DLAB TopPette Single-Channel Adjustable-Volume Manual Pipette

Overview

The DLAB TopPette Single-Channel Adjustable-Volume Manual Pipette (Model: 10–100 µL) is an ISO 8655–certified precision liquid handling instrument engineered for reproducible volumetric delivery in routine and regulated laboratory environments. Utilizing a gas-tight air displacement principle, the pipette maintains consistent aspiration and dispensing performance across its full range through a calibrated spring-loaded piston mechanism housed in a chemically resistant, autoclavable lower assembly. Designed for daily use in QC/QA labs, clinical diagnostics, academic research, and pharmaceutical development, the TopPette series meets the mechanical and metrological requirements defined in ISO 8655-2 (volumetric performance), ISO 8655-5 (calibration), and supports compliance with GLP, GMP, and FDA 21 CFR Part 11 when integrated into validated workflows. Its lightweight construction (95 g), optimized plunger travel force (<1.2 N for full stroke), and tactile volume-setting feedback minimize operator-induced variability—critical for long-duration pipetting tasks.

Key Features

• Ergonomic full-hand grip with textured, non-slip surface contours aligned to natural hand anatomy—reduces muscular strain during extended use.
• Dual-scale volume display window with high-contrast numerals; rotation dial provides precise, click-stop incremental adjustment (1 µL steps) and integrated volume lock to prevent accidental changes.
• Modular lower assembly: fully detachable tip cone with PTFE-coated O-rings and stainless-steel piston shaft—resistant to common solvents including ethanol, acetone, and 10% sodium hypochlorite.
• Integrated filter barrier option (replaceable hydrophobic PTFE filter) prevents aerosol contamination of internal mechanisms and cross-sample carryover.
• Autoclavable at 121°C for 20 minutes (without filter); all critical wetted parts withstand repeated sterilization cycles without dimensional drift or seal degradation.
• Tool-assisted calibration compliant with ISO 8655-5: single-point adjustment via included hex key; traceable to NIST-traceable standards.
• Low-force ejection lever with optimized fulcrum geometry enables smooth, consistent tip ejection—even with high-retention or filtered tips.

Sample Compatibility & Compliance

The TopPette 10–100 µL pipette delivers accurate and repeatable transfers of aqueous buffers, cell suspensions, serum, DNA/RNA solutions, and low-viscosity organic mixtures (≤10 mPa·s). It is compatible with all major brands of universal-fit conical 300 µL pipette tips—including low-retention, filtered, and sterile variants. Performance validation data per ISO 8655-2 confirms accuracy within ±0.8% and coefficient of variation (CV) ≤0.15% at nominal volume (100 µL), and ≤1.2% / ≤0.6% at 10 µL. The device carries CE marking under Directive 2014/30/EU (EMC) and 2012/69/EU (LVD), and complies with RoHS 2011/65/EU. Documentation packages include Factory Calibration Certificate (traceable to national standards), Declaration of Conformity, and ISO 8655 test report.

Software & Data Management

As a manually operated instrument, the TopPette requires no embedded firmware or software interface. However, its design supports integration into digital lab ecosystems: calibration records, maintenance logs, and usage tracking can be managed via LIMS or ELN platforms (e.g., LabVantage, Benchling, Veeva Vault). Each unit bears a unique serial number engraved on the body for asset tracking. For laboratories subject to 21 CFR Part 11, the pipette’s mechanical calibration process—documented using controlled forms and signed by authorized personnel—fulfills audit trail requirements when paired with electronic record systems that enforce user authentication, version control, and electronic signatures.

Applications

• Preparation of standard curves and dilution series in ELISA, qPCR, and spectrophotometric assays.
• Cell culture passaging and reagent addition in adherent and suspension workflows.
• Sample preparation for HPLC, GC, and mass spectrometry analysis where sub-microliter precision is not required but inter-operator consistency is critical.
• Quality control testing in food safety, environmental monitoring, and raw material verification per ASTM E2754 and ISO/IEC 17025.
• Teaching laboratories requiring robust, serviceable instruments for undergraduate and graduate training in fundamental pipetting technique and metrology principles.

FAQ

Is the TopPette 10–100 µL pipette suitable for viscous or volatile liquids?
It is optimized for aqueous and low-viscosity liquids (≤10 mPa·s). For viscous samples (e.g., glycerol, serum), reverse pipetting technique is recommended; for highly volatile solvents, pre-wetting and reduced aspiration speed improve accuracy.
Can this pipette be calibrated in-house?
Yes—using the included hex key and gravimetric method per ISO 8655-5. Calibration must be performed with certified analytical balance (≥0.01 mg readability), distilled water at 20°C, and temperature-controlled environment (±1°C).
What is the recommended recalibration frequency?
Per CLSI EP15-A3 and ISO/IEC 17025, recalibrate every 3–6 months depending on usage intensity; immediately after impact, sterilization cycles exceeding 50, or observed performance deviation.
Does DLAB provide service documentation for audit readiness?
Yes—factory calibration certificate, ISO 8655 test report, CE DoC, and maintenance log templates are supplied digitally upon request and support GLP/GMP inspection readiness.
Are spare parts available globally?
Full-service kits—including O-rings, piston shafts, tip cones, and filter cartridges—are stocked by DLAB’s regional distributors in North America, EU, and APAC, with lead time ≤5 business days for standard orders.