AB SCIEX TripleTOF 5600+ System (Refurbished)

Overview

The AB SCIEX TripleTOF 5600+ is a refurbished, fully validated high-resolution hybrid quadrupole-time-of-flight (Q-TOF) mass spectrometer engineered for simultaneous qualitative identification and quantitative analysis in liquid chromatography–mass spectrometry (LC-MS) workflows. Unlike conventional triple quadrupole (QqQ) platforms optimized solely for targeted quantitation, the TripleTOF 5600+ leverages orthogonal acceleration time-of-flight (oa-TOF) technology with a dual-stage reflectron and high-transmission Q0 collision cell to deliver untargeted full-scan MS and MS/MS data at high mass accuracy, high resolution, and high sensitivity—without sacrificing speed or dynamic range. Its architecture supports both data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes, enabling deep proteomic profiling, metabolite annotation, impurity characterization, and structural elucidation in compliance with regulatory expectations for analytical method development and verification.

Key Features

• Hybrid Q-TOF platform combining a triple quadrupole front-end (Q1–q2–Q3) with a high-resolution time-of-flight analyzer for true MS/MS capability in full-scan mode
• SmartSpeed™ acquisition engine enabling up to 100 full-scan MS/MS spectra per second—critical for coupling with ultra-fast nanoLC gradients (e.g., Eksigent oLC systems)
• Mass resolution up to 40,000 FWHM at m/z 950 and 25,000 FWHM at m/z 100, verified per IUPAC-defined peak width criteria
• External mass calibration stability: ≤1 ppm RMS mass error over 24 hours using EasyMass™ workflow—meets requirements for confident compound identification per METLIN and HMDB spectral libraries
• Linear dynamic range exceeding four orders of magnitude, validated across small molecules and peptides using isotopically labeled internal standards
• Integrated collision-induced dissociation (CID) with tunable energy control and post-source decay (PSD) support for structural confirmation
• Refurbishment includes replacement of critical consumables (ion guides, detector assemblies, vacuum pumps), full system bake-out, and performance validation against NIST-traceable standards

Sample Compatibility & Compliance

The TripleTOF 5600+ accepts standard electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources, and is compatible with nanoflow, microflow, and conventional HPLC flow rates (50 nL/min–2 mL/min). It supports direct infusion, LC-coupled analysis, and MALDI-TOF/TOF mode via optional source retrofit. All refurbished units undergo full GLP-aligned operational qualification (OQ) including ion transmission efficiency, mass axis linearity, resolution mapping, and detector response uniformity. Data integrity complies with FDA 21 CFR Part 11 requirements when operated with Analyst TF 1.8+ software featuring electronic signatures, audit trails, and secure user access controls. Instrument documentation includes IQ/OQ reports, calibration certificates, and traceable maintenance logs—fully auditable for ISO/IEC 17025, USP , and ICH M10 environments.

Software & Data Management

Controlled by Analyst TF 1.8 or higher, the system supports vendor-native processing pipelines for peptide identification (ProteinPilot™), metabolite profiling (MarkerView™), and quantitative workflows (MasterView™). Raw data (.wiff format) is fully compatible with open-source platforms including ProteoWizard, Skyline, and OpenMS. All refurbished units ship with licensed software bundles, preconfigured method templates, and validated spectral libraries (UniProtKB/Swiss-Prot, LipidBlast, mzCloud). Data storage follows hierarchical retention policies aligned with ALCOA+ principles: raw files archived on encrypted NAS, processed results exported in .csv/.xlsx with embedded metadata, and audit trails retained for ≥2 years.

Applications

• Bottom-up and top-down proteomics—including PTM mapping, cross-linking, and intact protein analysis
• Untargeted metabolomics and lipidomics in biological fluids, tissue extracts, and cell lysates
• Impurity profiling and degradation product identification per ICH Q3B(R2) guidelines
• Biopharmaceutical characterization: glycosylation analysis, deamidation monitoring, sequence variant detection
• Environmental screening for emerging contaminants (e.g., PFAS, pharmaceutical residues) using suspect- and non-target screening workflows
• Forensic toxicology and doping control requiring retrospective data mining and high-confidence library matching

FAQ

Is this instrument eligible for extended warranty or service contracts?

Yes—eligible for SCIEX-certified annual service agreements covering preventive maintenance, remote diagnostics, and priority parts dispatch.
Does the refurbishment include replacement of the TOF detector and MCP assembly?

Yes—detector gain and lifetime are verified during refurbishment; all microchannel plates (MCPs) and anode assemblies are replaced if below 80% of nominal quantum efficiency.
Can the system be upgraded to support SWATH® Acquisition or HDMS^E?

Yes—the TripleTOF 5600+ firmware and Analyst TF software support SWATH® Acquisition v2.0 and HDMS^E workflows out-of-the-box.
What documentation accompanies the instrument?

Full IQ/OQ reports, calibration certificates (NIST-traceable masses), vacuum integrity logs, software license keys, and a signed refurbishment statement from SpectraLab Scientific’s ISO 9001-certified facility.
Is source cleaning and tuning performed prior to shipment?

Yes—each unit undergoes full ESI source disassembly, ultrasonic cleaning, electrode polishing, and automated tuning using reserpine and sodium formate standards prior to final performance verification.