Eppendorf Research Plus Single-Channel Adjustable-Volume Pipette, 0.5–10 µL

Overview

The Eppendorf Research Plus Single-Channel Adjustable-Volume Pipette (0.5–10 µL) is a precision-engineered manual liquid handling instrument designed for high-reproducibility dispensing in molecular biology, clinical diagnostics, and pharmaceutical R&D laboratories. Built upon Eppendorf’s decades-long heritage in pipette innovation, this model employs an air displacement principle with a low-force piston mechanism and a fully sealed, corrosion-resistant internal system to ensure consistent aspiration and dispensing across its full volume range. Its ergonomic design—featuring a lightweight body, adjustable tip ejection force, and optimized center of gravity—reduces operator fatigue during extended use, supporting adherence to ISO/IEC 17025 and GLP-compliant workflows. The pipette is factory-calibrated according to ISO 8655-2 and traceable to national standards; optional UKAS-accredited calibration certificates are available for audit-ready documentation.

Key Features

• Ultra-low volume accuracy: Certified performance at ±0.04 µL (at 10 µL), meeting stringent requirements for qPCR, NGS library preparation, and enzyme kinetics assays.
• Positive-displacement-compatible architecture: Compatible with Eppendorf PhysioTip and LoRetention tips to minimize surface adsorption of viscous or low-volume samples (e.g., DNA, proteins, surfactants).
• One-handed volume adjustment: Dual-control dial allows precise, tactile volume setting without compromising grip stability or risking accidental changes.
• Chemical resistance: Housing constructed from high-grade polypropylene and stainless steel components resistant to common laboratory solvents including ethanol, isopropanol, and diluted acids/bases.
• Autoclavable upper body: Fully sterilizable at 121 °C / 20 min (EN 285) without disassembly—ensuring compliance with microbiology and cell culture applications requiring sterile technique.
• Integrated tip compatibility indicator: Visual color-coded ring (gray) confirms correct tip engagement and optimal sealing pressure for the 0.5–10 µL range.

Sample Compatibility & Compliance

The Research Plus 0.5–10 µL pipette demonstrates robust performance with aqueous buffers, glycerol-containing solutions (up to 40% v/v), and low-surface-tension liquids when used with appropriate low-retention tips. It complies with ISO 8655-2 (volumetric performance), ISO 8655-5 (calibration procedures), and DIN EN ISO 13485 (medical device quality management). For regulated environments—including FDA-regulated biomanufacturing and clinical labs—the pipette supports full traceability through optional electronic calibration logs and can be integrated into 21 CFR Part 11–compliant LIMS or ELN systems via third-party calibration management software. Routine verification per USP and CLSI EP15-A3 guidelines is facilitated by standardized gravimetric testing protocols.

Software & Data Management

While the manual Research Plus model does not incorporate onboard electronics, it is fully compatible with Eppendorf’s comprehensive digital ecosystem. Users may document calibration history, maintenance records, and usage logs using Eppendorf’s free LabLog software or integrate pipette data into enterprise platforms via CSV export. When paired with Eppendorf’s CertiCheck service, each unit receives a unique serial-numbered calibration report with uncertainty values, environmental conditions, and technician signature—supporting internal audits and regulatory inspections. All documentation adheres to ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).

Applications

This pipette is routinely deployed in critical low-volume applications where precision and repeatability are non-negotiable: quantitative real-time PCR setup (including master mix preparation at ≤2 µL volumes), CRISPR-Cas9 guide RNA dilution, single-cell lysate transfer, microfluidic device priming, and high-throughput screening assay plates (384-well and 1536-well formats). Its thermal stability ensures minimal volume drift between ambient (20–25 °C) and controlled cold-room environments (4–8 °C), making it suitable for cryogenic sample handling workflows. Laboratories performing ISO/IEC 17025-accredited testing routinely select this model for its documented metrological traceability and long-term stability (<0.5% volume drift over 12 months under standard use conditions).

FAQ

Is this pipette supplied with a calibration certificate?
Standard units ship with a factory calibration report; UKAS-accredited certificates are available upon request and require additional lead time.
Can I autoclave the entire pipette?
Only the upper body (piston chamber and shaft) is autoclavable at 121 °C for 20 minutes; the lower handle and tip cone must be cleaned with 70% ethanol and air-dried.
What tip types are recommended for 0.5–10 µL operation?
Eppendorf LoRetention Tips (Cat. No. 0030089102) and PhysioTips (Cat. No. 0030089110) are validated for optimal seal integrity and minimal carryover in this range.
Does Eppendorf offer service plans for routine recalibration?
Yes—Eppendorf ServicePlus contracts include annual on-site calibration, wear-part replacement, and performance verification aligned with ISO 8655-2 Annex D.
How does this model differ from the newer Eppendorf Xplorer series?
The Research Plus is a manually operated, mechanically calibrated platform optimized for cost-sensitive, high-precision routine work; the Xplorer series adds electronic volume control, programmable multi-step protocols, and Bluetooth connectivity for digital lab integration.