DRETOP XC3-35RH Stackable Temperature- and Humidity-Controlled Orbital Shaker

Overview

The DRETOP XC3-35RH Stackable Temperature- and Humidity-Controlled Orbital Shaker is an integrated laboratory platform engineered for precision-controlled cultivation, suspension culture, and biochemical reaction kinetics under dynamically stabilized environmental conditions. Unlike conventional shakers limited to temperature or agitation control alone, the XC3-35RH employs a dual-loop PID-controlled refrigeration-heating system coupled with a high-fidelity orbital drive mechanism to simultaneously regulate chamber temperature (4–60 °C), relative humidity (optional R-series configuration), and oscillation parameters (30–300 rpm, 26 mm orbit). Its orbital motion—generated via brushless DC motor technology—ensures laminar fluid mixing with minimal shear stress, making it suitable for sensitive cell suspensions, microbial fermentations, hybridization assays, and enzyme kinetics studies where reproducible mass transfer and thermal homogeneity are critical. The unit’s modular stackable architecture supports up to three independently controlled layers, enabling scalable throughput without compromising individual chamber integrity—a design aligned with GLP-compliant multi-experiment workflows in academic, pharmaceutical, and biotech laboratories.

Key Features

• Triple-layer stackable configuration with independent temperature, humidity (R-series), and oscillation control per layer—eliminates cross-contamination risk and enables parallel condition screening.
• Brushless DC motor drive ensures consistent torque delivery across full speed range (30–300 rpm), zero maintenance, low electromagnetic interference, and <0.5% speed deviation under load.
• Full-color capacitive touchscreen interface with intuitive PID parameter navigation, real-time graphing of temperature/rotation/time, and multi-step programmable protocols (up to 10 segments per program).
• Integrated refrigeration system (R-series only) using environmentally compliant R134a compressor with adaptive defrost cycle and automatic ambient compensation for stable sub-ambient operation down to 4 °C.
• Electropolished 304 stainless steel inner chamber and reinforced tempered glass observation window with LED illumination—resistant to condensation, corrosion, and repeated sterilization cycles.
• Comprehensive safety suite: hardware-based over-temperature cutoff (dual-sensor redundancy), mechanical over-speed limiter, door-open interruption logic, power-fail recovery with last-state memory, and encrypted parameter lockout.

Sample Compatibility & Compliance

The XC3-35RH accommodates standard microbiological and cell culture vessels including Erlenmeyer flasks (250–3000 mL), deep-well plates, and custom bioreactor sleeves via standardized 520 × 420 mm stainless steel platforms per layer. Its uniform temperature distribution (±1 °C at setpoint) and calibrated humidity control (if equipped) comply with ASTM E2927-22 (Standard Practice for Performance Verification of Environmental Chambers) and support method validation under ISO/IEC 17025:2017. While not FDA 21 CFR Part 11–certified out-of-the-box, audit-ready data logging—including timestamped temperature, RPM, humidity, and alarm events—is exportable in CSV format for integration into validated LIMS or ELN systems. All electrical components meet IEC 61010-1 safety standards for laboratory equipment.

Software & Data Management

The embedded controller firmware supports USB data export (no proprietary software required) and includes built-in event logging with 30-day circular buffer retention. Critical parameters—including actual vs. setpoint deviations, cumulative runtime, and fault codes—are timestamped and stored with microsecond resolution. Optional RS485 Modbus RTU interface enables integration into centralized building management systems (BMS) or SCADA networks for remote monitoring and alarm forwarding. Firmware updates are performed via FAT32-formatted USB drive, with version rollback capability and SHA-256 checksum verification to ensure integrity.

Applications

• Microbial growth profiling under variable temperature/humidity gradients (e.g., E. coli, S. cerevisiae, filamentous fungi)
• Suspension culture of mammalian cells (CHO, HEK293) requiring low-shear orbital agitation and CO₂-independent pH stability
• Antibiotic susceptibility testing (AST) and time-kill curve analysis per CLSI M07-A11 guidelines
• Enzyme immobilization and protein refolding assays demanding precise thermal ramping and mixing synchronization
• Environmental simulation studies in ecotoxicology (e.g., algal growth under climate-relevant T/RH combinations)
• Stability testing of biologics and mRNA-LNP formulations per ICH Q5C recommendations

FAQ

Does the XC3-35RH support humidity control on all models?

Only R-series variants (e.g., XC3-35RH) include integrated humidification and dehumidification modules; non-R models provide temperature + agitation only.
Can individual layers operate at different temperatures simultaneously?

Yes—each of the three stackable layers features independent PID controllers, thermistors, and heating/cooling elements, allowing concurrent operation from 4 °C to 60 °C across layers.
Is the shaker compatible with sterile workflow requirements?

The stainless steel chamber, seamless corners, and autoclavable tray inserts meet ISO 14644-1 Class 5 cleanroom compatibility when used with validated surface disinfection protocols.
What is the maximum flask size supported per layer?

Standard configuration supports up to four 2000 mL or eight 3000 mL flasks per layer using optional reinforced support brackets.
Does the unit include validation documentation?

Factory-installed IQ/OQ documentation (per ASTM E2500-22) is available upon request; PQ execution requires user-defined test protocols aligned with internal SOPs.