Hanuo HNX-1500 Microplate Orbital Shaker

Overview

The Hanuo HNX-1500 Microplate Orbital Shaker is an engineered solution for precise, reproducible mixing of samples in standard microtiter plates used across ELISA, cell-based assays, immunoassays, and high-throughput screening workflows. Designed around orbital shaking mechanics—where the platform rotates in a circular path with a fixed 3 mm diameter—the instrument generates uniform, low-shear agitation ideal for suspension of adherent cells, antigen-antibody binding enhancement, and reagent homogenization without splashing or cross-contamination. Unlike vortex or linear shakers, orbital motion ensures consistent kinetic energy distribution across all wells, minimizing edge effects and supporting assay robustness. Its brushless DC motor delivers stable rotational output across the full 300–1500 rpm range, with <±2% speed deviation under nominal load conditions—critical for protocol standardization in regulated environments.

Key Features

• Four-position microplate platform accommodating up to four standard 96-well or 384-well plates simultaneously—optimized for lab throughput without compromising mechanical stability.
• High-resolution LED digital display showing real-time setpoints and remaining time; intuitive membrane keypad enables rapid parameter entry and recall.
• Microprocessor-controlled timing circuitry with programmable duration from 1 minute to 99 hours 59 minutes, supporting both single-step and multi-stage incubation-mixing protocols.
• Brushless DC motor architecture ensures >20,000-hour service life, zero carbon dust generation, and silent operation (<45 dB(A) at 1 m), suitable for shared lab spaces and cleanroom-adjacent workflows.
• Orbital motion profile calibrated to ISO 8573-1 Class 5 air purity compatibility—no internal lubricants or volatile outgassing, preserving sample integrity in sensitive biochemical applications.
• Integrated audible alarm signals program completion; optional external relay output (via accessory port) supports integration with LIMS or environmental monitoring systems.

Sample Compatibility & Compliance

The HNX-1500 is validated for use with ANSI/SLAS-standard microplates (including skirted, semi-skirted, and unskirted formats), PCR plates, and deep-well blocks up to 2.2 mL well volume. It maintains plate flatness within ±0.15 mm across the entire platform surface under maximum load—ensuring reliable seal integrity for sealed assay kits. The device complies with IEC 61010-1:2010 (Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use) and carries CE marking per EU Directive 2014/30/EU (EMC) and 2014/35/EU (LVD). While not a GLP/GMP-certified instrument per se, its deterministic timer logic, non-volatile parameter memory, and auditable end-of-cycle notification support traceability requirements in ISO 17025-accredited testing laboratories.

Software & Data Management

The HNX-1500 operates as a standalone benchtop unit with no embedded software stack or network interface. All operational parameters—including speed, time, and alarm status—are retained in non-volatile memory after power loss. For labs requiring electronic recordkeeping, the device supports manual logging via timestamped entries in laboratory notebooks or integration into broader workflow systems using its TTL-compatible external trigger port (sold separately). No proprietary drivers or cloud services are required; configuration remains fully accessible through front-panel controls without firmware updates or license dependencies.

Applications

• ELISA plate washing and conjugate incubation steps where consistent agitation improves signal-to-noise ratios.
• Cell dissociation and resuspension prior to flow cytometry or viability staining.
• Antibody labeling reactions requiring gentle yet thorough mixing over extended durations.
• Enzyme kinetics assays involving multi-reagent additions and timed endpoint readings.
• Pre-analytical sample preparation for mass spectrometry or NGS library construction workflows.
• Quality control testing of diagnostic kits under ISO 13485-aligned manufacturing protocols.

FAQ

Is the HNX-1500 compatible with 384-well plates?
Yes—it accommodates ANSI/SLAS-compliant 384-well microplates when loaded individually or in pairs per platform quadrant.
Does it support temperature control?
No—the HNX-1500 is a non-incubating shaker; it must be operated inside temperature-stabilized enclosures if thermal regulation is required.
Can the oscillation amplitude be adjusted?
No—the 3 mm orbital diameter is mechanically fixed to ensure repeatability and avoid calibration drift across user sessions.
What maintenance is required?
None beyond periodic surface cleaning with 70% ethanol; the brushless motor eliminates bearing lubrication or belt replacement needs.
Is there a warranty or service support available internationally?
Hanuo provides a 24-month limited warranty on parts and labor; technical documentation and spare parts (e.g., fuse, power cord, PCB assembly) are distributed globally via authorized channel partners.