DMT 110P Isolated Microvessel Pressure-Diameter Measurement System
| Origin | Denmark |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | 110P |
| Quotation | Upon Request |
| Vessel Diameter Range | >60 µm |
| Axial Alignment | Manual X/Y/Z Adjustment |
| Bath Volume | Up to 10 mL (3 mL Standard) |
| Bath Material | Acid-Resistant Stainless Steel |
| Tension Range | ±50 mN |
| Tension Resolution | 0.01 mN |
| Pressure Range | 0–250 mmHg |
| Pressure Resolution | 0.1 mmHg |
| Temperature Control | Built-in Electronic Feedback Heating |
| Temp Range | Ambient to 50 °C |
| Temp Accuracy | ±0.1 °C |
| Analog Output | 1.0 V F.S. (12-bit) |
| Digital Interface | RS232 / RS485 |
| Power Input | 100–240 VAC, Auto-Switching, 50/60 Hz |
| Environmental Operating Range | 15–30 °C |
| Optional Modules | Flowmeter (15–1500 µL/min), pH Meter (0–14, 0–50 °C), Peristaltic Pump (2.5–50 rpm) |
Overview
The DMT 110P Isolated Microvessel Pressure-Diameter Measurement System is a precision-engineered platform for quantitative assessment of structural and functional responses in isolated resistance-level microvessels under near-physiological conditions. Designed around the principles of intraluminal pressure myography, the system enables real-time, high-fidelity measurement of vessel diameter, wall tension, transmural pressure, shear stress, and vascular resistance—parameters essential for mechanistic studies of vascular tone regulation, endothelial function, smooth muscle contractility, and pharmacological modulation. The 110P configuration supports vessels with diameters exceeding 60 µm, making it suitable for mesenteric, cerebral, coronary, and renal arterioles commonly used in cardiovascular and metabolic disease research. Its modular architecture allows seamless integration with complementary modalities—including fluorescence microscopy for intracellular Ca2+ or pH imaging—enabling correlative structure-function analysis at the microvascular level.
Key Features
- Physiologically relevant pressurized myography: Maintains controlled intraluminal pressure (0–250 mmHg) and superfusion flow to preserve endothelial integrity and smooth muscle viability
- Dual-axis digital video caliper system: Fully digitized, software-based inner/outer diameter measurement with sub-pixel resolution and automated edge detection
- High-sensitivity force transduction: ±50 mN tension range with 0.01 mN resolution for longitudinal force monitoring during pressure-step protocols
- Integrated thermal control: Precision electronic feedback heating (ambient to 50 °C, ±0.1 °C stability) with external temperature probe input for bath-side validation
- Modular fluidics interface: Standardized inlet/outlet ports for gas exchange, drug perfusion, and waste drainage; compatible with optional peristaltic pumps (2.5–50 rpm), flowmeters (15–1500 µL/min), and calibrated pH electrodes (0–14, 0–50 °C)
- Comprehensive analog and digital I/O: 12-bit analog output (1.0 V full scale) for integration with third-party DAQ systems; RS232/RS485 serial interface for bidirectional communication and remote parameter control
Sample Compatibility & Compliance
The DMT 110P accommodates isolated microvessels ≥60 µm in native diameter, including first- to third-order arterioles dissected from rodent, porcine, or human tissue specimens. Vessels are cannulated on dual glass micropipettes and secured using low-toxicity cyanoacrylate adhesives or custom-fit O-rings. The stainless-steel single-chamber bath (max. 10 mL volume, 3 mL standard) resists corrosion from physiological saline, Krebs-Henseleit buffer, and common vasoactive agents—including H2O2, acetylcholine, phenylephrine, and sodium nitroprusside. All hardware components comply with IEC 61010-1 safety standards for laboratory electrical equipment. While the system itself does not carry FDA clearance or CE-IVD marking, its operational parameters and data acquisition protocols align with GLP-compliant experimental design requirements for preclinical vascular pharmacology studies referenced in ISO 10993-1 and ASTM F2129.
Software & Data Management
Control and analysis are performed via DMT’s proprietary MyoVIEW™ software suite (Windows 10/11 compatible), which provides synchronized acquisition of pressure, diameter, tension, temperature, and optional sensor inputs (e.g., pH, flow rate). Data are timestamped with millisecond precision and stored in HDF5 format—ensuring long-term archival integrity and interoperability with MATLAB, Python (h5py), and LabVIEW environments. Audit-trail functionality logs all user-initiated parameter changes, calibration events, and session metadata, supporting traceability in regulated research settings. Export options include CSV, Excel, and PDF report generation with customizable templates compliant with internal SOPs or journal submission guidelines (e.g., Microcirculation, Journal of Cerebral Blood Flow & Metabolism).
Applications
- Endothelium-dependent and -independent vasoreactivity profiling in hypertension, diabetes, and aging models
- Assessment of vascular remodeling following chronic angiotensin II infusion or salt-loading protocols
- Mechanistic evaluation of ion channel modulators (e.g., TRPV4, BKCa, KV) on myogenic tone
- Pharmacodynamic characterization of novel antihypertensive compounds in primary microvessels
- Correlative studies combining pressure-diameter curves with Fluo-4 AM calcium imaging or SNARF-1 pH sensing
- Validation of computational hemodynamic models using empirically derived pressure-radius relationships
FAQ
What vessel types and sizes are compatible with the DMT 110P?
The system is optimized for isolated microvessels with native diameters >60 µm, including mesenteric, cerebral, coronary, and renal resistance arteries. Larger conduit vessels may be studied using the 110PXL variant.
Is the system compatible with fluorescence microscopy setups?
Yes—the optical path is unobstructed, and the bath chamber features standardized mounting interfaces for upright or inverted microscopes. Synchronization of pressure/diameter data with image acquisition is supported via TTL triggers and software API.
Does the system support automated pressure step protocols?
Yes—MyoVIEW™ includes programmable pressure ramps, square-wave steps, and oscillatory waveforms with user-defined dwell times, enabling myogenic reactivity and passive distensibility assessments.
Can temperature be independently controlled in the lumen versus the bath?
No—the system maintains uniform temperature across the bath and lumen via conductive heating. For differential thermal challenges, external inline heaters or cooled perfusate reservoirs must be integrated upstream.
What regulatory documentation is provided for audit readiness?
A comprehensive Instrument Qualification Package (IQ/OQ) is available upon request, including factory calibration certificates, traceable pressure/tension verification reports, and software validation summaries aligned with ALCOA+ data integrity principles.
