Agilent 1220 Infinity II Liquid Chromatography System (Refurbished)

Overview

The Agilent 1220 Infinity II Liquid Chromatography System is a fully integrated, refurbished high-performance liquid chromatograph engineered for routine analytical laboratories requiring robustness, regulatory readiness, and long-term operational stability. Based on Agilent’s InfinityLab LC platform architecture, this system employs conventional high-pressure liquid chromatography (HPLC) principles—utilizing positive displacement solvent delivery, precise sample introduction, thermally controlled column elution, and UV-Vis absorbance detection—to deliver reproducible retention time precision, peak area repeatability, and baseline stability across diverse sample matrices. Its 600 bar pressure capability supports sub-2 µm particle-packed columns, enabling higher resolution separations and reduced analysis times without compromising system integrity. As a pre-owned instrument certified and reconditioned to original equipment manufacturer (OEM) specifications, it undergoes full functional validation—including pump flow accuracy, gradient composition verification, detector linearity and noise assessment, temperature uniformity testing, and software integration checks—prior to release.

Key Features

• Integrated modular design with factory-tested configurations: isocratic or quaternary gradient pump options, thermostatted column compartment (ambient to 80 °C), variable wavelength detector (190–600 nm, 80 Hz acquisition), and choice of manual injector or 100-position autosampler.
• Regulatory-compliant architecture supporting method validation requirements per USP , ICH Q2(R2), and ISO/IEC 17025:2017; firmware and software configurable for 21 CFR Part 11 compliance when deployed with OpenLAB CDS and appropriate access controls.
• Walk-up operation enabled via external sample tray and dedicated walk-up software interface—ideal for shared laboratory environments where multiple users require independent method execution without administrative intervention.
• Expandable platform: compatible with InfinityLab series auxiliary modules including refractive index (RID), fluorescence (FLD), and evaporative light scattering (ELSD) detectors, as well as direct coupling to Agilent 6000-series single quadrupole mass spectrometers for hyphenated LC-MS workflows.
• Low total cost of ownership: engineered for minimal daily maintenance—no degasser service intervals, self-diagnostic pump heads, sealed optical path in VWD, and simplified fluidic routing reduce downtime and consumables dependency.

Sample Compatibility & Compliance

The 1220 Infinity II accommodates standard 4.6 × 150 mm HPLC columns and supports reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatography applications. It handles aqueous-organic mobile phases (e.g., water/acetonitrile, water/methanol), buffers (e.g., phosphate, acetate), and low-concentration acid/base modifiers. All hardware components comply with CE marking directives and RoHS 2011/65/EU. The system meets essential performance criteria outlined in ASTM D5502 (for hydrocarbon analysis), ISO 11843-2 (detection limit estimation), and EP 2.2.46 (chromatographic system suitability). Refurbishment includes replacement of critical wear items—pump seals, injection valve rotor seals, detector flow cells—and recalibration traceable to NIST-traceable standards.

Software & Data Management

Controlled exclusively via Agilent OpenLAB Chromatography Data System (CDS), the system supports two validated software editions: EZChrom (optimized for simplicity and SOP-driven workflows) and ChemStation (designed for advanced method development, multi-instrument control, and custom reporting). Both versions provide electronic signatures, user role-based permissions, full audit trail logging, raw data integrity protection (SHA-256 hashing), and secure database archiving. Instrument methods, sequences, and results are stored in a centralized Oracle or Microsoft SQL Server repository, ensuring ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) data governance alignment.

Applications

This system is routinely deployed in pharmaceutical QC labs for assay, dissolution, and impurity profiling per USP monographs; in food safety testing for pesticide residue screening (AOAC 2007.01); in environmental labs for PAH and PCB quantification per EPA Method 8330B; and in academic research for natural product fractionation and polymer molecular weight distribution analysis. Its pressure and detection sensitivity make it suitable for method transfer from legacy Agilent 1100/1200 systems, minimizing revalidation burden.

FAQ

Is this unit covered by Agilent’s original warranty?
No—this is a refurbished system backed by a 12-month limited warranty provided by the authorized distributor, covering parts and labor for defects in materials and workmanship.
Are calibration certificates included?
Yes—each unit ships with a Certificate of Reconditioning and Performance Verification, including documented test results for flow accuracy (±0.5%), gradient error (<0.5% RSD), column oven temperature stability (±0.2 °C), and detector noise (<±2.5 × 10⁻⁵ AU).

Can this system be connected to a networked LIMS?
Yes—OpenLAB CDS supports standard ASTM E1384 and HL7 interfaces for bidirectional data exchange with laboratory information management systems.
What documentation is supplied?
Full set of English-language user manuals, safety instructions, installation qualification (IQ) checklist, and preventive maintenance schedule—all delivered digitally and on USB drive.
Is remote support available after purchase?
Yes—technical assistance is provided via email, screen-sharing sessions, and phone during business hours (EST), with optional extended support contracts available upon request.