Agilent 1260 Infinity II Used Liquid Chromatograph

Overview

The Agilent 1260 Infinity II Liquid Chromatograph is a high-performance, modular HPLC system engineered for precision, robustness, and long-term operational stability in regulated and research-intensive laboratory environments. Built upon Agilent’s proven Infinity Architecture, this reconditioned system employs low-dispersion fluidic pathways, active solvent pre-mixing, and pressure-optimized flow control to deliver exceptional retention time reproducibility (<0.05% RSD), baseline stability, and gradient accuracy across extended run sequences. As a certified pre-owned instrument, each unit undergoes full functional validation—including pump linearity and pulse-free flow verification, UV/VIS detector wavelength accuracy and noise assessment (≤ ±1 nm, < ±0.5 × 10⁻⁵ AU), autosampler carryover testing (< 0.005%), and column oven temperature uniformity mapping—per Agilent’s Service Quality Assurance Protocol. The system is fully compatible with U.S. Pharmacopeia (USP) <621>, ISO/IEC 17025, and FDA 21 CFR Part 11-compliant data integrity workflows when deployed with validated OpenLAB CDS software.

Key Features

• Quaternary high-pressure gradient pump delivering flow rates from 0.001–10.0 mL/min with ≤ 0.1% RSD flow precision and pressure capability up to 600 bar
• Variable Wavelength Detector (VWD) or Diode Array Detector (DAD) with spectral range 190–950 nm, 1 nm resolution, and real-time peak purity analysis
• Thermostatted autosampler with 100-position capacity, precise 4–40 °C temperature control, and programmable injection volume (0.1–100 µL)
• Column compartment supporting dual-column configuration, temperature range 5–80 °C (±0.15 °C stability), and integrated pressure monitoring
• Fully refurbished fluidic path: all seals, check valves, purge valves, and capillary tubing replaced; pump heads polished and flow cell cleaned per Agilent service bulletin 5991-3225EN
• Factory-reset firmware and validated OpenLAB CDS 2.x software installation with audit trail, electronic signature, and user access level management

Sample Compatibility & Compliance

The Agilent 1260 Infinity II supports a broad range of sample matrices including pharmaceutical actives and excipients, environmental water extracts, food additives, natural product isolates, and polymer degradation products. It is routinely employed in methods compliant with ASTM D8077 (HPLC analysis of biodiesel impurities), USP <621> Chromatography, EP 2.2.46, and ICH Q2(R2) validation guidelines. All refurbished units are delivered with a Certificate of Conformance detailing calibration history, performance qualification (PQ) test results, and traceability to NIST-traceable standards. System suitability testing (SST) parameters—including theoretical plates (N), tailing factor (T), resolution (Rs), and %RSD of replicate injections—are documented and verified prior to shipment.

Software & Data Management

The system operates exclusively with Agilent OpenLAB CDS (Chemical Data System), supporting both legacy ChemStation and modern EZChrom interfaces. Data acquisition, processing, reporting, and archiving are fully governed under ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available). Audit trails record all method changes, sequence modifications, and result reprocessing events with user ID, timestamp, and reason-for-change fields. Electronic signatures comply with 21 CFR Part 11 requirements when configured with Windows Active Directory authentication and role-based permissions. Raw data files (.D format) are stored in secure, encrypted repositories with automated backup scheduling and version-controlled method libraries.

Applications

This instrument serves critical roles in quality control laboratories performing assay quantitation (e.g., ibuprofen content per USP-NF), impurity profiling (ICH Q3B), residual solvent analysis (ICH Q3C), and stability-indicating method development. In environmental labs, it enables EPA Method 8330B (PAHs in soil/sediment) and ASTM D7089 (petroleum hydrocarbon fractions). Food safety applications include pesticide residue screening (EU SANTE/11312/2021), mycotoxin detection (aflatoxin B1/B2/G1/G2), and vitamin analysis (AOAC 2002.01). Academic and contract research organizations utilize its modularity for method transfer studies, column screening, and preparative-scale fraction collection via optional fraction collector integration.

FAQ

Is this instrument eligible for Agilent’s official warranty and service support?
Yes—each unit carries a 12-month comprehensive parts-and-labor warranty backed by Agilent-certified field service engineers and includes priority access to Agilent Technical Support.
Can the system be upgraded to support UHPLC capabilities?
No—the 1260 Infinity II is an HPLC-class platform with maximum pressure rating of 600 bar; UHPLC operation (≥1000 bar) requires the 1290 Infinity II architecture.
What documentation accompanies the instrument upon delivery?
You receive a complete package: Factory Refurbishment Report, Performance Qualification Summary, Certificate of Conformance, OpenLAB CDS Installation & Validation Summary, and Agilent’s Standard Operating Procedure (SOP) templates for PQ and routine maintenance.
Does the system include a computer and monitor?
Yes—a dedicated, validated Windows 10 Pro workstation with pre-installed OpenLAB CDS, licensed software, and network configuration is included.
Are spare parts and consumables available post-purchase?
All genuine Agilent consumables—including G1314F VWD flow cells, G1312B quaternary pump seals, and G1329B autosampler rotor seals—are stocked and supplied with first-year maintenance kits.