Beijing Liuyi DYCZ-24B Wide-Format Dual Vertical Electrophoresis Apparatus
| Brand | Beijing Liuyi / LY |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | DYCZ-24B |
| Instrument Type | Protein Electrophoresis Apparatus |
| Gel Plate Dimensions | 130 × 180 mm |
| Overall Dimensions (L × W × H) | 265 × 160 × 185 mm |
| Buffer Capacity | 750 mL |
| Weight | 5 kg |
Overview
The Beijing Liuyi DYCZ-24B Wide-Format Dual Vertical Electrophoresis Apparatus is an engineered solution for high-throughput protein separation in academic research laboratories, quality control environments, and biotechnology R&D settings. Designed around the fundamental principle of vertical polyacrylamide gel electrophoresis (PAGE), this apparatus enables simultaneous electrophoretic migration of two independent SDS-PAGE or native PAGE gels under identical thermal and electrical field conditions. Its dual-gel configuration supports comparative analysis—such as time-course experiments, dose-response studies, or replicate validation—without inter-run variability introduced by sequential runs. The unit operates with standard Tris-Glycine or Bis-Tris buffer systems and is compatible with constant voltage, constant current, or constant power modes when paired with a regulated DC power supply (not included). Thermal management is passively optimized via its compact acrylic chamber geometry and low-resistance electrode assembly, minimizing edge heating and band distortion across the full 130 × 180 mm gel surface.
Key Features
- Dual vertical gel chamber architecture enabling concurrent electrophoresis of two independent 130 × 180 mm gels
- Wide-format gel plate design accommodating up to 52-sample lanes per gel using standardized comb configurations
- Modular glass plate system: includes four concave and four flat tempered glass plates (1.0 mm and 1.5 mm thickness options) for precise gel casting and uniform polymerization
- Low-volume buffer reservoir design (750 mL total capacity) with upper/lower tank configuration, reducing reagent consumption by ~30% compared to conventional single-gel units
- Tool-free assembly/disassembly workflow with silicone sealing strips, stainless-steel clamps (8×), and precision-machined alignment brackets
- Chemically resistant acrylic housing rated for repeated exposure to acrylamide monomers, SDS, methanol, and ethanol-based destaining solutions
Sample Compatibility & Compliance
The DYCZ-24B supports standard molecular weight protein markers (10–250 kDa), phosphorylated isoforms, glycoproteins, and disulfide-bonded complexes when used with appropriate gel concentrations (8–20% T) and running buffers. It accommodates comb formats of 25, 40, and 52 teeth at 1.0 mm or 1.5 mm thickness—enabling optimization for resolution (narrower wells) or loading capacity (wider wells). All components comply with ISO 9001-certified manufacturing protocols. While the apparatus itself does not carry CE or FDA 510(k) designation, its mechanical design and material selection conform to general laboratory equipment safety standards (IEC 61010-1) for Class II, Pollution Degree 2 environments. When integrated into validated workflows, it supports GLP-compliant documentation practices through traceable component part numbers (e.g., 141-3001, 142-2422) and batch-recordable gel casting procedures.
Software & Data Management
As a standalone electrophoresis platform, the DYCZ-24B requires no embedded firmware or proprietary software. All operational parameters—including voltage, current, run duration, and temperature monitoring—are managed externally via a compatible programmable power supply (e.g., Bio-Rad PowerPac HC or Thermo Fisher Ettan EPS 3501). This architecture ensures full compatibility with third-party imaging systems (e.g., Azure Biosystems c600, UVP BioSpectrum) and analysis software such as ImageLab, AlphaView SA, or Fiji/ImageJ. Raw gel images retain native TIFF or PNG export fidelity, supporting audit-ready archiving in accordance with 21 CFR Part 11–aligned data integrity frameworks when paired with compliant LIMS or ELN platforms.
Applications
- Comparative SDS-PAGE analysis of protein expression profiles across multiple cell lysates or tissue extracts
- Validation of western blot transfer efficiency using parallel Coomassie-stained reference gels
- Optimization of gradient gel electrophoresis conditions for complex proteomic samples
- Training and teaching labs requiring robust, repeatable vertical electrophoresis platforms with minimal maintenance overhead
- Preparative-scale protein fractionation prior to downstream mass spectrometry or activity assays
FAQ
Is the DYCZ-24B compatible with pre-cast gels?
No—it is designed exclusively for hand-cast polyacrylamide gels using the supplied glass plates, combs, and sealing accessories.
What power supply specifications are recommended?
A DC power supply capable of 10–500 V, 1–500 mA, and 50 W output is required; constant voltage mode is most commonly used for routine protein electrophoresis.
Can the unit be used for DNA electrophoresis?
While technically feasible, the 130 × 180 mm format exceeds typical nucleic acid gel dimensions; resolution and band sharpness may be compromised without protocol adaptation.
Are replacement parts available internationally?
Yes—core consumables including combs (141-series), glass plates (142-series), and sealing components (143-/144-series) are distributed globally through authorized Beijing Liuyi partners with documented lot traceability.
Does the system include cooling capability?
No active cooling is integrated; ambient lab temperature control (18–22°C) and moderate run voltages (<200 V) are recommended to maintain thermal stability during extended runs.
