Shanghai Youyi LHS-250CB Low-Temperature Temperature and Humidity Controlled Chamber

Overview

The Shanghai Youyi LHS-250CB is an upright, air-cooled temperature and humidity controlled chamber engineered for precise, long-term environmental simulation in regulated laboratory and quality assurance settings. It operates on the principle of active thermoelectric and vapor-phase humidification control, integrating a dual-loop microprocessor PID algorithm to independently regulate chamber temperature and relative humidity with high reproducibility. Designed for applications requiring strict adherence to stability protocols—such as biological sample preservation, accelerated stability testing per ICH Q1 guidelines, and material conditioning prior to physical property evaluation—the LHS-250CB maintains a certified working volume of 250 L within a stainless-steel insulated chamber. Its −20 °C to +80 °C operational range and 30–95% RH capability support both low-temperature storage (e.g., plasma, vaccines, cryopreserved cell lines) and elevated-humidity stress testing (e.g., polymer hygroscopicity, pharmaceutical tablet stability). The unit complies with structural and safety requirements outlined in IEC 61010-1 for laboratory equipment and incorporates design features aligned with GLP-compliant environments.

Key Features

• High-efficiency air-cooled refrigeration system using eco-friendly R134a/R406a blended refrigerant, minimizing global warming potential (GWP) and meeting EU F-Gas Regulation Annex I criteria.
• 5.7-inch backlit LCD touchscreen interface with intuitive menu navigation, supporting up to 99 programmable cycles—each comprising up to 30 segments with individual time/temperature/humidity setpoints (max. 99 h 59 min per segment).
• Double-glazed observation door with silicone-sealed inner glass panel ensures thermal insulation integrity while enabling non-intrusive visual monitoring without chamber disturbance.
• Electropolished mirror-finish stainless-steel interior with radius-corner construction facilitates cleaning validation and reduces microbial retention risk—critical for ISO 14644-1 Class 7/8 cleanroom-adjacent use.
• Integrated water reservoir with submerged heating element and auto-refill detection delivers stable humidification; calibrated capacitive humidity sensor (Honeywell or equivalent) provides traceable RH measurement with NIST-traceable calibration options.
• Optimized airflow architecture featuring rear-mounted centrifugal blower and perforated baffle plates ensures ±0.5 °C temperature uniformity (measured at 9 points per IEC 60068-3-5) across the full working volume.
• Adjustable stainless-steel shelving (standard: 3 shelves; max. 19 positions) supports flexible load configuration and meets ASTM E2877-13 requirements for shelf loading distribution in stability chambers.

Sample Compatibility & Compliance

The LHS-250CB accommodates a broad spectrum of sample types—including vials, Petri dishes, tissue culture flasks, electronic components, and polymer specimens—without compromising environmental fidelity. Its internal geometry (580 × 500 × 850 mm) allows placement of standard ISO 9001-certified stability trays and ICH-aligned storage racks. The chamber meets functional equivalency benchmarks referenced in USP , ISO 14644-1 (cleanliness classification), and ASTM E2877-13 (performance verification of environmental chambers). Optional accessories—including 25 mm/50 mm test ports, independent over-temperature alarm module, and BOD-rated GFCI outlets—enable integration into FDA 21 CFR Part 11–compliant workflows when paired with validated USB/RS485 data logging software.

Software & Data Management

Equipped with standard RS485 communication interface (Modbus RTU protocol), the LHS-250CB supports seamless connection to third-party SCADA systems or Shanghai Youyi’s optional PC-based monitoring suite. This software provides real-time graphical trending, automated report generation (PDF/CSV), audit trail functionality with user-level access control, and electronic signature compliance per 21 CFR Part 11 Annex 11. All temperature and humidity logs are timestamped with millisecond precision and stored redundantly—locally on SD card (optional) and remotely via Ethernet-enabled gateway. Calibration history, maintenance records, and deviation alerts are retained in a structured relational database compatible with LIMS integration.

Applications

• Long-term storage of biologics (serum, monoclonal antibodies, viral vectors) under WHO-recommended cold chain conditions (−20 °C ± 3 °C).
• ICH Q1A(R2) and Q5C stability studies for pharmaceuticals, including intermediate condition testing (30 °C/65% RH) and accelerated protocols (40 °C/75% RH).
• Material science preconditioning: moisture equilibration of composites, adhesives, and packaging films prior to tensile, peel, or barrier testing.
• Environmental stress screening of PCB assemblies and semiconductor devices per JEDEC JESD22-A110.
• Microbiological incubation support where sub-ambient temperatures inhibit mesophilic growth while preserving psychrotrophic viability.
• Calibration reference chamber for secondary humidity/temperature sensors used in metrology labs.

FAQ

What is the maximum allowable load mass for optimal thermal uniformity?
For guaranteed ±0.5 °C uniformity across the chamber volume, total load should not exceed 60 kg—including shelves, containers, and samples—to avoid airflow obstruction and thermal mass overload.
Is the unit suitable for continuous operation under GMP conditions?
Yes—when equipped with the optional independent high-limit thermostat and connected to a validated data logger, the LHS-250CB satisfies Annex 15 qualification requirements for long-term operational reliability and alarm response verification.
Can humidity be maintained below 30% RH?
No—the system’s design minimum is 30% RH due to condensation management constraints in the humidification subsystem; desiccant-based dehumidification modules are available as custom-engineered add-ons.
Does the chamber support IQ/OQ/PQ documentation packages?
Shanghai Youyi provides standardized IQ/OQ templates compliant with ISO/IEC 17025 and ASTM E2500-13; PQ execution requires site-specific sensor mapping and protocol development by qualified validation engineers.
What maintenance intervals are recommended for sustained performance?
Compressor oil and refrigerant pressure checks every 24 months; humidity sensor recalibration annually; condenser coil cleaning quarterly in dusty environments; door gasket inspection semiannually.