Chemagic Prepito-D Automated Nucleic Acid Extractor by Revvity

Overview

The Chemagic Prepito-D Automated Nucleic Acid Extractor is a compact, benchtop nucleic acid purification system engineered by Revvity (formerly PerkinElmer’s Life Sciences division) for high-integrity, walk-away sample processing in clinical diagnostics, translational research, and molecular pathology laboratories. It employs magnetic bead-based solid-phase extraction technology—leveraging paramagnetic particles functionalized with silica or carboxyl groups—to selectively bind nucleic acids under controlled chaotropic salt and pH conditions. Following binding, contaminants are removed via sequential wash steps with ethanol-based buffers, and purified DNA or RNA is eluted in low-ionic-strength buffer (e.g., TE or nuclease-free water). The system integrates thermally regulated incubation, precise magnetic separation, and programmable aspiration/dispensing to ensure consistent recovery across diverse biospecimens without manual intervention.

Key Features

• Benchtop footprint (28 × 39 × 34 cm) optimized for space-constrained BSL-2 labs and point-of-care testing environments.
• Dedicated fully automated workflow: single-touch start enables unattended operation from lysis through elution—no pipetting, tube transfers, or centrifugation required.
• Flexible batch processing: accommodates 1–12 samples per run using standardized 1.5 mL or 2.0 mL deep-well plates; no fixed cartridge constraints.
• Onboard reagent management: integrated positive-displacement liquid handler dispenses lysis, binding, wash, and elution reagents with ±2 µL accuracy at volumes ranging from 20–200 µL.
• Thermal control module maintains precise incubation temperatures (37°C–70°C) during lysis and binding steps to maximize yield from challenging matrices such as whole blood or fibrous tissue.
• Real-time status monitoring via intuitive touchscreen interface with event logging, error alerts, and protocol progress visualization.

Sample Compatibility & Compliance

The Prepito-D demonstrates validated performance across clinically relevant specimen types including EDTA-anticoagulated whole blood, citrated plasma, viral transport medium (VTM)-preserved nasopharyngeal swabs, stabilized saliva, and mechanically homogenized soft tissues. All protocols are developed in alignment with ISO 20387:2018 (Biobanking—General requirements for biobanking), and the instrument supports traceability features required under CLIA and CAP-accredited workflows. While not FDA 510(k)-cleared for IVD use in the U.S., it complies with IEC 61010-1 safety standards and carries CE marking for in vitro diagnostic use in the EU. The NMPA filing (GuoXieBei 20140357) confirms regulatory acceptance for clinical laboratory use in China under Class II device classification.

Software & Data Management

Operation is governed by embedded firmware with preloaded, locked-down protocols—including CE-IVD–validated kits for SARS-CoV-2 RNA extraction and human genomic DNA isolation. User-defined method editing is restricted to parameter tuning (e.g., incubation time, magnet dwell duration) within validated ranges to preserve assay integrity. Audit trails record operator ID, timestamp, protocol version, and critical events (e.g., lid open, reagent low alert) in accordance with GLP/GMP principles. Data export is supported via USB 2.0 to CSV or PDF report formats; no network connectivity or cloud integration is provided—ensuring data sovereignty and compliance with HIPAA and GDPR data residency requirements.

Applications

• High-fidelity nucleic acid isolation for downstream qPCR, digital PCR, and next-generation sequencing library preparation.
• Routine extraction in hospital microbiology labs supporting rapid pathogen detection workflows.
• Biobank preprocessing of longitudinal cohort samples where reproducibility and minimal hands-on time are critical.
• Research applications requiring parallel processing of heterogeneous sample types—e.g., matched tumor/normal tissue pairs with corresponding plasma cfDNA.
• Emergency response settings: rapid deployment for outbreak-associated nucleic acid purification with minimal infrastructure dependency.

FAQ

Is the Chemagic Prepito-D compatible with third-party magnetic bead kits?
No—the system is designed exclusively for use with Revvity Chemagic consumables and chemistries to guarantee binding efficiency, wash stringency, and eluate purity. Cross-kit usage voids performance validation and regulatory compliance.
What maintenance is required for long-term reliability?
Daily decontamination of the magnetic head and fluidics path using 70% ethanol; quarterly calibration of the liquid handler using gravimetric verification; annual preventive maintenance by Revvity-certified field service engineers.
Does the instrument support barcode scanning for sample tracking?
Yes—integrated 2D barcode reader supports GS1-compliant sample tube and plate labels, automatically populating run logs and linking extracted nucleic acids to source identifiers.
Can extraction protocols be exported or shared between instruments?
Protocol configurations are stored locally and cannot be transferred externally; however, Revvity provides centralized method distribution via secure firmware update packages issued through authorized service channels.
What is the typical nucleic acid yield and purity from 200 µL of whole blood?
Using the standard Chemagic DNA Blood Kit, mean yield is 3.2 µg ± 0.4 µg of genomic DNA with A260/A280 ≥ 1.80 and A260/A230 ≥ 2.0, as verified per ISO/IEC 17025-accredited QC testing.