HengAo HAS-100B Portable Air Microbial Sampler

Overview

The HengAo HAS-100B Portable Air Microbial Sampler is a compact, battery-powered active air sampler engineered for quantitative collection of viable airborne microorganisms—including bacteria, fungi, and yeasts—via inertial impaction onto standard 90 mm agar plates. Designed in alignment with the principles outlined in GB/T 18883–2002 (China’s Indoor Air Quality Standard) and consistent with internationally recognized impactor-based methodologies—including adaptations of the Andersen cascade principle—the HAS-100B delivers reproducible, culture-based enumeration of colony-forming units (CFUs) per cubic meter of air. Its fixed-flow architecture ensures consistent volumetric sampling across variable environmental conditions, supporting routine environmental monitoring (EM) programs in regulated pharmaceutical cleanrooms, hospital isolation wards, biosafety laboratories, and public health field deployments. Unlike passive settle plates or filtration-based systems, the HAS-100B provides controlled, time-resolved microbial load data essential for trend analysis, contamination source tracing, and ISO 14644-1/ISO 14698-1 compliance verification.

Key Features

• Stable 100 L/min constant-flow sampling, calibrated to ±1% accuracy using an integrated thermal mass flow sensor
• Rotatable Petri dish stage with adjustable rotational speed to ensure uniform microbial deposition across the agar surface
• Detachable stainless-steel sampling head compatible with autoclaving at 121 °C for repeated sterilization without cross-contamination risk
• Dot-matrix LCD interface with real-time display of elapsed time, remaining battery capacity, and flow status
• Rechargeable Ni-MH battery pack enabling up to 4 hours of continuous operation (typical usage); low-battery indicator alerts prior to shutdown
• Intelligent power management: backlight deactivates after 30 seconds of inactivity; unit enters sleep mode after 5 minutes in standby
• Ergonomic handheld design with optional telescoping tripod mount (maximum sampling height: 1.5 m), suitable for both indoor and outdoor ambient air assessment
• Robust mechanical construction rated for operation across −30 °C to +40 °C and 20–80% RH, ensuring reliability in diverse climatic and facility environments

Sample Compatibility & Compliance

The HAS-100B accepts standard 90 mm Petri dishes filled with selective or non-selective growth media (e.g., Tryptic Soy Agar, Sabouraud Dextrose Agar, or malt extract agar), enabling cultivation and identification of mesophilic bacteria, molds, and yeasts. Its impactor geometry and flow profile are optimized for efficient capture of particles ≥0.65 µm aerodynamic diameter—covering the majority of clinically and industrially relevant bioaerosols. While not certified to ISO 14698-2 for validation documentation out-of-the-box, the device supports user qualification protocols (IQ/OQ/PQ) and is routinely deployed in facilities operating under GMP (Annex 1), GLP, and ISO 13485 frameworks. Data generated may be incorporated into environmental monitoring reports aligned with USP , EU GMP Annex 1, and WHO TRS 970 requirements.

Software & Data Management

The HAS-100B operates as a standalone hardware platform without embedded Bluetooth or USB connectivity. All operational parameters—including start/stop time, total sampling duration, and flow confirmation—are logged locally via the LCD interface and manually recorded by the operator. For traceability in regulated environments, users are advised to integrate sampling events into electronic laboratory notebooks (ELNs) or LIMS platforms using standardized templates. Optional external logging accessories (e.g., time-stamped digital cameras, calibrated timers) may be employed to satisfy audit requirements for 21 CFR Part 11-compliant workflows, particularly where electronic records of sampling metadata are mandated.

Applications

• Routine environmental monitoring in Grade A–D cleanrooms of pharmaceutical manufacturing facilities
• Post-sanitization verification and aseptic process simulation (media fill) support
• Outbreak investigation and pathogen surveillance in clinical settings (e.g., operating theaters, ICU air handling units)
• Baseline microbial profiling of ambient air in municipal water treatment plants, food processing zones, and research greenhouses
• Educational microbiology labs requiring portable, hands-on aerosol sampling instrumentation
• Field-based public health assessments during seasonal fungal spore events or post-flood mold investigations

FAQ

Is the HAS-100B validated for ISO 14644-1 classification studies?
The HAS-100B is suitable for routine EM but is not pre-validated for formal cleanroom classification per ISO 14644-1; users must perform their own flow calibration, recovery efficiency testing, and uncertainty budgeting as part of qualification.
Can the sampling head be sterilized in an autoclave?
Yes—the stainless-steel impactor head is fully detachable and rated for repeated autoclaving at 121 °C, 15 psi for 15–20 minutes.
Does the device support external power supply during extended sampling?
No—HAS-100B operates exclusively on its internal Ni-MH battery; no DC input port or AC adapter is provided.
What is the recommended maintenance interval?
Daily visual inspection of the nozzle and agar plate alignment is advised; full cleaning and flow recalibration should occur every 100 operating hours or per facility SOP.
Is firmware upgrade capability available?
No—the HAS-100B uses fixed-function embedded logic with no field-upgradable firmware or software modules.