Beijing Liuyi DYCZ-21 Multi-Application Electrophoresis Apparatus (Model 121-2100)
| Brand | Beijing Liuyi (LY) |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | 121-2100 |
| Instrument Type | Nucleic Acid Electrophoresis System |
| Configuration | Complete Electrophoresis Unit with Integrated Chamber |
| Gel Format | Vertical Slab Gel |
| Maximum Gel Dimensions | 170 × 170 mm |
| External Dimensions (L × W × H) | 240 × 150 × 240 mm |
| Buffer Capacity | 600 mL |
| Weight | 6 kg |
Overview
The Beijing Liuyi DYCZ-21 Multi-Application Electrophoresis Apparatus (Model 121-2100) is a benchtop vertical electrophoresis system engineered for versatility and pedagogical reliability in nucleic acid and protein separation workflows. Based on standard polyacrylamide and agarose gel electrophoresis principles—where charged biomolecules migrate under an applied DC electric field through a porous matrix—the DYCZ-21 supports three primary operational modes: tube gel electrophoresis (e.g., for SDS-PAGE in capillary formats), conventional vertical slab gel electrophoresis, and first-dimension isoelectric focusing (IEF) compatible with subsequent second-dimension SDS-PAGE for two-dimensional electrophoresis (2-DE). Its modular design enables rapid reconfiguration between configurations without tooling, making it particularly suited for teaching laboratories where students require hands-on exposure to foundational electrophoretic techniques under controlled, repeatable conditions.
Key Features
- Triple-mode compatibility: Supports tube gel, vertical slab gel, and preparatory IEF setups for 2-DE workflows.
- Standardized 170 × 170 mm gel casting area—optimized for high-resolution nucleic acid separation and small-scale protein analysis.
- Dual-thickness comb system: Includes 1.0 mm and 1.5 mm spacers with both 20- and 26-well configurations to accommodate varied sample volumes and resolution requirements.
- Integrated buffer chamber with 600 mL total capacity and dual ice-cooling slots (included as standard) to maintain thermal stability during extended runs (e.g., overnight IEF or high-voltage SDS-PAGE).
- Robust acrylic housing with corrosion-resistant electrode assembly and precision-machined glass plate alignment fixtures ensuring consistent gel thickness and uniform electric field distribution.
- Modular accessory set—including sealing gaskets, rubber stoppers (perforated and solid), agarose sealing trays, gel casting stands, and calibrated syringes—enables full experimental autonomy without third-party consumables.
Sample Compatibility & Compliance
The DYCZ-21 accommodates common nucleic acid applications including DNA fragment analysis (100 bp–20 kb), plasmid topology assessment, RNA integrity checks, and PCR product verification. For protein work, it supports native PAGE, SDS-PAGE, and preparatory IEF gels (pH 3–10 immobilized pH gradients). All components comply with general laboratory safety standards for low-voltage (<600 V) DC electrophoresis systems. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11, the apparatus meets baseline functional requirements for GLP-aligned academic and QC environments when operated within specified voltage (up to 600 V) and current (up to 100 mA) limits. Buffer containment design minimizes electrolyte leakage and cross-contamination risks during multi-gel runs.
Software & Data Management
This is a hardware-only electrophoresis platform requiring external power supply (not included). No proprietary software or digital control interface is integrated; operation is fully manual via external DC power source adjustment. Data acquisition relies on post-run imaging using standard UV transilluminators or white-light scanners. The system’s mechanical consistency—verified across repeated gel pours and runs—ensures high inter-assay reproducibility essential for comparative analysis in teaching labs and preliminary research screening. Documentation includes bilingual (Chinese/English) user manual covering gel casting protocols, troubleshooting matrices, and maintenance guidelines aligned with ASTM D4929-18 (standard practice for electrophoresis equipment qualification).
Applications
- Undergraduate and graduate molecular biology teaching labs: DNA ladder calibration, restriction digest verification, and basic protein electrophoresis.
- Quality control of extracted nucleic acids prior to sequencing or cloning workflows.
- Preparative isoelectric focusing for downstream 2-DE in proteomics pilot studies.
- Validation of CRISPR editing efficiency via T7E1 or Surveyor nuclease assays.
- Optimization of agarose concentration and voltage gradient for novel amplicon detection.
FAQ
Is a power supply included with the DYCZ-21 system?
No. A compatible DC power supply capable of delivering up to 600 V / 100 mA must be sourced separately.
Can this unit be used for SDS-PAGE with Coomassie staining?
Yes—its 1.0 mm and 1.5 mm gel thickness options support both high-resolution and high-loading SDS-PAGE applications compatible with standard Coomassie R-250 or G-250 protocols.
Are replacement combs and glass plates available as standalone items?
Yes. All listed accessories—including combs (141-2101 through 141-2104), glass plates (142-2101, 142-2105, 142-2106), and sealing components—are orderable individually via Beijing Liuyi’s global distributor network.
Does the system support temperature-controlled electrophoresis?
It does not feature active thermoregulation; however, the dual ice-cooling slots (412-1002) enable passive temperature stabilization during runs up to 4 hours at ≤200 V.
What safety certifications does the DYCZ-21 hold?
The apparatus conforms to general electrical safety principles for Class I laboratory equipment per IEC 61010-1:2010, though formal certification documentation is provided only upon request for institutional procurement compliance review.
