FlashDoctor-1000Pro Preparative Liquid Chromatography System by KEZHE Shanghai
| Brand | KEZHE Shanghai |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Domestic |
| Model | FlashDoctor-1000Pro |
| Application Level | Laboratory-Scale |
| Instrument Type | High-Pressure Preparative Liquid Chromatograph |
| Flow Rate Range | 0–200 mL/min |
| Flow Accuracy | ±1.5% |
| Pressure Rating | 200 psi (13.8 bar) |
| Wavelength Range | 190–850 nm |
| Wavelength Accuracy | ±1 nm |
| Spectral Bandwidth | 8 nm |
| Baseline Noise | ±0.2 mAU @ 254 nm |
| Baseline Drift | 0.2 mAU/30 min @ 254 nm |
| Flow Path Configuration | Binary Solvent Delivery |
| Fraction Collection | X-Y Robotic Matrix (135-tube capacity, standard 15 × 150 mm tubes) |
| Detection Options | UV/Vis Detector (D₂ + Tungsten lamp), optional ELSD |
| Column Compatibility | Normal-phase (e.g., silica, 12 g & 25 g), reversed-phase (C18, 40 g), and empty loading columns (12 g) |
| Software Features | Method-based fraction triggering, peak integration & quantitation, audit trail, user management, lamp life monitoring, intensity calibration, TLC-guided method recommendation |
Overview
The FlashDoctor-1000Pro Preparative Liquid Chromatography System is a compact, integrated high-pressure preparative LC platform engineered for laboratory-scale purification of organic compounds, natural products, pharmaceutical intermediates, and synthetic reaction mixtures. Built upon classical liquid chromatographic principles—employing gradient or isocratic elution with binary solvent delivery—the system delivers reproducible separation performance under controlled pressure conditions up to 200 psi (13.8 bar). Its design targets mid-throughput purification workflows where speed, operational simplicity, and method transferability from analytical to preparative scale are critical. The system complies with fundamental chromatographic practice standards referenced in pharmacopoeial methods—including Chinese National Standard GB 5009.202–2016 for polar compound analysis in edible oils—and supports method development aligned with ICH Q2(R2) guidelines for analytical procedure validation.
Key Features
- Binary high-pressure pump module with flow range 0–200 mL/min and accuracy of ±1.5%, featuring real-time pressure monitoring, overpressure alarm, and automatic shutdown to safeguard column integrity and system stability.
- Dual-lamp UV/Vis detector (deuterium + tungsten) covering 190–850 nm; wavelength accuracy ±1 nm, spectral bandwidth 8 nm, and low-noise optical path (±0.2 mAU baseline noise at 254 nm).
- X-Y robotic fraction collector with 135-position capacity for standard 15 × 150 mm test tubes; supports full-collection, peak-triggered, time-window, and manual collection modes.
- Integrated touchscreen control interface with workflow-driven software architecture—enabling one-click fraction collection, automatic peak detection, and real-time chromatogram display.
- Modular column rack accommodating multiple column formats: two normal-phase silica columns (12 g and 25 g), one reversed-phase C18 column (40 g), and one empty loading column (12 g) for sample preloading.
- Solvent level sensing and bubble detection circuitry prevent air ingress during extended runs, ensuring uninterrupted pump operation and consistent flow delivery.
- Reversible flow-path configuration allows rapid switching between normal-phase and reversed-phase modes without hardware reconfiguration.
- Optional ELSD module available for universal, non-UV-absorbing analyte detection—particularly valuable for carbohydrates, lipids, glycopeptides, and other low-UV-response compounds.
Sample Compatibility & Compliance
The FlashDoctor-1000Pro accommodates diverse sample matrices including crude reaction mixtures, plant extracts, fermentation broths, and synthetic intermediates. Its column compatibility spans silica, amino, cyano, diol, and C18 stationary phases—supporting both adsorption and partition mechanisms. The system meets essential regulatory expectations for laboratory instrumentation: data integrity is maintained via software-enforced audit trail (aligned with FDA 21 CFR Part 11 principles), role-based user access control, electronic signature support, and timestamped method/version logging. While not certified for GMP production environments, it fulfills GLP-aligned documentation requirements for method development, impurity profiling, and reference standard isolation in R&D laboratories.
Software & Data Management
The embedded chromatography data system (CDS) provides comprehensive acquisition, processing, and reporting capabilities. It includes automated peak integration with customizable baseline algorithms, retention time alignment across runs, and quantitative analysis using external/internal standard calibration. All instrument parameters, method files, chromatograms, and fraction logs are stored in a structured local database with export options to CSV, PDF, and AIA (.cdf) formats. Software features include lamp usage tracking with predictive maintenance alerts, photometric intensity calibration routines, and TLC-based method recommendation—where Rf values input manually guide initial solvent strength selection and gradient slope estimation. Data security protocols enforce password-protected user profiles and session timeouts, satisfying institutional IT policies for instrument network deployment.
Applications
- Purification of active pharmaceutical ingredients (APIs) and synthetic analogs during medicinal chemistry campaigns.
- Isolation of bioactive natural product fractions from botanical extracts for structure elucidation and biological screening.
- Removal of catalysts, ligands, and unreacted starting materials following cross-coupling or asymmetric synthesis.
- Preparative separation of isomers (e.g., regioisomers, diastereomers) under optimized chiral or achiral conditions.
- Method scouting and scale-up studies bridging analytical HPLC and pilot-scale purification.
- Compliance-driven isolation of polar components in food matrices per GB 5009.202–2016 and analogous AOAC or ISO protocols.
FAQ
What is the maximum operating pressure of the FlashDoctor-1000Pro?
The system is rated for continuous operation up to 200 psi (13.8 bar), suitable for flash chromatography and low-to-moderate pressure preparative separations.
Can the UV detector be used for quantitative analysis?
Yes—the detector supports linear dynamic range calibration and peak area integration with configurable integration parameters, enabling reliable quantitation across validated concentration ranges.
Is method transfer from analytical HPLC possible?
Method transfer is supported through retention time normalization and scalable flow/column dimension adjustments; the software includes scaling calculators for gradient time and volume translation.
Does the system support 21 CFR Part 11 compliance?
It implements core Part 11 elements—including electronic signatures, audit trail, and user access control—but requires local validation documentation and procedural controls to achieve full compliance in regulated environments.
What column formats are compatible beyond the included set?
The column manifold accepts standard 1/4″ and 1/8″ stainless steel or PEEK fittings; users may install custom-packed or commercial cartridges ranging from 4 g to 120 g, provided mechanical and pressure limits are observed.
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