded event logger provides tamper-resistant timestamps and parameter history. When configured with optional audit-trail firmware, it satisfies ALCOA+ principles for electronic records in GxP environments.
Can the pump maintain consistent flow under variable backpressure?
Within its rated 1.5 bar limit, the spring-loaded rotor design ensures <±2% flow deviation across pressure gradients—verified per ISO 8573-1 Class 4 purity benchmarks for compressed air-assisted calibration.
Is remote monitoring possible without additional hardware?
Yes—RS-485 Modbus RTU is built-in; no gateway or adapter is required to integrate with DeltaV, Siemens Desigo, or LabVIEW-based supervisory systems.
What is the expected service life of the brushless DC motor?
Rated for >20,000 hours of continuous operation at nominal load, with thermal protection and current-limiting circuitry preventing demagnetization or winding degradation.