Boxun BSD-YX(F)3600 Large-Capacity Vertical Temperature-Controlled Orbital/Reciprocating Shaker

Overview

The Boxun BSD-YX(F)3600 is a dual-mode, large-capacity vertical shaker engineered for precision-controlled incubation and agitation in life science laboratories. It supports both orbital and reciprocating motion modes—enabling flexible adaptation to diverse biological workflows including microbial culture expansion, enzyme kinetics assays, hybridization protocols, and suspension-based cell line maintenance. Its core thermal regulation system employs PID-controlled forced-air convection with high-resolution digital feedback (±0.1°C setpoint resolution), ensuring stable temperature distribution across the entire working chamber (±1°C uniformity at 25°C, empty). The unit integrates a robust mechanical architecture—including imported precision bearings, brushless DC motor drive, and reinforced double-layer steel frame—to sustain continuous operation under full load while minimizing vibration transmission and acoustic noise. Designed for GxP-aligned environments, the shaker meets fundamental requirements for traceability, reproducibility, and operational integrity in regulated R&D and QC settings.

Key Features

• Dual-mode oscillation: Selectable orbital (Φ35 mm amplitude, 30–300 rpm) or reciprocating (0–50 mm amplitude, 30–250 rpm) motion via intuitive interface—optimized for E. coli growth, mammalian suspension cultures, or immunoassay plate mixing.
• Two independently configurable stainless-steel shelves (970×560 mm and 950×590 mm), each supporting up to 164 × 50 mL flasks or 36 × 1 L bottles—maximizing throughput without compromising thermal homogeneity.
• Programmable multi-segment control: Up to 10-step time-temperature-speed profiles stored internally; timing range extends to 999 hours with auto-termination and audible/visual alarm upon completion.
• Intelligent safety architecture: Independent overtemperature cutoff, motor overheat protection, door interlock switch, and dedicated limit thermostat ensure fail-safe operation during unattended runs.
• Brushless DC motor with constant-torque output eliminates carbon brush wear and delivers smooth acceleration/deceleration—critical for shear-sensitive samples such as primary hepatocytes or fragile protein complexes.
• Electrostatically coated exterior with mirror-finish 316L stainless-steel interior; rounded chamber corners and tempered glass observation window facilitate cleaning and sterility validation per ISO 14644 and cGMP Annex 1 guidelines.

Sample Compatibility & Compliance

The BSD-YX(F)3600 accommodates standard laboratory vessels—from 50 mL centrifuge tubes to 1 L baffled flasks—across both shelves simultaneously. Its wide amplitude and frequency ranges support low-shear bacterial propagation (e.g., E. coli BL21(DE3) at 200 rpm, 37°C) as well as high-aeration fungal fermentation (e.g., Aspergillus niger at 250 rpm, 30°C). The unit complies with IEC 61000-6-3 (EMC emission standards) and IEC 61010-1 (safety requirements for lab equipment). While not certified to FDA 21 CFR Part 11 out-of-the-box, its encrypted parameter lock, audit trail–ready event logging (when paired with optional printer or PC software), and deterministic control logic make it suitable for GLP/GMP environments where user-defined SOPs govern data integrity practices.

Software & Data Management

The built-in microprocessor controller features password-protected parameter locking, real-time display of actual vs. set values (temperature, speed, time), and non-volatile memory retention during power interruption. Optional accessories extend functionality: an integrated thermal printer enables hard-copy recording of temperature and speed profiles; GPRS-enabled remote alarm modules transmit fault alerts (e.g., temperature deviation >±2°C, door open >30 s) via SMS; and USB-connected PC software provides exportable CSV logs, trend visualization, and batch report generation. All digital outputs conform to ASTM E2500-13 (verification of computerized systems) foundational principles when deployed within validated workflows.

Applications

• High-density microbial cultivation for bioprocess development and seed train expansion
• Antibody production in CHO-S suspension cultures requiring consistent oxygen transfer
• DNA hybridization and Northern/Southern blotting with controlled agitation and thermal ramping
• Enzymatic digestion optimization (e.g., restriction endonuclease reactions at 37°C ±0.2°C)
• Environmental microbiology studies involving soil slurry enrichment under aerobic conditions
• Pharmaceutical stability testing per ICH Q1A(R2) guidelines using programmable temperature cycling

FAQ

Is the BSD-YX(F)3600 compliant with FDA 21 CFR Part 11?
The base unit does not include electronic signature or audit trail capabilities by default. However, when used with the optional PC monitoring software and configured with role-based access controls, timestamped logs, and change history tracking, it can be incorporated into a Part 11–compliant system under site-specific validation.
Can both shelves operate at different temperatures or speeds?
No—the unit maintains uniform chamber-wide temperature and synchronized oscillation across both shelves. Independent control per shelf is not supported.
What is the maximum flask size supported on the reciprocating mode?
The reciprocating shelf (950×590 mm) accommodates up to 40 × 1 L flasks or 64 × 750 mL flasks when using standard clamping fixtures.
Does the shaker support inert gas purging?
Yes—an optional inlet port allows connection to nitrogen or argon supply lines, enabling anaerobic or oxygen-sensitive applications such as strict anaerobe culture or redox-sensitive protein refolding.
How is calibration verified?
Users may perform field verification using NIST-traceable PT100 probes and tachometers. Factory calibration certificates (temperature and speed) are available upon request and align with ISO/IEC 17025–accredited procedures.