DMT 111P Vascular Pressure-Diameter Measurement System
| Origin | Denmark |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Imported |
| Model | DMT 111P |
| Pricing | Upon Request |
Overview
The DMT 111P Vascular Pressure-Diameter Measurement System is a high-precision, ex vivo platform engineered for quantitative functional assessment of isolated microvessels under near-physiological conditions. Based on the principle of pressurized arteriography, the system enables real-time, video-based measurement of internal and external vessel diameters while independently controlling intraluminal pressure, circumferential wall tension, longitudinal stretch, bath temperature, and (optionally) luminal flow rate and extracellular calcium concentration. Designed specifically for vessels with diameters exceeding 60 µm—including resistance arteries, small mesenteric arteries, cerebral arterioles, and coronary microvessels—the DMT 111P supports mechanistic studies in vascular physiology, pharmacology, pathophysiology, and translational cardiovascular research. Its modular architecture integrates optical, mechanical, thermal, and fluidic control subsystems into a single synchronized acquisition environment, facilitating reproducible quantification of structural parameters such as wall thickness, lumen volume, cross-sectional area, and distensibility—key metrics in hypertension, diabetes, aging, and endothelial dysfunction models.
Key Features
- Real-time video microscopy via integrated inverted DMT microscope with bottom-mounted optical window for non-invasive, continuous internal/external diameter tracking
- Independent dual-pressure control: precise intraluminal pressure regulation (0–250 mmHg, ±0.1 mmHg accuracy) and transmural pressure gradient modulation
- High-resolution force measurement: ±50 mN tension range with 0.01 mN resolution, enabling quantification of myogenic tone and passive mechanical properties
- Electronic feedback heating system maintaining bath temperature from ambient to 50°C (±0.1°C stability), eliminating requirement for continuous perfusion-based thermal management
- Corrosion-resistant acid-proof stainless steel single-chamber bath (standard 3 mL, max 10 mL capacity) with integrated ports for drug addition, gas equilibration (O₂/CO₂/N₂), rapid fill/drain, and luminal perfusion
- Full hardware/software synchronization: all analog signals (pressure, tension, temperature, flow, pH) digitized and time-aligned with video frames at user-defined sampling rates
- Manual three-axis (X/Y/Z) micromanipulator for precise vessel cannulation and alignment using glass micropipettes
Sample Compatibility & Compliance
The DMT 111P accommodates isolated, cannulated microvessels ≥60 µm in native diameter—including but not limited to rat/mouse mesenteric, cerebral, renal, and coronary resistance arteries. Vessel viability is maintained for >24 hours under optimized physiological salt solution (PSS) conditions with continuous oxygenation and temperature control. The system conforms to GLP-aligned experimental practices: all hardware calibrations (pressure, tension, temperature) are traceable to NIST-certified standards; software supports audit trails, user access levels, and electronic signatures per FDA 21 CFR Part 11 requirements when configured with compliant data acquisition modules. Experimental protocols align with established guidelines from the American Heart Association (AHA) and International Society of Hypertension (ISH) for resistance artery myography.
Software & Data Management
The DMT 111P operates with proprietary VesselTrack™ software, providing real-time visualization of diameter traces synchronized with pressure, tension, temperature, and optional flow/pH signals. Calibration routines include semi-automatic weight-based tension calibration and manual liquid-column pressure calibration. Video analysis supports user-defined pixel-to-micron conversion with dynamic recalibration during long-term experiments. Raw data export is supported in ASCII (.txt), MATLAB (.mat), and CSV formats; time-series datasets retain full metadata (timestamp, hardware configuration, calibration history). Optional integration with LabChart or Spike2 enables advanced signal processing, frequency-domain analysis, and automated detection of myogenic responses or agonist-induced constriction/relaxation kinetics.
Applications
- Myogenic reactivity assessment in response to stepwise pressure elevation
- Endothelium-dependent and -independent vasodilation/constriction profiling (e.g., acetylcholine, sodium nitroprusside, phenylephrine)
- Structural remodeling quantification: wall-to-lumen ratio, media thickness, incremental distensibility
- Pharmacological characterization of ion channel modulators, RAS inhibitors, and vasoactive peptides
- Pathophysiological modeling: hypertensive, diabetic, septic, and aging-associated microvascular dysfunction
- Validation of novel therapeutic interventions targeting vascular stiffness, tone, or barrier integrity
FAQ
What vessel size range is supported by the DMT 111P?
The system is optimized for isolated microvessels with native diameters greater than 60 µm. Typical preparations include 80–300 µm resistance arteries harvested from rodent mesentery, brain, kidney, or heart.
Is continuous bath perfusion required?
No—integrated electronic feedback heating eliminates dependence on constant perfusion for thermal stability. However, optional peristaltic pump and flowmeter modules support controlled luminal or bath perfusion protocols.
Can the system measure both active and passive mechanical properties?
Yes. By combining pressure-diameter curves under Ca²⁺-free conditions (passive) and physiological Ca²⁺ (active), users can isolate myogenic tone, elastic modulus, and incremental compliance.
What calibration procedures are included?
Semi-automatic tension calibration using standard weights, manual pressure calibration via mercury manometer or digital reference gauge, and user-defined video pixel calibration with stage micrometer.
Does the system support ISO/IEC 17025 or GLP-compliant documentation?
When deployed with validated software configurations and documented calibration logs, the DMT 111P meets core instrumentation requirements for GLP audits; full ISO/IEC 17025 compliance depends on laboratory-specific validation protocols and metrological traceability implementation.
