Advion Interchim Scientific puriFlash® 5.020 Preparative Flash Chromatography System

Overview

The Advion Interchim Scientific puriFlash® 5.020 is a fully integrated, benchtop preparative flash chromatography system engineered for reliable, scalable, and method-robust purification of organic compounds, natural products, peptides, and synthetic intermediates in research and early-development laboratories. Built upon Interchim’s decades of expertise in stationary-phase chemistry and fluidic architecture, the system employs low-dispersion gradient pumping, real-time UV/Vis detection (200–400 nm), and intelligent fraction collection to deliver high-resolution separations with excellent reproducibility. Unlike analytical HPLC systems, the puriFlash® 5.020 operates under medium-pressure conditions (up to 20 bar), enabling rapid loading of crude mixtures onto standard silica or specialty cartridges (10 g to 340 g) while maintaining column integrity and minimizing backpressure-induced band broadening. Its modular design supports both isocratic and linear gradient elution modes, making it suitable for routine purification tasks as well as method development workflows aligned with ICH Q5A and Q5B guidelines for impurity profiling.

Key Features

• High-flow dual-piston solvent delivery capable of precise gradients at up to 300 mL/min, ensuring efficient solvent consumption and reduced run times without compromising resolution.
• Integrated UV/Vis detector with variable-wavelength capability (200–400 nm) and digital signal processing for accurate peak tracking and baseline stability across diverse compound classes.
• Automated fraction collector with programmable trigger logic (UV threshold, time, or volume-based), compatible with standard rack formats (13 mm, 16 mm, 18 mm, 21 mm tubes) and custom vial trays.
• Touchscreen control interface running Interchim’s puriSoft™ 5.0 software—designed for intuitive method creation, real-time monitoring, and audit-ready data logging.
• Modular column oven (optional) supporting temperature-controlled separations from ambient to 60 °C, enhancing selectivity for thermally sensitive or highly viscous samples.
• Robust fluidic architecture featuring chemically resistant PEEK and sapphire components, validated for compatibility with common organic solvents including DCM, EtOAc, MeOH, ACN, and THF.

Sample Compatibility & Compliance

The puriFlash® 5.020 accommodates a wide range of sample matrices—including reaction mixtures, crude extracts, and post-coupling peptide solutions—without requiring extensive pre-filtration. Its flow path is optimized for particulate tolerance up to 5 µm, and the system includes an inline 0.45 µm solvent filter and optional 0.22 µm sample filtration module. From a regulatory standpoint, the platform supports GLP-compliant operation through puriSoft™ 5.0’s electronic signature functionality, user-access controls, and full audit trail generation (per FDA 21 CFR Part 11 requirements). Method transfer between puriFlash® systems is facilitated by retention time normalization algorithms embedded in the software, aligning with ASTM E2797-21 standards for chromatographic method equivalence assessment.

Software & Data Management

puriSoft™ 5.0 provides a secure, Windows-based environment for method setup, instrument control, and post-run analysis. All acquisition parameters—including gradient profiles, detector settings, fraction triggers, and pump calibration logs—are stored in encrypted .pfs project files. Raw chromatograms are saved in vendor-neutral ASCII format for third-party integration with LIMS or ELN platforms (e.g., LabArchives, Benchling). The software includes built-in tools for peak integration, purity estimation (area-normalized), and overlay comparison of multiple runs—critical for QC release testing and batch-to-batch consistency evaluation. Data backups are configurable via network drive mapping or scheduled USB export, ensuring continuity during long-duration purification campaigns.

Applications

• Purification of small-molecule APIs and synthetic intermediates in medicinal chemistry support labs.
• Isolation of bioactive constituents from plant and microbial extracts in natural product discovery pipelines.
• Cleanup of Suzuki, Heck, and Buchwald-Hartwig coupling reactions prior to NMR or MS characterization.
• Desalting and buffer exchange of modified oligonucleotides and cyclic peptides using reversed-phase or ion-exchange cartridges.
• Scale-up studies bridging analytical TLC/HPLC methods to milligram- to gram-scale purification.
• Support for DOE-driven solvent screening and gradient optimization in formulation development.

FAQ

What cartridge sizes are supported on the puriFlash® 5.020?
Standard disposable cartridges ranging from 10 g to 340 g (Interchim RediSep® Rf series) and reusable glass columns (15–50 mm ID) are fully compatible.
Can the system be integrated into an automated lab workflow?
Yes—the puriFlash® 5.020 supports RS-232 and Ethernet communication protocols, enabling remote start/stop commands and status polling via external scripting (Python, LabVIEW) or scheduling software.
Is method validation documentation available?
Interchim provides IQ/OQ documentation templates and performance qualification protocols compliant with ISO/IEC 17025 and GMP Annex 15 frameworks.
What maintenance intervals are recommended?
Pump seal replacement every 6 months (or after 1,000 hours of operation), UV lamp replacement annually, and quarterly calibration of flow and pressure sensors using NIST-traceable standards.
Does the system support two-dimensional flash chromatography?
Not natively; however, sequential orthogonal separations can be implemented using fraction re-injection and method chaining within puriSoft™ 5.0.