Eppendorf Research Plus Single-Channel Adjustable-Volume Pipette, 2–20 µL

Overview

The Eppendorf Research Plus Single-Channel Adjustable-Volume Pipette (2–20 µL) is an ergonomically engineered, precision liquid handling instrument designed for reproducible and traceable volumetric transfer in life science, clinical diagnostics, pharmaceutical quality control, and academic research laboratories. Based on the proven air displacement principle, this pipette delivers high accuracy and low variability across its full operating range through a patented spring-loaded tip cone mechanism that ensures consistent seal integrity with Eppendorf PhysioTip and standard universal pipette tips. Its all-metal shaft construction, combined with a lightweight yet rigid housing, minimizes operator fatigue during extended use while maintaining mechanical stability essential for repeatable aspiration and dispensing cycles. The Research Plus platform meets ISO 8655-2 (volumetric performance) and ISO 8655-5 (user-related requirements), supporting compliance with GLP, GMP, and FDA 21 CFR Part 11–aligned documentation workflows when integrated with validated software systems.

Key Features

• Adjustable volume setting via fine-tuned dial with tactile feedback and visual window indicating set volume in µL
• Ergonomic finger rest and balanced weight distribution reduce hand strain and improve control during repetitive pipetting
• Low-force plunger activation (≤2.5 N at maximum volume) enhances user comfort and minimizes risk of carpal tunnel syndrome
• Autoclavable upper body (121 °C, 20 min, 1 bar) without disassembly—ensures sterility and cross-contamination prevention
• Integrated tip ejection force limiter prevents accidental tip loss or sample splashing during ejection
• Calibration-friendly design with two-point adjustment (span and offset) accessible using standard calibration tools
• Chemical-resistant housing compatible with common laboratory solvents including 70% ethanol, isopropanol, and diluted acids/bases

Sample Compatibility & Compliance

This pipette is validated for use with aqueous solutions, buffers, cell suspensions, serum, and low-viscosity organic solvents. It is not recommended for highly volatile, corrosive, or viscous liquids (>100 mPa·s) without specialized tip adaptation or pre-wetting protocols. All Research Plus pipettes are manufactured under ISO 13485-certified quality management systems and conform to EN ISO 9001 standards. Performance validation data—including gravimetric accuracy, precision (CV ≤ 0.8% at 20 µL), and long-term repeatability—are provided in the Certificate of Conformance shipped with each unit. The device supports audit readiness for regulated environments: calibration records, maintenance logs, and user-defined operational parameters can be documented in accordance with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).

Software & Data Management

While the Research Plus 2–20 µL is a manual pipette and does not incorporate onboard electronics or Bluetooth connectivity, it is fully compatible with Eppendorf’s digital ecosystem. When used alongside the Eppendorf VisioNize Lab Suite or third-party LIMS platforms, manual calibration entries, usage logs, and preventive maintenance schedules can be centrally managed. Optional accessories—including the Eppendorf Calibration Software (v3.0+) and certified reference weights—enable automated calculation of deviation reports aligned with ISO/IEC 17025 requirements. Traceability is further enhanced through unique serial number registration and integration with Eppendorf’s Asset Management Portal, facilitating instrument lifecycle tracking from commissioning through decommissioning.

Applications

• Quantitative PCR (qPCR) setup requiring precise sub-microliter additions of master mix, primers, and template DNA
• High-throughput ELISA plate preparation with consistent reagent dispensing across 96- and 384-well formats
• Cell culture passaging where accurate dilution of trypsin-EDTA or media supplements is critical
• Standard curve generation in spectrophotometric assays (e.g., BCA, Bradford) demanding tight CVs below 1.0%
• Preparation of reference standards for LC-MS and GC-MS analysis under ISO/IEC 17025-accredited conditions
• Quality control testing in biopharmaceutical manufacturing per USP Analytical Instrument Qualification guidelines

FAQ

Is this pipette suitable for sterile applications?
Yes—the entire upper body (including plunger, shaft, and tip cone) is autoclavable at 121 °C for 20 minutes without disassembly. Tip cones retain dimensional stability after up to 100 autoclave cycles.
What calibration frequency is recommended?
Eppendorf recommends verification every 3 months for routine use; more frequent checks (e.g., daily or per shift) are advised in GLP/GMP environments or following mechanical shock or exposure to extreme temperatures.
Can I use non-Eppendorf tips with this pipette?
Yes—it accepts universal-fit pipette tips complying with ISO 8655-3 specifications. However, optimal accuracy and seal integrity are guaranteed only with Eppendorf PhysioTip or Reference 2.0 tips.
Does the pipette include a certificate of conformity?
Each unit ships with a factory-issued Certificate of Conformance listing serial number, date of manufacture, and gravimetric test results at 10 µL and 20 µL per ISO 8655-2.
How do I adjust the volume setting?
Rotate the volume dial clockwise or counterclockwise until the desired value appears in the clear display window. Always set volume within the specified range (2–20 µL); exceeding limits may damage internal mechanisms.