CZGY SHY-2AHS Water Bath Constant-Temperature Constant-Speed Shaker

Overview

The CZGY SHY-2AHS Water Bath Constant-Temperature Constant-Speed Shaker is an engineered platform for precise, reproducible agitation and thermal control of liquid-phase biological and biochemical samples under submerged conditions. It operates on the principle of orbital or reciprocal motion within a temperature-regulated water bath, enabling uniform heat transfer and mechanical mixing without direct contact between heating elements and samples—critical for heat-sensitive cultures, enzyme assays, solubility studies, and hybridization protocols. Unlike air-jacketed shakers, the water bath design ensures superior thermal homogeneity (±0.5 °C across the chamber), minimal evaporation, and reduced thermal shock during prolonged incubation. The unit integrates digital speed and temperature feedback loops with independent PID control algorithms, supporting stable operation at any setpoint from ambient to 100 °C and across the full 60–300 rpm range.

Key Features

• Dual-mode oscillation: Selectable orbital (circular) or reciprocal (linear back-and-forth) motion via front-panel toggle—enabling method-specific optimization for cell suspension, immunoassay plate washing, or sediment resuspension.
• High-resolution digital interface: LED-displayed real-time values for temperature (°C), speed (rpm), and remaining timer (min), with intuitive push-button navigation and non-volatile parameter memory.
• Robust stainless-steel water bath chamber (304 grade) with integrated anti-corrosion heater and calibrated Pt100 sensor—designed for long-term stability in saline, buffered, or organic-aqueous media.
• Single-platform configuration (400 × 310 mm) accommodates standard flasks (up to 5 L), culture bottles, multi-well plates (via optional clamping adapters), and custom sample carriers.
• Extended programmable timer (0–9999 minutes, ~7 days) supports unattended overnight and multi-shift experiments—essential for growth curve analysis, kinetic binding studies, and batch processing workflows.
• Over-temperature and dry-run protection circuits automatically cut power if bath level falls below safe threshold or if temperature exceeds 105 °C—compliant with IEC 61010-1 safety standards for laboratory equipment.

Sample Compatibility & Compliance

The SHY-2AHS is validated for use with aqueous buffers (PBS, Tris-HCl), microbial growth media (LB, TB), cell culture supernatants, serum-containing formulations, and low-viscosity reagents (<50 mPa·s). It is not rated for volatile organic solvents (e.g., acetone, chloroform) or highly corrosive solutions (e.g., concentrated HNO₃, HF). The unit conforms to GB/T 29895–2013 (Chinese national standard for laboratory shakers) and meets electromagnetic compatibility requirements per GB/T 18268.1–2010 (equivalent to IEC 61326-1). While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, its digital logging capability (via optional RS232/USB output) supports traceable data capture in GLP-compliant environments when paired with validated third-party software.

Software & Data Management

The SHY-2AHS operates as a standalone instrument with no embedded operating system. However, its optional serial (RS232) or USB interface enables connection to host PCs running custom LabVIEW, Python (PySerial), or MATLAB scripts for remote parameter setting, real-time monitoring, and time-stamped data export (CSV format). All setpoints and runtime logs—including start/stop timestamps, average speed deviation, and thermal drift profiles—are retained in non-volatile memory for post-experiment audit. For regulated laboratories, integration with LIMS or ELN systems is achievable through middleware compliant with ASTM E1384 and ISO/IEC 17025 Annex A.2 data integrity guidelines.

Applications

• Microbial and mammalian cell culture: Maintaining planktonic growth in E. coli, CHO, or HEK293 suspensions under controlled shear and thermal conditions.
• Biochemical assay development: Facilitating antigen-antibody binding kinetics, protein denaturation studies, and ligand-receptor equilibration in buffer systems.
• Environmental and clinical sample preparation: Homogenizing wastewater sludge, blood plasma aliquots, or soil extracts prior to filtration or centrifugation.
• Pharmaceutical QC: Supporting dissolution testing of solid dosage forms per USP , extraction efficiency validation, and stability-indicating method development.
• Material science pre-treatment: Dispersing nanoparticles in aqueous colloids or hydrating hydrogels under isothermal agitation.

FAQ

Is the SHY-2AHS suitable for use with CO₂-enriched atmospheres?

No—the unit lacks gas-tight sealing or inlet/outlet ports; it is intended for ambient-air water bath operation only.
Can multiple platforms be installed?

No—this model features a fixed single-platform architecture; multi-tier configurations require the SHY-3 series.
What is the maximum recommended fill volume for the water bath?

The bath capacity is 18 L; optimal performance is achieved at 12–16 L to ensure full submersion of flask bases and avoid overflow during high-speed oscillation.
Does the device support external temperature probe input for sample-core monitoring?

Not natively—the built-in Pt100 sensor measures bath temperature only; external probe integration requires third-party signal conditioning hardware.
Is calibration documentation provided with shipment?

A factory-issued calibration certificate (covering temperature and speed verification at three points each) is available upon request at no additional cost—traceable to CNAS-accredited reference standards.