AOE V120Pro Single-Beam UV-Vis Spectrophotometer
| Brand | AOE |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | V120Pro |
| Price Range | USD 700–1,400 (FOB) |
| Optical System | Single-Beam with Holographic Grating (1200 lines/mm) |
| Detector | Silicon Photocell |
| Wavelength Range | 320–1100 nm |
| Wavelength Accuracy | ±1.0 nm |
| Wavelength Repeatability | ≤0.2 nm |
| Spectral Bandwidth | 4.0 nm |
| Stray Light | ≤0.1%T at 220 nm and 360 nm |
| Baseline Stability | ±0.001 A/h at 500 nm |
| Wavelength Scanning Mode | Motorized Auto-Scanning |
| Display | 7-inch TFT LCD Touchscreen |
| Data Output | USB 2.0, RS-232, Optional PC Software |
Overview
The AOE V120Pro is a single-beam ultraviolet-visible (UV-Vis) spectrophotometer engineered for routine quantitative and qualitative analysis in academic teaching laboratories, quality control environments, and small-scale R&D settings. It operates on the principle of Beer-Lambert law-based absorbance measurement, utilizing a high-efficiency holographic grating (1200 lines/mm) to disperse polychromatic light into its spectral components. The instrument delivers reliable photometric performance across a broad wavelength range of 320–1100 nm—covering near-UV, visible, and near-infrared regions—enabling applications such as concentration determination of nucleic acids and proteins, colorimetric assays (e.g., Bradford, Lowry), kinetic studies, and standard curve generation. Its single-beam architecture ensures mechanical simplicity and thermal stability, while motorized auto-wavelength scanning eliminates manual adjustment and improves operational consistency.
Key Features
- High-resolution holographic grating (1200 lines/mm) optimized for signal-to-noise ratio and wavelength fidelity
- Automated wavelength scanning with real-time display on a 7-inch capacitive touchscreen interface
- Silicon photocell detector offering robust linearity and low dark-current drift over extended operation
- Narrow 4.0 nm spectral bandwidth ensuring adequate resolution for most conventional colorimetric and biochemical assays
- Wavelength accuracy of ±1.0 nm and repeatability ≤0.2 nm—meeting ISO 8579-1:2020 and ASTM E275-22 verification criteria for routine spectrophotometers
- Baseline stability of ±0.001 A/h at 500 nm, supporting long-duration kinetic measurements without recalibration
- Stray light level ≤0.1%T at critical validation wavelengths (220 nm and 360 nm), minimizing spectral interference in low-transmittance samples
- Integrated USB 2.0 and RS-232 ports for direct data export and remote instrument control
Sample Compatibility & Compliance
The V120Pro accommodates standard 10 mm pathlength quartz or glass cuvettes (including matched pairs for reference/blank compensation). It supports liquid-phase analysis of aqueous, organic, and buffered solutions; suspensions with minimal turbidity; and thin-film-coated substrates when used in reflectance mode (with optional accessory kit). The optical design complies with general requirements outlined in ISO/IEC 17025:2017 for calibration traceability, and its photometric performance aligns with the minimum specifications defined in USP for UV-Vis spectrophotometers used in pharmaceutical testing. While not certified for GMP-regulated production environments, it satisfies GLP-compliant documentation needs when paired with validated software and audit-trail-enabled data acquisition protocols.
Software & Data Management
The V120Pro includes optional PC-based acquisition and analysis software compatible with Windows 10/11 (64-bit). The software supports full-spectrum scanning, time-driven kinetics, multi-wavelength quantitation, and standard curve fitting (linear, quadratic, cubic, and 4-parameter logistic models). All raw absorbance, transmittance, and concentration data are timestamped and stored in CSV and XML formats, enabling integration with LIMS platforms. Audit trail functionality—including user login logs, method version history, and parameter change records—is available upon software license activation, facilitating compliance with FDA 21 CFR Part 11 requirements when deployed in regulated QC labs under documented SOPs.
Applications
- Determination of DNA/RNA concentration and purity (A260/A280 and A260/A230 ratios)
- Enzyme activity assays (e.g., NADH/NADPH oxidation at 340 nm)
- Water quality analysis (nitrate, phosphate, iron, and COD via colorimetric reagents)
- Pharmaceutical excipient and active ingredient quantification per pharmacopoeial methods
- Industrial dye and pigment concentration monitoring in textile and paint manufacturing
- Teaching fundamental spectroscopic concepts including absorption maxima, molar absorptivity, and spectral deconvolution
FAQ
Is the V120Pro suitable for regulatory-compliant pharmaceutical testing?
It meets technical prerequisites for many USP and EP monograph procedures but requires full software validation, instrument qualification (IQ/OQ/PQ), and documented SOPs to support GMP use.
Can it measure absorbance below 320 nm?
No—the optical path and detector sensitivity are optimized for ≥320 nm; sub-UV measurements require a double-beam instrument with deuterium lamp and quartz optics.
What cuvette materials are supported?
Standard 10 mm square cuvettes made of optical glass (320–1100 nm) or fused silica/quartz (down to 190 nm, though not utilized here due to wavelength cutoff).
Does it support kinetic measurements?
Yes—time-scan mode enables up to 10,000 data points per run with adjustable interval timing from 0.1 s to 600 s.
Is wavelength calibration traceable to NIST standards?
Factory calibration uses certified holmium oxide and didymium filters; end users may perform periodic verification using NIST-traceable reference standards (e.g., SRM 2035).
