Hanbon NS4330 Tri-Solvent Preparative HPLC Pump System

Overview

The Hanbon NS4330 Tri-Solvent Preparative HPLC Pump System is a high-pressure, laboratory-scale preparative liquid chromatography platform engineered for robust gradient elution, scalable purification, and reproducible fraction collection in synthetic chemistry, natural product isolation, and pharmaceutical process development. Built upon dual NP7030C high-pressure solvent delivery modules, the system implements active high-pressure mixing via a dynamic mixer to achieve precise ternary gradient formation—enabling seamless transition between three mobile phase solvents without flow interruption. Its core architecture follows classical reciprocating piston pump design with sapphire-coated plunger seals and ceramic check valves, ensuring long-term stability under sustained 15 MPa pressure operation. The system operates within ISO/IEC 17025-aligned laboratory environments and supports GLP-compliant workflows through integrated stroke-count logging and audit-ready event timestamps.

Key Features

• Dual NP7030C high-pressure pumps (300 mL/min head capacity each), configured for parallel or series operation to support flow rates from 0.1 to 3000.0 mL/min with ±1.5% accuracy and ≤1% RSD flow precision
• Real-time, pump-running gradient reprogramming—flow rate and composition can be modified online without pausing the separation
• Integrated stroke counter for each plunger assembly, compliant with GLP documentation requirements for maintenance scheduling and lifetime tracking
• Dynamic axial compression (DAC) compatibility via DAC-HB80 column interface, supporting column diameters up to 80 mm and enabling uniform bed packing under variable load conditions
• Modular expandability: optional NU3000C UV-Vis detector (0.5 nm wavelength resolution, 0.1 nm reproducibility), INJ-P manual preparative injection valve with 20 mL loop, and network controller for remote monitoring
• Stainless-steel fluidic path with inline 2 µm filter, rated for organic/aqueous mobile phases including THF, DCM, and high-salt buffers

Sample Compatibility & Compliance

The NS4330 accommodates a broad range of sample matrices—including small-molecule APIs, peptides, oligonucleotides, and plant extracts—with compatibility across reversed-phase (C18, C8), silica, amino, and ion-exchange preparative columns. All wetted components meet USP Class VI biocompatibility standards. The system conforms to ASTM E2656-21 for preparative LC performance verification and supports IQ/OQ protocols per GMP Annex 15. Audit trail functionality—including user login, method change history, and pump stroke logs—is implemented in EasyChrom-1000 software to satisfy FDA 21 CFR Part 11 requirements for electronic records and signatures.

Software & Data Management

EasyChrom-1000 chromatography workstation provides full system control, real-time chromatogram visualization, peak integration, and fraction triggering logic. It supports method templating, sequence batch processing, and automated report generation in PDF/CSV formats. Raw data files are stored in vendor-neutral .chd format with embedded metadata (instrument ID, timestamp, operator, method parameters). The software includes built-in calibration tools for flow verification (gravimetric or volumetric) and wavelength accuracy checks using holmium oxide reference filters. Network controller integration enables centralized deployment across multi-user lab networks with role-based access control.

Applications

• Purification of reaction mixtures in medicinal chemistry (e.g., removal of Pd catalysts, protecting group cleavage byproducts)
• Isolation of bioactive compounds from crude botanical extracts at gram-to-100g scale
• Preparative chiral separations using polysaccharide-based columns (Chiralpak®, Chiralcel®)
• Process validation support for early-phase API manufacturing, including impurity profiling and forced degradation studies
• Method scouting and transfer from analytical to preparative scale using retention time normalization algorithms

FAQ

Does the NS4330 support gradient methods with three solvents simultaneously?
Yes—the dual-pump configuration with dynamic mixer enables true high-pressure ternary gradient formation; all three solvents are mixed pre-column at pressures up to 15 MPa.
Can the system operate under GMP-compliant conditions?
Yes—when deployed with EasyChrom-1000 in audit-trail mode and validated IQ/OQ documentation, it meets baseline requirements for Phase I–II process development labs under ICH Q5A and Q7 guidelines.
What is the maximum column diameter supported with the DAC-HB80 interface?
The DAC-HB80 column is rated for internal diameters up to 80 mm and operating pressures up to 12 MPa during compression cycles.
Is remote monitoring and control possible?
Yes—the embedded network controller supports TCP/IP communication, allowing browser-based status monitoring and limited parameter adjustment via secure HTTPS interface.
How is flow accuracy verified during routine qualification?
Users may perform gravimetric flow verification per ASTM E2656-21 using certified balance and timer, with results automatically logged in the system’s calibration history module.