Analytik Jena P25/P25T Electrophoresis Power Supply

Overview

The Analytik Jena P25 and P25T electrophoresis power supplies are precision-engineered DC power sources designed for reliable, reproducible operation in molecular biology and protein biochemistry laboratories. These units operate on the fundamental principle of delivering stable, programmable direct current (DC) voltage and current to drive charged biomolecules—such as DNA, RNA, and proteins—through porous matrices (e.g., agarose or polyacrylamide gels) under controlled electric fields. Engineered for versatility across gel electrophoresis and electroblotting workflows, the P25 series supports both constant-voltage and constant-current modes with seamless auto-switching logic that dynamically maintains user-defined output conditions during runtime—critical for preventing thermal drift, band distortion, or gel overheating during extended runs.

Key Features

• Output range up to 400 V and 1000 mA, enabling compatibility with standard horizontal and vertical slab gels, mini-gels, and large-format systems.
• Dual operating modes: constant voltage (CV) and constant current (CC), with intelligent automatic mode switching to preserve experimental integrity when load resistance changes (e.g., during buffer depletion or gel polymerization).
• Non-volatile memory retains the last-used parameters—including voltage, current, time, and mode—even after power cycling, reducing setup time and minimizing configuration errors between experiments.
• Programmable timer with audible and visual end-of-run indication; prevents overrunning, sample diffusion beyond the gel matrix, and irreversible loss of resolution.
• Four independent output terminals allow simultaneous control of up to four electrophoresis or electrotransfer systems—ideal for high-throughput labs conducting parallel assays or comparative analyses.
• Rugged industrial-grade enclosure with ventilation-optimized thermal management ensures long-term stability under continuous operation at elevated loads.

Sample Compatibility & Compliance

The P25/P25T is validated for use with a broad spectrum of electrophoretic configurations, including but not limited to: horizontal submarine agarose electrophoresis (e.g., for DNA fragment analysis), vertical SDS-PAGE and native PAGE, semi-dry and wet-tank western blot transfer systems, and pulse-field electrophoresis accessories (when used with compatible pulse modules). All units comply with IEC 61010-1:2010 safety standards for laboratory electrical equipment and meet CE marking requirements for electromagnetic compatibility (EMC) per EN 61326-1. The design supports GLP-compliant documentation practices through consistent parameter recall and repeatable output delivery—facilitating audit readiness in regulated environments such as clinical diagnostics or pharmaceutical QC labs.

Software & Data Management

While the P25 and P25T operate via intuitive front-panel controls without mandatory PC connectivity, their parameter retention architecture aligns with data integrity principles outlined in FDA 21 CFR Part 11 and ISO/IEC 17025. Each unit logs no persistent runtime data internally; however, the deterministic behavior—combined with saved settings and timer-based repeatability—enables full traceability when integrated into lab-wide electronic lab notebook (ELN) or LIMS workflows. No proprietary drivers or software installation is required, ensuring compatibility with air-gapped networks common in biosafety level (BSL)-2+ facilities.

Applications

• Genomic DNA and PCR product separation on agarose gels (0.5–2% w/v) at 50–150 V.
• High-resolution protein separation via SDS-PAGE (8–20% acrylamide) using constant-current protocols (10–30 mA per gel).
• Efficient semi-dry and wet electroblotting of proteins onto PVDF or nitrocellulose membranes at optimized field strengths (e.g., 15–25 V for semi-dry, 100 V for wet transfer).
• Electrophoretic mobility shift assays (EMSA) requiring precise low-voltage control (< 100 V) and minimal current fluctuation.
• Training and teaching laboratories where robustness, ease of use, and multi-system support reduce instrument footprint and operational overhead.

FAQ

Does the P25/P25T support constant-power (CP) mode?
No—the unit operates exclusively in constant-voltage and constant-current modes, with automatic transition logic between them based on real-time load impedance.
Can the timer be disabled for continuous operation?
Yes; setting the timer to “0” disables timing functionality, allowing indefinite run duration until manually stopped.
Is remote control or RS-232/USB interface available?
No—these models are standalone benchtop units without digital communication ports. For automated integration, third-party relay-based triggering may be implemented externally.
What safety protections are built into the power supply?
Over-voltage, over-current, over-temperature, and short-circuit protection are embedded in the power regulation circuitry, automatically halting output and triggering visual alarm indicators upon fault detection.
Are replacement fuses and output cables supplied with the unit?
Standard IEC 60127-2 certified fuses (250 V, 1.6 A slow-blow) and insulated banana-to-alligator test leads are included in the shipment package.