HAS-100A Desktop Airborne Microbial Sampler

Overview

The HAS-100A Desktop Airborne Microbial Sampler is a compact, portable active air sampler engineered for quantitative collection of viable airborne microorganisms—including bacteria, yeasts, and molds—onto standard agar-filled Petri dishes. It operates on the principle of slit-to-agar impactor sampling: ambient air is drawn at a calibrated volumetric flow rate (100 L/min) through a precision-machined slit nozzle, accelerating particles toward a rotating agar surface. Under controlled impaction velocity, viable microorganisms retain culturability upon deposition, enabling subsequent colony-forming unit (CFU) enumeration in accordance with ISO 14698-1, EU GMP Annex 1, and USP . Designed for routine environmental monitoring in controlled environments, the HAS-100A delivers reproducible, traceable sampling performance without requiring external vacuum sources or complex calibration infrastructure.

Key Features

• Integrated high-efficiency brushless DC motor ensures stable 100 L/min airflow across voltage fluctuations (100–240 V AC, 50–60 Hz), meeting IEC 61000-4-3 immunity requirements.
• Adjustable agar plate rotation (0–4 rpm) enables uniform particle distribution across the entire 90 mm Petri dish surface—critical for statistical validity in low-bioburden environments such as Grade A laminar flow hoods or isolators.
• Backlit dot-matrix LCD display provides real-time feedback on remaining sampling volume, elapsed time, battery status, and user-configurable delay start (0–30 min), supporting unattended operation during shift transitions.
• Rechargeable nickel-metal hydride (Ni-MH) battery pack supports up to 8 hours of continuous sampling on a single charge, validated per IEC 62133 safety standards; low-power firmware automatically disables backlight after 30 s of inactivity and initiates full shutdown after 5 minutes in standby mode.
• Ergonomic rotating sampling head allows precise angular alignment relative to airflow direction—essential for ISO 14644-1 classified cleanrooms where directional sampling protocols must comply with site-specific risk assessments.
• All wetted surfaces contacting sampled air are constructed from medical-grade stainless steel (ASTM F899) and anodized aluminum, minimizing particle shedding and enabling alcohol-based disinfection between uses without material degradation.

Sample Compatibility & Compliance

The HAS-100A is compatible with standard 90 mm Petri dishes containing TSA, SDA, or other microbiologically defined media. Its impactor geometry has been verified to maintain ≥90% collection efficiency for particles ≥0.65 µm aerodynamic diameter (tested per ISO 21501-4 using polystyrene latex spheres). The instrument meets electromagnetic compatibility (EMC) requirements per EN 61326-1 for laboratory use and conforms to RoHS Directive 2011/65/EU. While not certified under FDA 21 CFR Part 11, its operational logs—including date/time stamps, total sampled volume, and rotation speed settings—are exportable via USB for inclusion in GLP/GMP audit trails when integrated into validated environmental monitoring programs.

Software & Data Management

The HAS-100A operates as a standalone hardware platform with no embedded software or network interface. All configuration parameters and runtime data are stored locally in non-volatile memory and displayed directly on the LCD. For regulatory documentation, users record sampling metadata—including location ID, operator initials, sampling duration, and plate lot number—in paper-based or LIMS-integrated electronic batch records. The device supports manual verification of flow accuracy using a primary-standard dry gas meter (e.g., Brooks 5850E) at installation and annually per ISO/IEC 17025 calibration schedules.

Applications

• Environmental monitoring in aseptic manufacturing suites (Grade A/B/C/D) per EU GMP Annex 1 and PIC/S PI 007-6.
• In-process bioburden assessment during sterile filtration validation and media fill simulations.
• Baseline microbial mapping of hospital operating theaters, isolation rooms, and pharmaceutical R&D laboratories.
• Validation of HVAC system performance and filter integrity testing in clean facilities.
• Field-deployable surveillance in food processing plants, veterinary clinics, and agricultural biosecurity zones.

FAQ

Does the HAS-100A require annual recalibration?
Yes—volumetric flow accuracy must be verified annually against a NIST-traceable flow standard, and rotation speed should be confirmed with a handheld tachometer per internal SOPs aligned with ISO/IEC 17025.

Can the HAS-100A be used in explosive atmospheres?
No. It is not rated for ATEX or IECEx hazardous area classification and must only be operated in non-classified, well-ventilated indoor environments.

Is the agar plate rotation speed programmable per sampling event?
Yes. Users select rotation speed (0–4 rpm in 0.5 rpm increments) prior to each run via the keypad interface; settings persist until manually changed.

What maintenance is required between sampling sessions?
After each use, the slit nozzle and impaction surface must be cleaned with 70% isopropyl alcohol and lint-free wipes. No lubrication or disassembly is required under normal operating conditions.

Does the HAS-100A support remote data logging or wireless connectivity?
No. It is a Class II standalone device with no Bluetooth, Wi-Fi, or cloud integration capabilities—designed explicitly for simplicity, reliability, and regulatory transparency in GxP environments.