Elite P230p High-Pressure Preparative Liquid Chromatography System

Overview

The Elite P230p High-Pressure Preparative Liquid Chromatography System is an engineered solution for laboratory-scale purification and isolation of target compounds from complex mixtures. Based on high-pressure gradient or isocratic elution principles, the system employs dual high-precision reciprocating piston pumps to deliver stable, pulse-minimized solvent flow across a wide dynamic range (0.10–40.00 mL/min). It operates under controlled backpressure conditions up to 30 MPa—enabling efficient separation on semi-preparative columns with internal diameters ranging from 10 mm to 50 mm. The system integrates a UV-Vis detector (UV230+, 190–720 nm) equipped with preparative flow cells, a manually or motorized injection valve (Rehodyne 3725i-038), static mixing modules, and optional fraction collection—forming a complete, modular platform for method development, compound enrichment, natural product isolation, and early-stage API purification.

Key Features

• High-stability dual-piston pump architecture delivering flow precision ≤0.2% RSD and accuracy ≤±1.0% under load (10 mL/min, 8.5 MPa, water)
• Wide pressure capability: 30 MPa at low-to-mid flow rates (0.01–20.00 mL/min); 20 MPa at higher flows (20.01–40.00 mL/min), supporting diverse column chemistries and mobile phase viscosities
• UV230+ dual-lamp detector with deuterium and tungsten sources ensures broad spectral coverage (190–720 nm) and consistent photometric response across preparative flow paths
• Modular configuration supports both gradient and isocratic operation; includes dedicated semi-preparative mixer and pressure-rated tubing throughout the fluidic path
• EC2000 chromatographic data system features full Chinese GUI and built-in GLP-compliant audit trail functionality—including user login tracking, method versioning, and raw data integrity safeguards
• Expandable architecture: compatible with external fraction collectors (time- or peak-triggered), column switching valves, and auxiliary detectors (e.g., ELSD, MS interfaces via analog/digital output)

Sample Compatibility & Compliance

The P230p accommodates a broad spectrum of sample matrices including synthetic intermediates, botanical extracts, fermentation broths, and peptide libraries. Its robust stainless-steel fluidic path and chemically resistant seals support use with common organic modifiers (acetonitrile, methanol), aqueous buffers (pH 2–8), and mildly corrosive solvents. Column compatibility spans reversed-phase (C18, C8), silica, amino, cyano, and ion-exchange media in 10–50 mm ID formats. The system meets essential laboratory regulatory expectations: EC2000 software supports ALCOA+ data integrity principles; audit trails, electronic signatures, and method locking align with FDA 21 CFR Part 11 readiness for non-clinical studies. While not certified for GMP manufacturing environments, it fulfills GLP requirements for preclinical research, stability testing, and reference standard preparation per ICH Q5B and USP .

Software & Data Management

The EC2000 Chromatography Data Station provides real-time acquisition, baseline correction, peak integration, retention time alignment, and customizable reporting templates—all accessible through an intuitive Chinese-language interface. Raw data are stored in proprietary but exportable binary format (.ecd), with CSV and PDF report generation enabled. System logs record all hardware events (pump start/stop, pressure alarms, detector lamp status), method changes, and user actions with timestamps and operator IDs. Data backups are supported via network drive mapping or USB storage. Optional software upgrades include multi-channel synchronization, custom calibration curve management, and batch sequence scheduling—facilitating reproducible workflows across multiple analysts and instruments.

Applications

• Purification of natural products (alkaloids, flavonoids, terpenes) from plant extracts using C18 or silica columns
• Isolation of synthetic by-products and reaction intermediates for structural confirmation (NMR, MS)
• Preparative-scale chiral separations when coupled with chiral stationary phases and compatible mobile phases
• Generation of high-purity reference standards for analytical method validation (USP , ICH Q2(R2))
• Desalting and buffer exchange of peptides and oligonucleotides prior to lyophilization or bioassay
• Method scouting and scalability assessment—transitioning from analytical to semi-preparative conditions while preserving selectivity

FAQ

What column dimensions are supported by the P230p system?

The system is optimized for semi-preparative columns with internal diameters of 10 mm, 20 mm, 30 mm, 40 mm, and 50 mm. Custom-length columns up to 250 mm are accommodated with appropriate pressure and flow calibration.
Can the P230p be operated in gradient mode?

Yes—the standard configuration includes a gradient mixing module and dual-pump control, enabling linear, step, or multi-segment gradients with programmable slope and dwell volume compensation.
Is the EC2000 software compliant with 21 CFR Part 11?

EC2000 implements core Part 11 elements: electronic signatures, audit trail with immutable records, role-based access control, and data backup protocols. Full compliance requires site-specific validation documentation and administrative SOPs.
What maintenance intervals are recommended for the high-pressure pump?

Piston seal replacement is advised every 6–12 months depending on usage intensity and solvent aggressiveness; check valve cleaning every 200 hours of operation; UV lamp replacement every 1,000–1,500 hours.
Does the system support automated fraction collection?

Yes—external fraction collectors can be triggered via TTL or analog signal outputs from the UV230+ detector, supporting both time-based and peak-threshold collection modes.