DRETOP ZYC-312L Vertical Dual-Door Constant-Temperature Orbital Shaker

Overview

The DRETOP ZYC-312L is a vertically oriented, dual-door constant-temperature orbital shaker engineered for high-reproducibility cultivation and biochemical incubation under controlled thermal and mechanical conditions. It integrates the functional principles of an incubator and an orbital shaker within a single robust architecture—utilizing air-bath heating (no water jacket) for uniform temperature distribution across dual independent shaking platforms. The unit operates on orbital motion (26 mm diameter), which generates gentle yet effective mixing ideal for aerobic microbial growth, suspension cell culture, enzyme kinetics assays, and hybridization protocols where shear stress must be minimized. Its RT + 5°C to 60°C operational range accommodates standard mesophilic applications—including E. coli expression, yeast fermentation, mammalian cell passaging, and molecular biology workflows requiring stable thermal environments. Designed for GLP-compliant laboratories, the ZYC-312L supports traceable operation through embedded time-stamped logging and meets fundamental requirements for ISO/IEC 17025 environmental monitoring infrastructure.

Key Features

• Two independent stainless-steel shaking platforms (950 × 550 mm each), enabling simultaneous but separately configured experiments—e.g., one platform at 30°C/180 rpm for bacterial growth, another at 37°C/220 rpm for mammalian transfection.
• PID-controlled brushless DC motor delivering ±1 rpm speed stability and 0.1°C temperature resolution; real-time feedback ensures minimal deviation during extended runs (up to 999 hours).
• Large tempered glass double-door front access with integrated LED lighting—facilitating non-intrusive visual monitoring without compromising chamber integrity or thermal equilibrium.
• Electrostatically coated steel exterior and 304 stainless-steel interior chamber and tray surfaces—resistant to corrosion, compatible with common lab disinfectants, and compliant with cleaning validation protocols.
• Three-dimensional eccentric cam drive system with soft-start acceleration profile—eliminates liquid splashing during startup and maintains sample integrity across flask volumes from 50 mL to 5000 mL.
• Full parameter encryption and memory retention: settings persist through power interruption and restore automatically upon reboot—critical for unattended overnight or weekend operations.
• Comprehensive safety suite including over-temperature cutoff, over-speed shutdown, door-open interlock, and audible/visual alarms—aligned with IEC 61010-1 safety standards for laboratory equipment.

Sample Compatibility & Compliance

The ZYC-312L accommodates a wide range of vessel formats via interchangeable clamping systems: universal spring clamps (standard), conical flask holders (50–5000 mL), test tube racks (13–25 mm diameter), and custom adapters for irregular containers. Its dual-platform design allows heterogeneous loading—for instance, Erlenmeyer flasks on one shelf and deep-well microplates on the other—without cross-contamination risk. The optional O₂ enrichment port enables controlled aeration for high-density aerobic cultures, while the UV-C lamp module (254 nm) provides periodic decontamination of internal air and surfaces—supporting ISO 14644-1 Class 8 cleanroom adjunct use. Regulatory alignment includes conformity with CE marking directives (2014/30/EU EMC, 2014/35/EU LVD), and firmware architecture supports audit trails required under FDA 21 CFR Part 11 when paired with validated USB data export procedures.

Software & Data Management

Equipped with an embedded 4.3-inch backlit LCD interface, the ZYC-312L delivers intuitive navigation via tilt-optimized keypad and real-time visualization of setpoints, actual values (temperature, rpm, elapsed time), and system status. All operational parameters—including ramp rates, hold durations, and alarm thresholds—are programmable and stored in non-volatile memory. USB 2.0 interface enables direct download of time-series datasets (CSV format) containing timestamped temperature, speed, and runtime logs—enabling retrospective analysis in Excel, MATLAB, or LIMS-integrated platforms. No proprietary software is required; raw files are human-readable and compatible with automated QA/QC reporting pipelines. Optional firmware updates maintain compatibility with evolving laboratory informatics standards.

Applications

• Molecular Biology: Plasmid amplification, PCR master mix equilibration, restriction digestion incubations.
• Microbiology: Batch cultivation of Bacillus, Pseudomonas, and recombinant S. cerevisiae; antibiotic susceptibility testing under dynamic aeration.
• Cell Culture: Suspension CHO or HEK293 maintenance prior to transfection; serum-free adaptation protocols.
• Environmental Testing: BOD5 assays, biodegradation kinetics, activated sludge acclimation studies.
• Pharmaceutical R&D: Solubility screening, crystallization induction, excipient compatibility trials under agitation.

FAQ

Is the ZYC-312L suitable for sterile applications?
Yes—when equipped with the optional UV sterilization module and operated with validated cleaning SOPs, it supports aseptic technique for low-risk upstream processes.
Can both shelves operate at different temperatures?
No—the unit features a single air-bath chamber; both platforms share the same set temperature, though speed and timing may be independently controlled.
What is the maximum flask size supported per shelf?
Each shelf accommodates up to eight 2000 mL flasks or four 5000 mL flasks using reinforced clamps; load distribution must remain balanced to maintain orbital symmetry.
Does the device comply with FDA 21 CFR Part 11?
The hardware and USB logging functionality support Part 11 compliance when deployed within a validated environment—including user access controls, electronic signatures, and audit trail generation via third-party LIMS integration.
Is refrigeration available on this model?
No—ZYC-312L is a heating-only air-bath system. For sub-ambient operation, consider the ZYC-312LR variant with compressor-based cooling (4–60°C range).