CZGY HNY-100B Benchtop Air-Bath Constant-Temperature Orbital Shaker

Overview

The CZGY HNY-100B Benchtop Air-Bath Constant-Temperature Orbital Shaker integrates precise temperature regulation with controlled orbital agitation in a single compact platform. Engineered for reproducible incubation and mixing of biological samples, it operates on the principle of forced-air convection heating combined with sinusoidal orbital motion—ideal for applications requiring gentle yet uniform suspension of cells, microbes, or biochemical reagents. Unlike water-bath shakers, the air-bath design eliminates fluid contamination risks and simplifies maintenance while delivering rapid thermal response and high spatial uniformity across the working chamber. Its microprocessor-driven PID control architecture ensures stable setpoint adherence under variable load conditions, making it suitable for long-duration experiments such as microbial growth kinetics, enzyme assays, hybridization protocols, and cell culture expansion where both thermal consistency and mechanical reproducibility are critical.

Key Features

• High-fidelity orbital drive system featuring a brushless DC servo motor with bidirectional programmability—enabling forward, reverse, or alternating rotation sequences to optimize suspension homogeneity.
• PID-controlled air-heating module with mirror-polished stainless-steel chamber walls and optimized airflow ducting, achieving ±0.5°C temperature uniformity across the full 450 × 410 mm platform at 37°C.
• Multi-stage programmable operation: Users may define up to 9 independent segments combining discrete temperature setpoints, rotational speeds, and dwell durations—automatically executed without manual intervention.
• Real-time data acquisition via integrated USB interface: Continuously logs temperature, speed, and elapsed time; exports timestamped CSV files compatible with Excel, MATLAB, or LIMS platforms.
• Intelligent safety architecture including motor overheat shutdown, upper/lower temperature limit alarms with audible/visual alerts, door-open auto-stop, and power-loss recovery that resumes pre-interruption parameters.
• Slow-start acceleration profile minimizes splashing during initiation—critical for quantitative liquid handling in Erlenmeyer flasks or deep-well plates.
• LCD display with simultaneous three-parameter readout (°C, rpm, min/h), intuitive menu navigation, and persistent parameter memory—even after power cycling.

Sample Compatibility & Compliance

The HNY-100B accommodates standard laboratory vessels including 250 mL Erlenmeyer flasks (15-position configuration provided), test tubes, Petri dishes, and multi-well plates mounted on its single 450 × 410 mm platform. Its 80 L internal volume supports scalable workflows without compromising thermal stability. The instrument complies with general-purpose laboratory safety standards (IEC 61010-1) and is designed for use in environments adhering to Good Laboratory Practice (GLP) frameworks. While not certified for GMP manufacturing, its data logging, audit-trail-capable USB export, and parameter lockout functionality support traceability requirements in regulated academic and industrial research settings—including those referencing ISO/IEC 17025 or CLIA guidelines. No refrigeration capability is included; operation is limited to heating-only mode within ambient +5°C to 65°C.

Software & Data Management

No proprietary software installation is required. All data is stored directly onto standard USB flash drives in plain-text CSV format, ensuring compatibility with institutional IT infrastructure and eliminating vendor lock-in. Each logged file contains millisecond-resolution timestamps, enabling post-hoc correlation of thermal drift with kinetic response curves. Graphical replay is achievable using common spreadsheet tools: users can generate overlay plots of temperature vs. time, speed vs. time, or dual-axis visualizations to assess coupling effects. The system supports time-synchronized multi-parameter trending—particularly valuable when optimizing fermentation conditions or validating thermal ramp profiles in stability studies. Exported datasets include instrument ID, operator ID field (user-editable), and automatic annotation of alarm events (e.g., “T-ALERT_HI”, “MOTOR_LOCK”) for root-cause analysis.

Applications

This shaker serves core functions across life science laboratories: aerobic bacterial cultivation (E. coli, Bacillus spp.), yeast propagation, mammalian suspension cell passaging, immunoassay plate washing, DNA hybridization, protein expression screening, and environmental microbiology (e.g., BOD testing). Its programmable bidirectional mode enhances particle resuspension efficiency in sedimentation-prone samples such as primary tissue digests or antibody-conjugated beads. In teaching labs, the intuitive interface and robust construction support undergraduate training in experimental design, error propagation analysis, and equipment validation protocols. Industrial QC labs utilize its repeatability for method transfer between R&D and production-scale bioreactor systems—especially where air-agitated benchtop models serve as surrogates for larger orbital incubators.

FAQ

Does the HNY-100B support cooling functionality?
No. It is a heating-only air-bath shaker with a minimum operating temperature of ambient +5°C.
Can I mount custom-sized platforms or clamps?
Yes—the 450 × 410 mm platform accepts third-party universal clamping kits compatible with standard M4/M6 threaded inserts.
Is USB data logging compliant with FDA 21 CFR Part 11?
The system provides raw audit-ready data but lacks electronic signature, role-based access control, or immutable storage—thus requiring supplemental procedural controls to meet Part 11 requirements.
What is the maximum flask size supported without instability?
Stable operation is verified for up to 2 L flasks when centered; however, optimal performance is achieved with vessels ≤500 mL distributed symmetrically.
How frequently should calibration verification be performed?
We recommend quarterly verification of temperature accuracy using an NIST-traceable thermometer and annual speed calibration with a tachometer per ISO/IEC 17025 Annex A.3 guidance.