Elite P230A/P Analytical-to-Semi-Preparative Integrated HPLC System
| Brand | Elite |
|---|---|
| Origin | Liaoning, China |
| Manufacturer Type | Direct Manufacturer |
| Regional Category | Domestic (China) |
| Model | P230A/P |
| Application Level | Laboratory Grade |
| Instrument Type | High-Pressure Semi-Preparative Liquid Chromatography |
| Pump Flow Range | 0.01–40.00 mL/min (0.01 mL/min increment) |
| Flow Accuracy | ≤ ±1.0% |
| Flow Precision (RSD) | ≤ 0.2% |
| Maximum Operating Pressure | 30 MPa |
| Power Supply | AC 220 V ±10%, 50 Hz / 200 W |
| Dimensions (L×W×H) | 420 × 280 × 175 mm |
Overview
The Elite P230A/P Analytical-to-Semi-Preparative Integrated HPLC System is an engineered solution for laboratories requiring flexible, space-efficient chromatographic workflows across both analytical characterization and milligram-scale purification tasks. Based on high-pressure liquid chromatography (HPLC) principles—specifically utilizing isocratic or gradient elution under controlled flow and pressure—the system integrates a dual-role architecture: it operates as a high-resolution analytical HPLC platform for method development, identity confirmation, and purity assessment, and seamlessly transitions to a semi-preparative configuration for compound isolation and fraction collection. This functional duality eliminates the need for separate analytical and preparative instruments, reducing capital expenditure, lab footprint, and method transfer complexity. The system adheres to core chromatographic performance criteria defined in ISO 13877 (Liquid Chromatography — Determination of System Performance), with pump accuracy and precision validated per ASTM E260 and USP guidelines for chromatographic system suitability.
Key Features
- Modular dual-mode design enabling rapid reconfiguration between analytical and semi-preparative operation without hardware replacement—only valve positioning and column/detector cell selection are required.
- P230p high-pressure constant-flow pump featuring proprietary piston seal architecture and active pulse dampening; delivers stable flow from 0.01 to 40.00 mL/min with ≤ ±1.0% accuracy and ≤ 0.2% RSD over extended runtime.
- UV230II UV-Vis absorbance detector equipped with interchangeable flow cells: standard 10-mm pathlength cell for analytical work and semi-preparative cell (e.g., 6-mm pathlength, 1.5-mL volume) optimized for higher sample loading and reduced backpressure.
- Integrated Rheodyne-style manual injection valve and high-pressure switching valve (≥30 MPa rating) supporting automated fraction collection, column bypass, and solvent stream routing.
- Rugged mechanical construction with aluminum alloy chassis and thermally stabilized electronics compartment ensuring long-term operational stability in ambient laboratory environments (15–30 °C, <80% RH).
- Compliance-ready architecture: supports audit trail generation, user access levels, and electronic signature protocols aligned with FDA 21 CFR Part 11 requirements when paired with validated third-party chromatography data systems (CDS).
Sample Compatibility & Compliance
The P230A/P system accommodates a broad range of sample types—including small organic molecules, natural product extracts, synthetic intermediates, peptides, and polar/non-polar pharmaceutical actives—when used with reversed-phase (C18, C8), normal-phase (silica), or HILIC columns (2–21.2 mm i.d., up to 250 mm length). It supports mobile phases compatible with standard HPLC solvents (acetonitrile, methanol, water, buffers pH 2–8) and is fully compatible with post-column derivatization setups. Regulatory alignment includes conformance with ISO/IEC 17025 general requirements for testing laboratories, GLP documentation support via external CDS, and mechanical safety compliance per IEC 61010-1 for laboratory equipment. All wetted parts (pump heads, valves, tubing) are constructed from chemically resistant stainless steel 316 and PEEK, minimizing leaching and ensuring compatibility with aggressive solvents such as THF and chlorinated hydrocarbons.
Software & Data Management
While the P230A/P operates as a standalone instrument with front-panel control and real-time LED status indicators, it is designed for integration with industry-standard chromatography data systems (CDS) via RS-232 or optional USB-to-serial interface. Compatible platforms include Chromeleon™ (Thermo Fisher), Empower™ (Waters), and OpenLab CDS (Agilent). When connected, the system enables full remote control, sequence-based method execution, real-time chromatogram visualization, peak integration using AIA-compliant algorithms, and automated report generation compliant with ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) data integrity principles. Audit trails record all parameter changes, run starts/stops, and user logins—essential for GMP and regulatory submissions.
Applications
- Method development and transfer from analytical to semi-preparative scale using retention time and selectivity preservation strategies.
- Purification of synthetic reaction mixtures for NMR characterization or biological screening (typical load: 1–50 mg per injection).
- Isolation of bioactive compounds from plant extracts or fermentation broths prior to structural elucidation.
- Stability-indicating assays for pharmaceutical formulations under ICH Q1/Q5 conditions.
- Quality control of chiral intermediates using polysaccharide-based chiral columns at semi-preparative flow rates.
- Supporting academic and contract research organizations (CROs) in early-stage drug discovery where throughput, cost-efficiency, and scalability are critical.
FAQ
Can the P230A/P be used for fully automated preparative runs?
Yes—when integrated with a fraction collector and validated CDS software, the system supports timed or peak-triggered fraction collection with programmable valve switching.
What is the maximum column backpressure the system can sustain during semi-preparative operation?
The P230p pump and fluidic path are rated for continuous operation up to 30 MPa (4350 psi), accommodating sub-5 µm particles in columns up to 21.2 mm i.d.
Is the UV230II detector compliant with pharmacopeial wavelength accuracy requirements?
The detector employs a deuterium lamp with holographic grating and is factory-calibrated against NIST-traceable holmium oxide filters, meeting USP and EP 2.2.25 specifications for wavelength verification.
Does Elite provide IQ/OQ documentation packages?
Yes—qualified installation and operational qualification templates are available upon request and may be executed by certified field service engineers or qualified internal QA personnel.
Can the system be upgraded to include a binary gradient pump or autosampler?
The P230A/P platform is not designed for retrofitting with gradient modules or autosamplers; however, Elite offers the complementary P230-II series for advanced gradient applications and the eClass series for fully automated workflows.
