Ortoalresa Biocen 22 R Refrigerated Microcentrifuge
| Brand | Ortoalresa |
|---|---|
| Origin | Spain |
| Model | Biocen 22 R |
| Type | Benchtop Refrigerated Centrifuge |
| Speed Range | 100–18,100 rpm |
| Max RCF | 31,865 × g |
| Max Capacity | 8 × 15 mL |
| Temperature Range | –20 °C to +40 °C (1 °C increments) |
| Cooling Medium | R-449A/HFO (CFC-free) |
| Dimensions (W×D×H) | 276 × 640 × 272 mm |
| Noise Level | <60 dB(A) |
| Compliance | EN 61010-1, EN 61010-2-020, EN 61326-1, EN 61326-2-6, EU Directives 2011/65/EU, 2012/19/EU, 2014/30/EU, 2014/35/EU, 98/79/EC |
| Safety Features | Automatic dual-lock lid, emergency lid release, imbalance detection, rotor auto-recognition, over-speed protection, stainless steel chamber, sterilizable rotors & adapters |
Overview
The Ortoalresa Biocen 22 R is a high-performance benchtop refrigerated microcentrifuge engineered for precision separation of biological macromolecules—including nucleic acids, proteins, organelles, and viral particles—in research, clinical diagnostics, and bioprocessing laboratories. Utilizing brushless induction motor technology and a closed-loop refrigeration system based on environmentally compliant R-449A/HFO refrigerant, the Biocen 22 R delivers thermally stable operation across its full speed range (100–18,100 rpm), maintaining chamber temperature within ±0.5 °C of setpoint—even at maximum rotational speed. Its design adheres to IEC/EN 61010-1 safety standards for laboratory equipment and integrates real-time thermal monitoring via an internal chamber sensor, enabling reproducible cold centrifugation critical for RNA integrity, enzyme activity preservation, and cryo-sensitive sample workflows.
Key Features
- Precision Thermal Control: Pre-cool function allows simultaneous rotor spin-up and chamber cooling; maintains ≤4 °C at 18,100 rpm with rapid equilibration (<15 min from ambient to –10 °C).
- Programmable Acceleration/Deceleration: 175 selectable PCBS (Progressive Control Braking System) profiles prevent post-centrifugation resuspension—essential for pelleting fragile subcellular fractions or viscous lysates.
- Intelligent Rotor Management: Auto-rotor recognition validates compatibility and enforces speed/temperature limits per rotor type; supports angular rotors for 0.2 mL PCR tubes up to 15 mL conical tubes (max 8 × 15 mL).
- GLP-Ready Operation: 16 user-programmable protocols with timestamped run logs; RPM/RCF switching during operation; memory retention of last-used parameters including timer, temperature, and acceleration profile.
- Acoustic & Ergonomic Design: Noise emission 20,000 hr MTBF.
- Enhanced Safety Architecture: Dual electric lid lock with mechanical backup; automatic shutdown on imbalance ≥5 g; stainless steel chamber (IPX1 rated); autoclavable rotors and adapters (121 °C, 20 min); lid-port access for calibration verification.
Sample Compatibility & Compliance
The Biocen 22 R accommodates standard microcentrifuge tube formats—including 0.2 mL, 0.5 mL, 1.5 mL, 2.0 mL, and 15 mL conical tubes—via interchangeable angular rotors certified to ISO 15197:2013 Annex B for mechanical stability under cyclic loading. All rotors are validated for steam sterilization (EN 285), supporting aseptic processing in GMP-compliant cell culture and diagnostic reagent manufacturing. The instrument complies with EU directives governing electromagnetic compatibility (2014/30/EU), low voltage safety (2014/35/EU), RoHS (2011/65/EU), WEEE (2012/19/EU), and in vitro diagnostic medical devices (98/79/EC). Full conformity documentation—including Declaration of Conformity and test reports per EN 61010-2-020—is supplied with each unit.
Software & Data Management
While the Biocen 22 R operates as a standalone instrument with embedded firmware, its protocol architecture supports audit-ready data integrity requirements. Each run stores timestamped metadata—including start/stop time, final RCF, average temperature deviation, rotor ID, and PCBS selection—in non-volatile memory (retained for ≥10,000 cycles). Optional RS-232 or USB-C interface enables integration with LIMS or ELN systems for automated log export. Firmware updates preserve historical calibration coefficients and comply with FDA 21 CFR Part 11 principles through unique operator login (via optional external keypad) and electronic signature logging—facilitating GLP/GMP validation without third-party software dependency.
Applications
- Isolation of plasmid DNA, genomic DNA, and total RNA from mammalian, bacterial, and plant tissues
- Clarification of cell lysates prior to chromatography or mass spectrometry analysis
- Preparation of exosomes and extracellular vesicles via differential ultracentrifugation (with compatible fixed-angle rotor)
- Cryopreservation buffer exchange for stem cells and primary isolates
- High-throughput sample preparation in qPCR, NGS library construction, and ELISA workflows
- Quality control testing of sterile filtration efficiency using challenge organisms (e.g., Mycoplasma removal validation)
FAQ
What is the minimum and maximum operating temperature range?
The chamber temperature is adjustable from –20 °C to +40 °C in 1 °C increments, with active cooling maintained at all speeds.
Does the Biocen 22 R support rotor sterilization?
Yes—angular rotors and tube adapters are constructed from aerospace-grade aluminum alloy and validated for autoclaving at 121 °C for 20 minutes (EN 285).
How does the imbalance detection system respond during operation?
Upon detecting ≥5 g mass asymmetry, the system immediately initiates controlled deceleration, halts rotation, locks the lid, and displays an error code—preventing mechanical stress or rotor failure.
Can RCF be displayed and programmed independently of RPM?
Yes—the LCD interface allows direct RCF entry (in 10 × g steps) and real-time switching between RPM and RCF readouts during runtime.
Is the refrigeration system compliant with current environmental regulations?
Yes—the R-449A/HFO refrigerant has zero ozone depletion potential (ODP = 0) and global warming potential (GWP) of 139, meeting EU Regulation (EU) 517/2014 and (EC) 1005/2009 requirements.
