Hanuo HN-08 Stomacher-Type Aseptic Homogenizer
| Brand | Hanuo |
|---|---|
| Origin | Shanghai, China |
| Equipment Type | Stomacher-Type (Paddle-Beating) |
| Model | HN-08 |
| Capacity Range | 3–400 mL |
| Dimensions (W×D×H) | 380 × 220 × 330 mm |
| Weight | 22 kg |
| Power Supply | 220 V / 50 Hz |
| Operating Temperature | 0–35 °C |
| Programmable Timer | 1 s – 100 min (3 user-defined presets) |
| Beating Frequency | 3–12 cycles/sec (3 programmable speed profiles) |
| Adjustable Beating Gap | 0–50 mm |
| Sterile Bag Size | 17 × 30 cm |
| Includes | 1 pack of sterile filter bags (10 pcs) |
| Optional Accessory | Sample rack |
Overview
The Hanuo HN-08 Stomacher-Type Aseptic Homogenizer is an engineered solution for gentle, reproducible mechanical homogenization of biological and food samples under controlled aseptic conditions. It operates on the stomacher principle—applying rhythmic, bidirectional compression and shearing forces via two opposing paddles against a sealed sterile sample bag—enabling efficient release of microorganisms from solid matrices without thermal denaturation, cellular lysis artifacts, or cross-contamination. Unlike rotor-stator or ultrasonic systems, this method preserves microbial viability and structural integrity of fragile cells (e.g., probiotics, spores, or Gram-negative pathogens), making it especially suitable for ISO 6887-1, ISO 7218, FDA-BAM, and USP / compliant microbiological testing workflows in QC laboratories, contract testing facilities, and academic research settings.
Key Features
- Gentle yet effective homogenization: Optimized paddle geometry and adjustable beating frequency (3–12 cycles/sec) ensure uniform dispersion while minimizing heat generation (<1.5 °C rise over 2-min cycle at 10 Hz).
- Aseptic workflow integration: Fully enclosed stainless-steel chamber with transparent polycarbonate viewing window and full-swing front door enables rapid bag loading/unloading without exposure to ambient air.
- Programmable operation: Three independent memory slots store time/speed/gap configurations; timer resolution down to 1 second supports precise protocol replication across shifts.
- Ergonomic design: Adjustable beating gap (0–50 mm) accommodates varying bag fill volumes and viscosity profiles—critical for heterogeneous samples such as minced meat, leafy greens, or powdered pharmaceuticals.
- Low-maintenance architecture: No internal contact with sample material; only the disposable sterile bag interfaces with the specimen—eliminating cleaning validation, autoclaving downtime, or carryover risk.
- Compliance-ready interface: Backlit LCD display with English-language menu navigation supports GLP documentation requirements; all parameter changes are timestamped in non-volatile memory.
Sample Compatibility & Compliance
The HN-08 accepts standard 17 × 30 cm sterile filter bags (included), compatible with common commercial brands (e.g., Seward, Interscience, Thermo Fisher). It handles viscous, particulate-laden, and low-water-activity matrices—including raw poultry, fermented dairy, herbal extracts, cosmetic emulsions, and lyophilized APIs—without clogging or bag rupture when operated within recommended volume (3–400 mL) and time limits. The device meets CE marking requirements for laboratory equipment (2014/30/EU EMC Directive and 2014/35/EU LVD), and its operational parameters align with ISO/IEC 17025-accredited methods for sample preparation prior to plate count, MPN, or molecular detection (qPCR, NGS library prep). Routine use satisfies audit criteria for FDA 21 CFR Part 11 data integrity when paired with validated electronic logbook software.
Software & Data Management
While the HN-08 operates as a standalone instrument with embedded firmware, its programmable memory and real-time parameter display support traceable manual recordkeeping per ISO 15197 and CLSI EP22-A. For integrated lab informatics, optional RS-232 or USB-to-serial adapters enable connection to LIMS or ELN platforms—capturing start/stop timestamps, selected program ID, actual run duration, and final speed setting. All configuration changes generate immutable logs stored onboard for ≥12 months, fulfilling GMP Annex 11 and EU GMP Chapter 4 data retention expectations.
Applications
- Food safety labs: Enumeration of Salmonella, Listeria monocytogenes, and E. coli O157:H7 from meat, produce, and ready-to-eat meals per ISO 6579-1 and EN ISO 11290.
- Pharmaceutical QC: Recovery of bioburden from non-sterile drug products, excipients, and packaging components per USP and Ph. Eur. 2.6.12.
- Clinical & environmental microbiology: Homogenization of swab eluates, biofilm-coated surfaces, and soil suspensions for culture-independent analysis.
- Academic research: Preparation of intact tissue homogenates for flow cytometry, RNA extraction, or metabolite profiling where enzymatic or thermal degradation must be avoided.
FAQ
Is the HN-08 compatible with third-party sterile bags?
Yes—it accepts industry-standard 17 × 30 cm filter bags with polyethylene or nylon mesh (pore size ≥100 µm); compatibility should be verified using manufacturer-provided dimensional tolerances.
Does the unit require calibration or periodic verification?
No formal calibration is mandated, but users should perform quarterly performance checks using reference samples (e.g., standardized Enterococcus faecalis suspension) to confirm recovery consistency per ISO/IEC 17025 clause 7.7.
Can the HN-08 be used for mycoplasma or viral sample processing?
It is not validated for viral titer recovery or mycoplasma release due to insufficient shear force for capsid disruption; consult ISO 18593 for alternative homogenization strategies.
What maintenance is required beyond routine cleaning?
Only external wipe-down with 70% ethanol; no lubrication, sensor recalibration, or internal servicing is necessary under normal operating conditions.
Is there a service manual or technical support available in English?
Hanuo provides bilingual (EN/CN) operation manuals and firmware update packages upon registration; technical inquiries are supported via email with ≤24-h response time during business hours (GMT+8).
