Easysep-1050 Semi-Preparative High-Pressure Solvent Delivery Pump by Unimicro Technologies
| Brand | Unimicro Technologies |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | OEM Manufacturer |
| Product Category | Domestic |
| Model | Easysep-1050 |
| Instrument Type | Binary Pump |
| Flow Mode | Dual-Piston Reciprocating in Series |
| Flow Rate Range | 0.001–9.999 mL/min |
| Flow Resolution | 0.001 mL/min |
| Max Operating Pressure | 42 MPa (pressure upper/lower limit programmable with auto-alarm) |
| Flow Accuracy | ≤ ±0.3% |
| Flow Precision | ≤ 0.075% RSD |
| Pressure Pulsation | ≤ 0.1 MPa |
Overview
The Easysep-1050 Semi-Preparative High-Pressure Solvent Delivery Pump is an engineered solution for precise, stable, and scalable liquid delivery in preparative and semi-preparative chromatographic workflows. Designed and manufactured by Unimicro Technologies in Shanghai, this binary high-pressure pump operates on a dual-piston reciprocating-in-series architecture—optimized to deliver consistent flow under elevated backpressures encountered in packed-bed columns ranging from 10 mm to 50 mm internal diameter. Its core hydraulic design leverages Couette-flow principles within the pump head to minimize pulsation-induced band broadening, ensuring retention time stability critical for fraction collection and method transfer between analytical and preparative scales. The system supports gradient elution with two independently controlled solvent channels, enabling robust method development for complex natural product isolation, pharmaceutical impurity purification, and synthetic intermediate refinement.
Key Features
- Floating plunger mounting mechanism ensures dynamic concentricity between plunger rod and seal, significantly extending service life of both components under continuous high-pressure operation.
- Short-stroke plunger actuated by low-inertia cam reduces mechanical dwell and inertial overshoot, contributing to pulse suppression below 0.1 MPa (measured at 1 mL/min, 10 MPa, using a 10 µL compressibility-compensated test loop).
- Microprocessor-controlled microstepping drive circuit enables smooth, low-noise stepper motor operation—acoustic emission <45 dB(A) at 1 m distance during continuous flow.
- Embedded system-on-chip (SoC) architecture supports daisy-chain communication via a single RS-485 or Ethernet interface, allowing synchronized control of up to eight Easysep-1050 units within a single chromatography network.
- Real-time pressure and flow diagnostics with configurable alarm thresholds—including over-pressure, under-flow, prime failure, and seal leak detection—integrated into hardware-level firmware for fail-safe shutdown.
Sample Compatibility & Compliance
The Easysep-1050 is compatible with common HPLC and preparative LC solvents (e.g., water, methanol, acetonitrile, THF, ethyl acetate, and chlorinated hydrocarbons), provided chemical compatibility with stainless-steel 316L wetted parts and sapphire-plunger seals is verified. It meets structural integrity requirements per ISO 13485:2016 for medical device manufacturing environments and supports audit-ready operation under GLP and GMP frameworks. While not certified as FDA 21 CFR Part 11 compliant out-of-the-box, its embedded logging engine records timestamped operational events (start/stop, pressure excursions, error codes) with write-protection—enabling traceability when integrated with validated third-party CDS platforms.
Software & Data Management
Control is implemented through Unimicro’s proprietary EasyControl v3.x software suite (Windows 10/11, 64-bit), offering intuitive gradient programming, real-time pressure/flow waveform visualization, and method archiving with SHA-256 checksum verification. Raw diagnostic logs are stored in CSV format with millisecond-resolution timestamps; all configuration changes are logged with user ID and IP address when deployed in networked mode. Remote monitoring via Modbus TCP or OPC UA is supported for integration into MES or SCADA systems. Firmware updates are delivered via signed binary packages with cryptographic signature validation to prevent unauthorized modification.
Applications
- Semi-preparative purification of APIs and chiral compounds on 10–30 mm ID columns at flow rates up to 9.999 mL/min.
- Method scouting and scale-up studies bridging analytical UHPLC (e.g., 2.1 mm ID) to preparative systems (20+ mm ID).
- Automated fraction collection workflows requiring precise gradient reproducibility across multi-day runs.
- Quality control laboratories performing compendial testing per USP , EP 2.2.46, and JP 6.07 for dissolution and extract analysis.
- Academic and contract research labs conducting natural product dereplication where solvent flexibility and long-term baseline stability are essential.
FAQ
What is the maximum recommended column backpressure for continuous operation?
The Easysep-1050 is rated for continuous operation up to 42 MPa; however, sustained use above 35 MPa is advised only with active cooling of the pump head and verified seal integrity at elevated temperatures.
Can the pump be used in isocratic mode with a single solvent channel?
Yes—either channel A or B may be disabled in software; the inactive channel remains hydraulically isolated and does not contribute to baseline drift or cross-contamination.
Is flow calibration traceable to NIST standards?
Factory calibration is performed using gravimetric measurement against NIST-traceable mass standards (OIML R111 Class E2 weights) and certified temperature-controlled volumetric flasks; certificate of calibration is supplied with each unit.
Does the system support external triggering for fraction collectors?
Yes—TTL-compatible digital I/O ports provide programmable start/stop and pulse-per-mL signals, synchronized to flow accumulation with ±10 ms jitter.
What maintenance intervals are recommended for optimal performance?
Plunger seals should be inspected every 500 hours of operation or after 200 L of solvent throughput; pump head flushing is recommended weekly when using buffer-containing mobile phases.
