Domel CENTRIC 250 Benchtop High-Speed Refrigerated Centrifuge

Overview

The Domel CENTRIC 250 is a precision-engineered benchtop high-speed refrigerated centrifuge designed for demanding separation tasks in life science, clinical diagnostics, and pharmaceutical laboratories. It operates on the principle of sedimentation under controlled gravitational force (RCF), enabling rapid and reproducible isolation of subcellular components—including organelles, nucleic acids, proteins, and viral particles—based on differences in density, size, and shape. With a maximum rotational speed of 16,200 rpm and a peak relative centrifugal force of 23,230 × g, the CENTRIC 250 meets the performance requirements for routine and advanced biomolecular applications such as plasmid DNA purification, exosome enrichment, and ribosome fractionation. Its integrated refrigeration system maintains sample integrity across extended runs by sustaining temperatures between –10°C and +40°C, with precise PID-controlled cooling independent of ambient conditions.

Key Features

• Brushless induction motor ensuring long service life, low maintenance, and consistent torque delivery across all speed ranges
• Electric lid locking mechanism with dual safety interlocks compliant with IEC 61010-2-020 for operational integrity and user protection
• High-resolution LED display with intuitive keypad navigation supporting timer programming (10 s – 99 min 50 s, or continuous mode) and real-time monitoring of speed, RCF, time, temperature, and acceleration/deceleration profiles
• 10 programmable acceleration and deceleration steps (including “no-brake” option) to minimize sample disturbance during pelleting and resuspension phases
• Quiet operation at < 62 dB(A) achieved through optimized rotor dynamics, balanced chamber design, and vibration-dampening base construction
• Compact footprint (248 × 300 × 414 mm) suitable for standard laboratory benches without compromising thermal stability or mechanical rigidity
• Comprehensive rotor library—including fixed-angle, swing-out, PCR strip, hematocrit, and cell culture rotors—with forward-compatible adapter support for tubes ranging from 0.2 mL PCR strips to 50 mL conical tubes

Sample Compatibility & Compliance

The CENTRIC 250 supports a broad spectrum of biological and biochemical samples, including whole blood, cell lysates, clarified supernatants, and viscous reagents. All rotors are certified to ISO 15195:2018 (calibration of centrifuges) and manufactured to EN 61000-6-3 (EMC emissions) and EN 61000-6-2 (EMC immunity) standards. The unit conforms to IEC 61010-1 (safety requirements for electrical equipment) and carries CE marking for use within the European Economic Area. For regulated environments, its programmable method storage, audit-ready run logs, and non-volatile memory align with GLP and GMP documentation expectations; while not inherently 21 CFR Part 11-compliant, it may be integrated into validated workflows via external LIMS or electronic lab notebook systems.

Software & Data Management

The CENTRIC 250 stores up to 100 user-defined protocols with full parameter recall—including setpoint speed, temperature, time, acceleration/deceleration ramping, and brake selection. Each run generates a timestamped log containing actual vs. target values, error codes (e.g., imbalance detection, overtemperature alerts), and rotor-specific calibration identifiers. Data export is supported via USB interface (optional accessory) for integration into laboratory information management systems. Firmware updates are delivered via secure download channels, maintaining traceability per ISO/IEC 17025 clause 5.9.2 for instrument software validation.

Applications

• Purification of genomic DNA, RNA, and plasmids from bacterial, mammalian, and plant extracts
• Isolation of extracellular vesicles (exosomes, microvesicles) using differential ultracentrifugation protocols
• Preparative separation of mitochondria, nuclei, and lysosomal fractions from tissue homogenates
• Clinical processing of serum, plasma, and PBMCs in diagnostic and biobanking workflows
• High-throughput centrifugation of 96-well plates (with compatible swing-bucket rotor) for ELISA and immunoassay preparation
• Cell pellet harvesting in transient transfection and CRISPR editing workflows requiring gentle yet efficient recovery

FAQ

What is the minimum and maximum operating temperature range?
The refrigeration system maintains chamber temperatures from –10°C to +40°C, adjustable in 1°C increments.
Can the CENTRIC 250 accommodate 15 mL conical tubes?
Yes—using the RA 8/15 fixed-angle rotor (max 6,000 rpm / 4,110 × g) or RS 6/12 swing-bucket rotor (max 4,400 rpm / 2,580 × g), both compatible with standard polypropylene 15 mL tubes.
Is rotor imbalance detection automatic?
Yes—the system continuously monitors vibration signatures during acceleration and deceleration and halts operation if imbalance exceeds safety thresholds defined per ISO 15195 Annex C.
Does the unit support remote monitoring or network connectivity?
No native Ethernet or Wi-Fi interface is included; however, USB-based data transfer and third-party API integration via serial emulation are feasible with appropriate middleware.
Are rotor calibrations traceable to national standards?
All factory-calibrated rotors include individual certificates referencing NIST-traceable tachometer and temperature sensor verification, valid for two years under normal usage conditions.