BIO-DL Bio-1 Manual Single-Channel Pipette
| Brand | BIO-DL |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | Bio-1 |
| Instrument Type | Single-Channel Manual Pipette |
| Automation Level | Manual |
| Channels | 1 |
| Volume Range | 0.1–3 µL (primary precision range), extendable to 10 mL via interchangeable modules |
| Accuracy | ±3.00% at nominal volume |
| Display | 4-digit LCD |
| Tip Ejection | Roller-based mechanical ejection |
| Tip Attachment | Adjustable conical tip cone with tactile feedback |
| Chemical Resistance | High-grade polypropylene and PTFE-coated internal components |
| UV Stability | UV-stabilized polymer housing |
Overview
The BIO-DL Bio-1 Manual Single-Channel Pipette is an ergonomically engineered precision liquid handling instrument designed for high-reproducibility volumetric transfer in analytical laboratories, QC environments, and molecular biology workflows. It operates on the air displacement principle—where piston-driven air compression creates a vacuum to aspirate and dispense liquid—ensuring consistent performance across low-volume applications down to 0.1 µL. Unlike electronic pipettes, the Bio-1 relies on fully mechanical actuation, eliminating battery dependency and reducing calibration drift associated with motorized systems. Its design prioritizes tactile control, minimal hand fatigue, and long-term dimensional stability under repeated use. The instrument is calibrated and verified per ISO 8655-2:2022 (Piston-operated volumetric apparatus — Part 2: Piston pipettes) and supports traceable adjustment using certified reference standards.
Key Features
- Ultra-low volume capability: Optimized for accurate dispensing in the 0.1–3 µL range—a critical window for PCR master mix preparation, NGS library normalization, and microfluidic assay setup.
- Four-digit LCD display: Provides real-time volume readout with 0.1 µL resolution in the sub-10 µL range, enhancing operator confidence during fine-tuning.
- Roller-based tip ejection mechanism: Reduces thumb force by up to 40% compared to conventional spring-loaded ejectors, minimizing repetitive strain injury (RSI) risk during high-throughput operations.
- Adjustable conical tip cone: Accommodates variable tip taper tolerances (ISO 8655-compliant and non-certified tips), ensuring leak-free seals across multiple tip brands without manual torque adjustment.
- Chemical-resistant construction: Internal piston and seal materials include fluoropolymer-coated stainless steel and UV-stabilized thermoplastics, enabling compatibility with ethanol, DMSO, acetonitrile, and diluted acids/bases (pH 2–12).
- Modular volume extension: Interchangeable lower assemblies allow validated operation up to 10 mL (with appropriate large-volume tip adapters), maintaining accuracy within ±3.00% per ISO 8655-6 requirements.
Sample Compatibility & Compliance
The Bio-1 demonstrates robust performance with aqueous buffers, glycerol-containing solutions (up to 40% v/v), and low-surface-tension liquids (e.g., chloroform, isopropanol) when used with low-retention or filtered tips. It complies with ISO 8655-1 through -6 for metrological verification, including gravimetric testing protocols. While not intrinsically compliant with FDA 21 CFR Part 11 (as it lacks audit-trail-capable electronics), its mechanical architecture supports GLP/GMP environments where manual logbook documentation of calibration, maintenance, and usage is standard practice. All calibration certificates issued by authorized service centers include uncertainty budgets per ISO/IEC 17025:2017.
Software & Data Management
As a manually operated device, the Bio-1 does not integrate with laboratory information management systems (LIMS) or require firmware updates. However, its calibration records—including date, technician ID, reference standard ID, measured deviation, and environmental conditions (temperature, humidity)—are structured to align with Annex 11 (EU GMP) data integrity expectations when entered into controlled electronic spreadsheets or paper-based master equipment logs. Optional downloadable calibration templates (Excel/CSV) are available from BIO-DL’s global support portal for standardized recordkeeping.
Applications
- Quantitative preparation of qPCR and digital PCR reaction mixes requiring nanoliter-level precision.
- Serial dilution workflows in ELISA and cell-based assay development where coefficient of variation (CV) < 2.5% is mandated.
- Transfer of viscous samples (e.g., RNA extraction eluates, protein lysates) using reverse pipetting mode enabled by adjustable plunger travel.
- Quality control of raw materials in pharmaceutical manufacturing, aligned with USP Analytical Instrument Qualification guidelines.
- Educational laboratory instruction on volumetric technique fundamentals, error sources, and metrological traceability.
FAQ
Is the Bio-1 suitable for handling corrosive solvents such as concentrated HCl or NaOH?
The Bio-1 is rated for intermittent exposure to diluted acidic and basic solutions (pH 2–12); however, prolonged contact with >1 M HCl or >1 M NaOH is not recommended. For aggressive solvents, use chemically resistant tip types and perform post-use cleaning with deionized water followed by air-drying.
Does the Bio-1 require annual recalibration?
Yes—per ISO 8655-6 and most internal QA policies, manual pipettes should undergo full metrological verification at least once per year, or after any impact event, disassembly, or observed performance deviation.
Can the Bio-1 be sterilized in an autoclave?
No. The Bio-1 is not autoclavable due to thermal expansion mismatches between polymer and metal components. Surface disinfection with 70% ethanol or isopropanol is approved; avoid chlorine-based disinfectants.
What traceability documentation accompanies each unit?
Each Bio-1 ships with a factory calibration certificate referencing NIST-traceable mass standards, indicating tested volumes, deviations, and environmental conditions at time of verification.
