Dongxi Analysis LC-5510 High-Performance Liquid Chromatograph

Overview

The Dongxi Analysis LC-5510 High-Performance Liquid Chromatograph is a modular, fully integrated HPLC system engineered for precision, reproducibility, and operational robustness in routine and method-development laboratories. Based on classical reversed-phase, normal-phase, and ion-exchange chromatographic principles, the LC-5510 delivers reliable separation performance across diverse analyte classes—including small-molecule pharmaceuticals, organic acids, amino acids, nucleotides, and natural product extracts. Its architecture conforms to widely accepted HPLC design conventions: dual high-pressure reciprocating piston pumps with active pulse damping, a thermostatically controlled column compartment with real-time temperature monitoring and safety cutoff, a six-port manual or automated injection valve compliant with ISO 5725-2 repeatability standards, and a high-stability UV-Vis absorbance detector employing holographic concave grating optics. Designed and manufactured in Beijing, the system meets essential mechanical and electrical safety requirements per GB/T 18268.1–2010 (equivalent to IEC 61326-1), and supports method validation workflows aligned with ICH Q2(R2) and USP .

Key Features

• Modular hardware architecture enabling field-upgradable configurations—pump modules, detector options, and autosampler integration follow standardized mechanical and electrical interfaces.
• Dual-plunger high-pressure pump with proprietary active pulse dampening technology; achieves flow accuracy ≤ ±0.2% RSD and pressure stability ≤ ±0.5% RSD at 1 mL/min, 30 MPa.
• UV-Vis detector featuring a holographic concave grating optical path, mercury lamp-based wavelength calibration traceable to NIST SRM 193b, and programmable multi-wavelength time-segmented acquisition (up to 8 wavelengths per run).
• Column oven with Peltier-based cooling and heating, maintaining temperature uniformity ±0.3 °C across the full 5–80 °C range; includes audible/visual over-temperature alarm and automatic power cutoff at ≥85 °C.
• 120-position autosampler with low-dead-volume syringe loop injection (0.1–100 µL), precision motorized positioning, and sealed vial tray to minimize solvent evaporation during long sequences.
• Full PC-based control via Dongxi ChromaLink™ software (v3.2+), supporting method building, sequence scheduling, real-time chromatogram display, peak integration, and audit-trail-enabled data archiving compliant with FDA 21 CFR Part 11 requirements.

Sample Compatibility & Compliance

The LC-5510 accommodates standard 3.9–4.6 mm ID analytical columns (up to 250 mm length) and microbore formats (1–2 mm ID). It supports mobile phases containing aqueous buffers (e.g., phosphate, acetate), organic modifiers (acetonitrile, methanol), and additives such as trifluoroacetic acid or ammonium formate—compatible with stainless steel and PEEK fluidic paths rated to 42 MPa. The system complies with ISO/IEC 17025:2017 general requirements for testing laboratories when operated under documented SOPs. All electronic records generated by ChromaLink™ software include timestamped user authentication, electronic signatures, and immutable audit trails—facilitating GLP/GMP audits and regulatory submissions per EMA CHMP/Q5C and FDA guidance on analytical procedure validation.

Software & Data Management

ChromaLink™ is a Windows-based chromatography data system (CDS) developed in accordance with ASTM E2524–22 for chromatographic software validation. It provides full instrument control, real-time signal visualization, baseline correction algorithms (linear, exponential, polynomial), peak detection using derivative threshold and valley-to-valley methods, and customizable report templates exportable to PDF, CSV, and XML. Raw data files (.dxcd) are stored with embedded metadata (instrument ID, method parameters, operator ID, acquisition timestamp), and support version-controlled method libraries. Data integrity safeguards include role-based access control (administrator, analyst, reviewer), automatic backup to network drives, and optional integration with LIMS via ASTM E1467-compliant API.

Applications

The LC-5510 is routinely deployed in quality control laboratories for assay quantification of active pharmaceutical ingredients (APIs) per USP monographs; in environmental labs for PAH and pesticide residue analysis per EPA Method 8330B; in food safety testing for mycotoxin screening (aflatoxins, ochratoxin A); and in academic research for metabolite profiling in plant and microbial extracts. Its wide linear dynamic range (0.005–2.0 AUFS), low noise (<±0.25 × 10⁻⁵ AU), and compatibility with gradient elution make it suitable for both isocratic QC release testing and complex multi-step method development.

FAQ

Is the LC-5510 compatible with third-party CDS platforms such as Chromeleon or Empower?
Yes—via ASCII-based ASCII/CSV export and optional RS-232 or Ethernet communication modules supporting industry-standard SCPI command sets.
Does the UV-Vis detector support post-run spectral deconvolution?
No—the detector performs single-wavelength or time-programmed multi-wavelength acquisition only; full-spectrum scanning requires optional diode-array upgrade (LC-5510-DAD variant).
What maintenance intervals are recommended for the high-pressure pump seals?
With standard acetonitrile/water mobile phases, seal replacement is advised every 6,000–8,000 operating hours or upon observed pressure fluctuation >2% RSD over 30 minutes.
Can the column oven accommodate guard columns and in-line filters?
Yes—the chamber includes dedicated mounting brackets for 2–4 mm ID guard cartridges and 0.2–0.45 µm in-line filters without compromising thermal homogeneity.
Is remote monitoring supported?
Yes—ChromaLink™ supports secure remote desktop access (via TLS-encrypted RDP) and real-time status dashboards viewable through web browser on local intranet.