GreenPrima TH-7001 Calcium & Magnesium Hardness Reagent Kit for Colorimetric Analysis

Overview

The GreenPrima TH-7001 Calcium & Magnesium Hardness Reagent Kit is a precision-formulated reagent system engineered exclusively for use with the Aqualysis 800 online water quality analyzer. It enables fully automated, photometric endpoint detection of total water hardness—expressed as calcium carbonate (CaCO₃)—via standardized titration-based colorimetry. This method relies on the selective chelation of Ca²⁺ and Mg²⁺ ions by ethylenediaminetetraacetic acid (EDTA) under buffered alkaline conditions, with endpoint determination achieved through absorbance measurement at 570 nm using an integrated dual-beam photometer. Unlike single-point colorimetric strips or manual titrations, the TH-7001 reagent set ensures stoichiometric consistency, minimized interferences from transition metals, and reproducible endpoint discrimination—critical for regulatory-grade process monitoring in GMP-compliant pharmaceutical water systems, boiler feedwater circuits, and closed-loop cooling applications.

Key Features

• Optimized for Aqualysis 800 platform: Pre-calibrated for seamless integration with instrument’s auto-titration sequence and optical path calibration routines
• Narrow-range formulation (0.534–5.34 ppm as CaCO₃): Delivers enhanced resolution and lower detection limits compared to broad-spectrum reagents, ideal for ultrapure water verification
• Stabilized indicator chemistry: Uses Eriochrome Black T analog with proprietary pH buffering (pH 10.0 ± 0.1) to suppress interference from Fe³⁺, Al³⁺, and Mn²⁺ within specified limits (Fe < 3 ppm, Al < 0.1 ppm, Mn < 0.2 ppm)
• Low reagent consumption: Each kit supports ≥ 3,000 analyses under standard 3-minute cycle time, reducing operational cost and waste generation
• Shelf-stable liquid reagents: Supplied in amber HDPE bottles with inert headspace; unopened stability ≥ 24 months at 10–30°C; post-opening stability ≥ 6 months when stored at 15–25°C
• Traceable lot documentation: Each batch includes CoA (Certificate of Analysis) with verified EDTA molarity, indicator extinction coefficient, and blank absorbance specifications

Sample Compatibility & Compliance

The TH-7001 reagent system is validated for use with representative water matrices meeting the Aqualysis 800’s inlet specifications: pH 4–10, temperature 5–40°C, pressure 0.2–6 bar (recommended 1–2 bar), and defined metal ion thresholds. It is routinely deployed in applications requiring adherence to pharmacopeial standards—including USP (Water for Injection hardness limit ≤ 0.1 ppm), EP 2.2.24 (Purified Water), and JP 17 (Sterile Water for Injection). In industrial settings, it supports ASTM D1126 Class I (low-hardness boiler feedwater) and ISO 6059 Category A (drinking and process water) testing protocols. The reagent formulation excludes mercury-based indicators and complies with REACH Annex XVII restrictions on hazardous substances.

Software & Data Management

When used with the Aqualysis 800 analyzer, TH-7001 measurements are fully integrated into the instrument’s embedded firmware and optional Aqualysis Connect software suite. All analysis events—including reagent lot ID, calibration timestamp, raw absorbance values, endpoint volume, and calculated hardness—are timestamped and stored locally (≥ 10,000 records) with audit-trail functionality compliant with FDA 21 CFR Part 11 (electronic signatures, operator login, change history). Data export supports CSV via USB or real-time transmission via RS485 Modbus RTU or 4–20 mA analog output. Optional cloud synchronization enables remote validation review and trend analysis across multi-site GMP facilities.

Applications

• Pharmaceutical water systems: Continuous hardness monitoring of purified water (PW), water for injection (WFI), and clean steam condensate
• Power generation: Boiler feedwater quality assurance and demineralizer effluent verification
• Industrial cooling: Real-time control of softener regeneration cycles in HVAC and manufacturing cooling towers
• Food & beverage: Incoming municipal water qualification and RO permeate hardness validation
• Municipal pretreatment: Low-level hardness screening prior to advanced oxidation or membrane filtration
• Research laboratories: Reference-standard preparation and method validation for EPA Method 130.1 (Hardness by EDTA Titration)

FAQ

Is TH-7001 compatible with other analyzers besides Aqualysis 800?
No—TH-7001 is chemically and volumetrically optimized for the Aqualysis 800’s specific pump stroke calibration, photometric cell geometry, and titration algorithm. Cross-platform use may result in non-linear response or endpoint misidentification.
What is the shelf life after opening the reagent bottles?
Unrefrigerated storage at 15–25°C maintains performance for up to 6 months post-opening. Avoid repeated exposure to ambient humidity and direct sunlight.
Does TH-7001 require daily recalibration?
No—systematic calibration is performed during initial setup and after each reagent kit replacement. Drift verification is automatically conducted every 24 hours using factory-certified zero and span standards.
Can TH-7001 detect hardness contributions from ions other than Ca²⁺ and Mg²⁺?
The method is highly selective for Ca²⁺ and Mg²⁺ under controlled pH and masking conditions. Trace contributions from Sr²⁺ or Ba²⁺ are theoretically possible but negligible (<0.5% relative error) within the stated operating range and matrix constraints.
How does TH-7001 support GLP/GMP documentation requirements?
Each kit includes a unique lot number traceable to manufacturing batch records, CoA with metrological traceability to NIST SRM 1692a (Calcium Carbonate Standard), and full compatibility with Aqualysis 800’s 21 CFR Part 11-compliant electronic record system.