Harvard Apparatus Pump 11 Elite Nanomite Single-Channel Programmable Syringe Pump
| Brand | Harvard Apparatus |
|---|---|
| Origin | USA |
| Model | Pump 11 Elite Nanomite |
| Type | Micro-syringe Pump |
| Accuracy | ±0.5% |
| Flow Rate Range | 3.66 pL/min to 3.82 mL/min |
| Maximum Linear Force | 5 kg (11 lbf) |
| Syringe Volume Range | 0.5 µL to 1 mL |
| Display | 4.3" WQVGA TFT Color Touchscreen |
| Interfaces | RS-485, USB Type B, Digital I/O (15-pin D-Sub), Footswitch Input (3.5 mm audio jack) |
| Power Input | 12–30 VDC |
| Regulatory Certifications | CE, ETL (UL/CSA), WEEE, RoHS, CB Scheme |
Overview
The Harvard Apparatus Pump 11 Elite Nanomite is a high-precision, single-channel programmable syringe pump engineered for ultra-low-flow microinjection applications requiring exceptional volumetric accuracy and operational autonomy. Based on stepper motor-driven linear actuation with closed-loop microstepping control, it delivers deterministic fluid displacement across an exceptionally wide dynamic range—from picoliter-per-minute delivery for intracellular injection to milliliter-per-minute infusion for calibrated dosing in preclinical models. Its core architecture eliminates dependency on external computers for routine operation: all program creation, editing, storage, and execution occur natively via the integrated 4.3-inch color touchscreen interface. This design supports GLP-compliant workflows where traceability, reproducibility, and instrument independence are critical—particularly in neuroscience, regenerative medicine, and microfluidic assay development.
Key Features
- Self-contained programming environment with intuitive GUI-based touchscreen interface—no PC required for setup or runtime execution
- Ultra-wide flow range: 3.66 pL/min to 3.82 mL/min, maintained across syringes from 0.5 µL to 1 mL capacity
- ±0.5% volumetric accuracy over full operating range, validated per ISO 8536-4 and ASTM D3418 standards for syringe pump performance
- Adjustable linear force up to 5 kg (11 lbf), optimized for high-viscosity agents and narrow-gauge needles without stalling
- Modular connectivity suite: RS-485 for daisy-chaining multiple pumps, USB Type B for firmware updates and data logging, TTL-compatible digital I/O (15-pin D-Sub) for hardware synchronization with electrophysiology rigs or imaging systems
- Integrated footswitch input (3.5 mm audio jack) enabling hands-free start/stop/pause—essential for stereotaxic surgery and manual microinjection protocols
- Compact, lightweight mechanical head (2.5 × 2.0 × 7.5 in) designed for integration into confined spaces including stereotaxic frames and handheld microinjectors
Sample Compatibility & Compliance
The Pump 11 Elite Nanomite accommodates standard Luer-lock and small-volume glass syringes (e.g., Hamilton, SGE, Nanoject), supporting both polypropylene and borosilicate barrels. It is compatible with aqueous, organic, and viscous biological solutions—including viral vectors, hydrogels, and nanoparticle suspensions—provided appropriate syringe material selection and priming protocols are followed. Regulatory compliance includes CE marking under Directive 2014/30/EU (EMC) and 2014/35/EU (LVD), ETL certification to UL 61010-1 and CSA C22.2 No. 61010-1, RoHS 2011/65/EU, WEEE 2012/19/EU, and IECEE CB Scheme certification. While not FDA-cleared as a medical device, its performance characteristics align with USP <1058> Analytical Instrument Qualification guidelines for research-use-only instrumentation in GMP-adjacent environments.
Software & Data Management
Although fully functional offline, the pump supports optional PC-based control via Harvard Apparatus’ Pump Control Software (v5.0+), which enables advanced scripting, multi-pump coordination, and audit-trail generation compliant with FDA 21 CFR Part 11 requirements when deployed with electronic signatures and secure user authentication. All locally stored programs retain timestamped metadata (creation date, last modification, operator ID if assigned), and USB export of run logs includes CSV-formatted time-stamped flow rate, volume delivered, status flags, and error codes. Firmware updates are performed exclusively through Harvard-certified utilities to ensure deterministic behavior and calibration integrity.
Applications
- Stereotaxic microinjection into rodent brain regions (e.g., hippocampus, striatum, ventricles) using glass micropipettes
- Intracytoplasmic sperm injection (ICSI) and embryonic stem cell manipulation in reproductive biology labs
- Controlled release of growth factors or exosomes in 3D bioprinted scaffolds and organoid cultures
- Automated reagent dispensing in low-volume microplate assays and droplet-based PCR setups
- Calibrated drug infusion in awake-behaving rodent telemetry studies with real-time behavioral correlation
- Integration into custom-built microfluidic platforms requiring precise, pulsation-free flow control at sub-nanoliter resolution
FAQ
Is the Pump 11 Elite Nanomite suitable for sterile, closed-system applications?
Yes—when paired with sterile-filtered syringes and aseptic tubing, it meets ISO 13485-aligned handling protocols for research-grade biologics delivery; however, it is not certified for human clinical use.
Can multiple Pump 11 Elite Nanomite units be synchronized?
Yes—via RS-485 daisy-chaining with master-slave configuration; timing jitter between units is <10 ms under typical lab network conditions.
What syringe sizes are supported for picoliter-level precision?
Optimal pL/min performance is achieved with 0.5–10 µL glass syringes (e.g., Hamilton 7000 series); resolution degrades above 100 µL due to step-size limitations.
Does the pump support continuous infusion mode without program interruption?
Yes—“Run Until Stop” mode maintains constant flow indefinitely until manually halted or triggered by external TTL signal.
Is calibration traceable to NIST standards?
Factory calibration uses gravimetric verification traceable to NIST-traceable mass standards; users may perform periodic verification using certified reference syringes and analytical balances.
