Beijing Liuyi DYY-4C High-Voltage Dual-Stable Electrophoresis Power Supply
| Brand | Beijing Liuyi (LY) |
|---|---|
| Origin | Beijing, China |
| Model | DYY-4C |
| Output Voltage Range | 5–1600 V (2 V resolution) |
| Output Current Range | 2–100 mA (1 mA resolution) |
| Max Power Output | 160 W |
| Dimensions (W × D × H) | 315 × 290 × 128 mm |
| Weight | 5 kg |
Overview
The Beijing Liuyi DYY-4C High-Voltage Dual-Stable Electrophoresis Power Supply is an engineered DC power source specifically designed for controlled electrophoretic separation in analytical and preparative molecular biology laboratories. It operates on a dual-stable regulation principle—maintaining precise, independent stability in both high-voltage and low-current regimes—enabling consistent electric field delivery across diverse gel and capillary electrophoresis configurations. Unlike conventional single-mode supplies, the DYY-4C dynamically sustains output integrity under variable load conditions typical of slab-gel, SDS-PAGE, native PAGE, and isoelectric focusing (IEF) applications. Its switching power supply architecture ensures minimal ripple (<0.5% RMS), thermal stability over extended run times, and efficient energy conversion—critical for reproducible migration kinetics and band resolution. The unit is intended for integration with standard horizontal and vertical electrophoresis chambers, including those compliant with ASTM D4294 or ISO 10576-1 for analytical electrophoretic systems.
Key Features
- Dual-stable output control: Maintains independent voltage and current stability thresholds to accommodate both high-field (e.g., rapid DNA fragment separation) and low-field (e.g., high-resolution protein isoform analysis) electrophoretic protocols
- Microprocessor-based digital regulation: Enables real-time parameter adjustment without interrupting operation; supports fine-tuning of voltage (5–1600 V, 2 V step resolution) and current (2–100 mA, 1 mA step resolution)
- Simultaneous LCD display: Shows voltage, current, elapsed time, and active mode (HV/LV) on a single monochrome screen—eliminating need for external multimeters or manual logging
- Two parallel output channels: Allows concurrent operation of two electrophoresis units or independent control of cathode/anode circuits in multi-chamber setups
- Comprehensive protection suite: Integrated hardware-level safeguards against overvoltage, overcurrent, overload, sudden load variation, and open-circuit (no-load) conditions—preventing arcing, gel overheating, or buffer electrolysis
- Non-volatile memory function: Stores up to 10 user-defined protocols—including voltage ramp profiles and timed shutdown sequences—for repeatable method execution across shifts or operators
- Compact benchtop form factor (315 × 290 × 128 mm) and lightweight design (5 kg): Optimized for space-constrained labs while maintaining robust aluminum chassis and forced-air cooling
Sample Compatibility & Compliance
The DYY-4C is compatible with standard electrophoresis media including polyacrylamide gels (4–20%, gradient or uniform), agarose gels (0.5–3.0%), and immobilized pH gradient (IPG) strips used in 2D electrophoresis. It meets electrical safety requirements per IEC 61010-1:2010 (Measurement, Control, and Laboratory Equipment – Safety Requirements) and conforms to electromagnetic compatibility standards EN 61326-1:2013. While not certified for clinical diagnostics under FDA 21 CFR Part 820 or ISO 13485, its parameter repeatability (±1.5% full scale over 8-hour continuous operation) supports GLP-aligned research workflows. Users performing QC testing in regulated environments are advised to implement periodic external calibration using NIST-traceable voltage/current standards.
Software & Data Management
The DYY-4C operates as a standalone instrument with no proprietary software dependency. All operational parameters are set and monitored locally via front-panel controls and LCD interface. Timer functionality supports preset run durations (up to 99 hr 59 min), with automatic power cutoff upon completion. For laboratories requiring electronic audit trails, the unit’s memory recall function enables traceability of applied settings per experiment—supporting internal documentation practices aligned with ISO/IEC 17025 clause 7.7 (Control of Equipment). Data export is not natively supported; however, analog output ports (0–5 V scaling) can be interfaced with third-party DAQ systems for time-synchronized logging of voltage/current waveforms.
Applications
- Vertical SDS-PAGE for molecular weight determination of denatured proteins (e.g., western blot sample prep)
- Horizontal agarose gel electrophoresis for plasmid DNA sizing, PCR product verification, and restriction digest analysis
- Native PAGE of enzyme complexes and oligomeric proteins under non-denaturing conditions
- Preparative electroelution of protein bands from polyacrylamide gels for downstream MS analysis
- Capillary zone electrophoresis (CZE) system support where stable high-voltage DC bias is required
- Teaching laboratories: Robust design and intuitive interface facilitate student training in fundamental electrophoretic principles per AP Biology and undergraduate biochemistry curricula
FAQ
What electrophoresis chamber models are compatible with the DYY-4C?
The DYY-4C supports all standard electrophoresis tanks accepting 0–1600 V DC input and ≤100 mA load, including common vertical systems (e.g., Bio-Rad Mini-PROTEAN, C.B.S. Scientific VE-180) and horizontal units (e.g., Thermo Fisher Owl B2, Convergent Bioscience HE-120). Verify chamber insulation rating exceeds 2 kV before use.
Does the DYY-4C support constant-power or constant-current modes?
No—it operates exclusively in constant-voltage or constant-current regulation, selected manually via front-panel mode toggle. It does not feature automatic power (wattage) regulation.
Can the DYY-4C be used for pulse-field gel electrophoresis (PFGE)?
No. PFGE requires programmable polarity reversal and asymmetric voltage pulses; the DYY-4C provides only unidirectional, steady-state DC output.
Is external calibration required, and how often?
Yes. For traceable accuracy, annual calibration against certified reference standards is recommended. Internal self-diagnostics verify basic regulator functionality but do not replace metrological calibration.
What maintenance is necessary for long-term reliability?
Clean ventilation grilles quarterly with compressed air; inspect HV cables annually for dielectric cracking; store in low-humidity environment (<60% RH) when not in use. Avoid operation above 40°C ambient temperature.
