Agilent 1290 Infinity HTC/HTS Autosampler (Refurbished)

Overview

The Agilent 1290 Infinity HTC/HTS Autosampler is a high-performance, refurbished liquid chromatography autosampler engineered for ultra-high-throughput and ultra-low carryover applications—particularly in LC-MS, quantitative bioanalysis, clinical research, and regulated pharmaceutical environments. It operates on the Dynamic Load and Wash (DLW) principle, a proprietary fluidic architecture that separates sample aspiration, needle washing, and injection into sequential, non-overlapping stages—minimizing cross-contamination and achieving residual levels consistently below 0.001%. Designed as a core component of the Agilent 1290 Infinity II LC platform, this autosampler supports pressure-rated operation up to 1200 bar, enabling compatibility with sub-2-µm particle columns and ultrafast gradient separations. Its robust mechanical design, precision stepper-motor-driven XYZ motion system, and sealed, inert fluid path (including sapphire needle seat and PEEK/MP35N wetted materials) ensure long-term reproducibility and chemical resistance across diverse solvent and biological matrix conditions.

Key Features

• Dynamic Load and Wash (DLW) architecture—eliminates passive wash inefficiencies by actively purging the needle interior and exterior in real time using programmable solvent gradients and high-velocity rinse cycles
• 1200-bar-rated valve manifold—engineered for UHPLC-grade pressure stability and minimal dwell volume (< 15 µL), supporting method transfer from HPLC to UHPLC without re-optimization
• Refrigerated sample compartment (4–10 °C)—maintains thermal integrity for thermally labile compounds, including peptides, oligonucleotides, and metabolites; temperature uniformity ±0.5 °C across all positions
• High-density sample handling—supports up to 24 standard 96-well or 384-well microplates simultaneously, with configurable plate recognition and barcode scanning (optional)
• Fast cycle time—typical injection-to-injection time of ≤18 seconds under standard LC-MS conditions, scalable via parallel wash and pre-load sequencing
• Modular service design—field-replaceable syringe, needle, seal pack, and valve cartridge kits reduce downtime and support GLP-compliant maintenance logging

Sample Compatibility & Compliance

The HTC/HTS autosampler accommodates a broad range of sample formats—including vials (2–4 mL), screw-top and crimp-top, 96-/384-well plates, and custom tube racks—with automatic depth sensing and adaptive needle penetration. All wetted surfaces comply with USP Class VI and ISO 10993 biocompatibility standards. The system meets key regulatory requirements for data integrity and audit readiness: full support for 21 CFR Part 11-compliant electronic signatures, audit trails, and user access control when operated through Agilent OpenLab CDS or MassHunter Quantitative Analysis software. It is routinely validated per ICH Q2(R2) guidelines for precision (RSD < 0.5% for retention time, < 1.0% for peak area), linearity (r² ≥ 0.999 over 3-log dynamic range), and carryover (verified per USP and ASTM D7592 protocols).

Software & Data Management

Fully integrated with Agilent’s enterprise chromatography data systems—including OpenLab CDS 2.x, MassHunter Workstation (B.08+), ChemStation Rev. B.04.03+, and EZChrom Elite—the autosampler enables method-driven sequence setup, real-time status monitoring, and automated calibration reporting. Instrument methods store complete DLW parameters (wash solvent composition, flow rate, dwell time, rinse volume), ensuring full traceability and method reproducibility across laboratories. Audit trail records include timestamped entries for every injection event, parameter change, error condition, and user action—exportable in CSV or PDF for internal QA review or regulatory submission. Remote diagnostics and firmware update capability are supported via Agilent’s SecureConnect protocol.

Applications

This refurbished 1290 HTC/HTS autosampler is routinely deployed in high-volume analytical workflows requiring stringent carryover control and method robustness: pharmacokinetic (PK) and toxicokinetic (TK) sample analysis in contract research organizations (CROs); high-throughput screening (HTS) of compound libraries in drug discovery; residue testing in food safety and environmental labs (e.g., pesticide multiresidue analysis per EN 15662); and biomarker quantification in clinical proteomics and metabolomics studies. Its low-temperature sample handling and DLW performance make it especially suitable for unstable analytes such as phosphorylated proteins, labile glucuronide conjugates, and short-chain fatty acids.

FAQ

Is this unit fully refurbished and tested prior to shipment?
Yes—each unit undergoes full functional validation including pressure leak testing, carryover verification (using caffeine/acetonitrile challenge), temperature calibration, and injection precision assessment per Agilent’s Factory Acceptance Test (FAT) protocol.
Does the 6-month warranty cover parts and labor?
Yes—the warranty includes onsite or depot-based repair, replacement of defective components, and remote technical support for software configuration and method migration.
Can this autosampler be integrated with non-Agilent LC systems?
It can operate in standalone mode or via TTL/RS-232 triggers, but full bidirectional communication (e.g., status feedback, error reporting) is only guaranteed with Agilent 1200/1260/1290 LC modules and compatible CDS platforms.
Are original Agilent service manuals and calibration certificates included?
Yes—each unit ships with a digital package containing the latest Agilent Service Manual (G1367E), FAT report, and a NIST-traceable temperature calibration certificate.
What documentation is provided for regulatory compliance?
A complete vendor-supplied IQ/OQ documentation package is available upon request, aligned with ASTM E2500-13 and EU Annex 11 principles, including risk assessment, test scripts, and executed reports.