Genscript AmMag™ Quatro Automated Plasmid Extraction System
| Brand | Genscript |
|---|---|
| Origin | Jiangsu, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | AmMag™ Quatro |
| Price Range | USD 70,000 – 140,000 |
Overview
The Genscript AmMag™ Quatro Automated Plasmid Extraction System is an integrated benchtop platform engineered for high-throughput, mid-scale isolation of high-purity plasmid DNA from bacterial cultures. It implements a magnetic bead–based solid-phase extraction workflow, eliminating reliance on centrifugation at all critical steps—including cell lysis clarification, nucleic acid binding, wash cycles, and elution. This architecture enables fully automated processing of up to 24 samples (each derived from up to 1.5 g wet bacterial pellet) in approximately 2.5 hours. The system is designed to deliver plasmid yields and purity profiles comparable to traditional anion-exchange column–based methods—while significantly reducing hands-on time, inter-operator variability, and risk of cross-contamination. Its operational principle centers on controlled magnetic separation under programmable liquid handling, precise temperature-regulated incubation (for lysis and neutralization), and optimized buffer exchange kinetics—ensuring consistent recovery of supercoiled isoforms essential for transfection, viral packaging, and therapeutic vector production.
Key Features
- Fully automated 24-sample processing with walk-away operation and minimal user intervention
- Magnetic bead–driven workflow: no centrifugation required during binding, washing, or elution steps
- Integrated thermal control modules for standardized lysis (alkaline/SDS), neutralization, and optional RNase A digestion
- Pre-validated, kit-compatible reagent system—including Endofree™-grade kits (e.g., L01031-24) for low-endotoxin applications
- Configurable elution volumes (50–1000 µL) to balance concentration requirements against total yield needs
- Onboard UV-Vis spectrophotometer integration support for post-extraction QC (OD260/280, OD260/230) via compatible third-party modules
- Robust architecture compliant with ISO 13485–aligned manufacturing practices and CE IVD marking for in vitro diagnostic use
Sample Compatibility & Compliance
The AmMag™ Quatro supports standard E. coli strains (e.g., DH5α, TOP10, BL21) grown in LB, TB, or SOB media. It accommodates input biomass ranging from 0.5 g to 1.5 g wet pellet per sample, with scalability validated across common plasmid backbones (pUC, pET, pCDNA, lentiviral, AAV). All supported kits—including L01031-24 (Endofree Plasmid Purification Kit) and L00882-24 (Standard Plasmid Purification Kit)—are manufactured under GMP-like quality controls and tested for endotoxin levels ≤100 EU/mg. The system meets requirements for GLP-compliant workflows when paired with audit-trail–enabled software (see Software section) and supports documentation traceability aligned with FDA 21 CFR Part 11 principles. Validation reports are available upon request for ISO/IEC 17025–accredited laboratories.
Software & Data Management
The AmMag™ Quatro operates via Genscript’s proprietary QuatroControl™ software, offering intuitive protocol selection, real-time status monitoring, and step-by-step run logging. Each extraction cycle generates a timestamped digital log file containing instrument parameters (temperature setpoints, magnet engagement timing, pipetting volumes, dwell times), reagent lot numbers (when scanned), and operator ID. Optional data export supports CSV and PDF formats for integration into LIMS environments. For regulated environments, the software can be configured with role-based access control, electronic signatures, and full audit trail functionality—meeting foundational expectations for 21 CFR Part 11 compliance when deployed on validated IT infrastructure.
Applications
- Production of research-grade and preclinical plasmid DNA for transient transfection and stable cell line generation
- High-fidelity plasmid preparation for viral vector manufacturing (lentivirus, AAV, retrovirus)
- Endotoxin-sensitive applications including primary cell transfection, immunotherapy development, and in vivo delivery studies
- QC release testing of plasmid batches prior to downstream enzymatic modification or sequencing
- Scalable nucleic acid purification in core facilities supporting CRISPR library construction or synthetic biology pipelines
FAQ
What is the typical yield and purity profile when using the AmMag™ Quatro with L00882-24?
Yields range from 500–600 µg per 1.5 g E. coli pellet, with OD260/280 ratios consistently between 1.85–1.95 and OD260/230 >2.10. Supercoiled content exceeds 90% as confirmed by agarose gel electrophoresis.
Can the system process Endofree-grade plasmids suitable for in vivo use?
Yes—when used with L01031-24 kits and following recommended protocols, endotoxin levels are routinely ≤100 EU/mg, meeting specifications for most non-clinical in vivo applications.
Is method validation support available for regulated labs?
Genscript provides IQ/OQ documentation templates, performance qualification test scripts, and reagent traceability records to support internal validation efforts in GxP environments.
How does the AmMag™ Quatro compare to manual column-based purification in terms of reproducibility?
Inter-run CVs for yield and purity metrics are typically <8%, reflecting improved consistency over manual methods where operator-dependent variables introduce higher variability.
Does the system support custom protocol development?
Yes—QuatroControl™ allows advanced users to define custom liquid handling sequences, incubation profiles, and magnet timing parameters, subject to hardware safety limits.
