HengAo HSE-12B Manual Solid Phase Extraction System

Overview

The HengAo HSE-12B is a manually operated, pressure-driven solid phase extraction (SPE) system engineered for reproducible, high-throughput sample preparation in analytical laboratories. It operates on the principle of selective adsorption and elution using disposable SPE cartridges packed with sorbents such as C18, silica, Florisil®, or ion-exchange resins. By applying regulated positive pressure—typically via an external nitrogen or compressed air source—the system drives sample solution and solvents through the cartridge bed at controlled flow rates (1–30 mL/min), enabling precise retention of target analytes and efficient removal of matrix interferences. Unlike vacuum-based SPE manifolds, the HSE-12B’s pressurized architecture ensures consistent flow dynamics across all 12 channels, minimizing channel-to-channel variability and improving method robustness—particularly critical for trace-level analysis in regulated environments.

Key Features

• 12-port parallel manifold design with modular port spacing, accommodating standard 1 mL, 3 mL, 6 mL, and 12 mL SPE cartridges without adapter kits
• PTFE-wetted fluid path ensures chemical inertness and compatibility with aggressive solvents (e.g., dichloromethane, acetonitrile, concentrated acids/bases)
• Integrated pressure regulator with dual-stage filtration and built-in pressure gauge (0–100 psi range) for stable, fine-tuned flow control
• Modular construction: interchangeable base plates support optional 24-position configurations and round-format manifolds for lab-specific workflow adaptation
• Stainless steel frame with corrosion-resistant surface finish and borosilicate glass collection tubes (graduated, 10–60 mL capacity) for visual monitoring and solvent recovery
• No electrical components required—designed for use in fume hoods, cleanrooms, or mobile field labs where spark-free operation is essential

Sample Compatibility & Compliance

The HSE-12B supports diverse sample matrices including aqueous environmental extracts (drinking water, wastewater, leachates), biological fluids (serum, plasma, urine, tissue homogenates), food homogenates (fruits, vegetables, meat, dairy), and complex industrial effluents. Its PTFE-sealed interface prevents cross-contamination and enables compliance with method requirements under EPA Method 505, 525.3, 8270, ISO 17993:2022 (water quality — determination of organic compounds by SPE-LC-MS), and AOAC Official Methods® for pesticide residue analysis. While the instrument itself is not certified to GLP or GMP standards, its mechanical design facilitates full traceability when integrated into validated workflows—including documented pressure settings, flow rate verification logs, and cartridge lot tracking per USP Analytical Instrument Qualification guidelines.

Software & Data Management

As a manual, non-electronic platform, the HSE-12B does not incorporate embedded software or digital interfaces. However, it is fully compatible with laboratory information management systems (LIMS) and electronic lab notebooks (ELN) through standardized procedural documentation. Users may record operational parameters—including applied pressure (psi), elapsed time per step, solvent volumes, and cartridge batch numbers—in structured templates aligned with 21 CFR Part 11 audit-trail requirements when paired with compliant ELN platforms (e.g., LabArchives, Benchling). The absence of firmware eliminates cybersecurity vulnerabilities and reduces qualification burden during IQ/OQ/PQ validation.

Applications

• Environmental Monitoring: Pre-concentration of trace pesticides (e.g., organophosphates, triazines), PAHs, PCBs, and metals-chelates from surface water, groundwater, and soil extracts prior to GC-MS or ICP-MS analysis
• Food Safety Testing: Cleanup of QuEChERS extracts from fruits, grains, and animal tissues; removal of co-extracted lipids, pigments, and organic acids prior to LC-MS/MS quantitation
• Pharmaceutical & Biomedical Research: Isolation of drugs and metabolites from serum, urine, or cell culture media; fractionation of protein-bound vs. free drug forms using mixed-mode sorbents
• Forensic Toxicology: Selective enrichment of opioids, benzodiazepines, and stimulants from postmortem blood or hair digests under chain-of-custody protocols
• Beverage Quality Control: Removal of polyphenols and organic acids from wine, juice, or beer samples to prevent column fouling in HPLC methods

FAQ

Is the HSE-12B compatible with 24-well microplate collection formats?
Yes—optional adapter plates are available to reconfigure the manifold for direct elution into 24-well deep-well or shallow-well microplates.
Can the system be used with corrosive solvents like HF or hot concentrated HNO₃?
No—while PTFE exhibits excellent resistance to most organics and dilute mineral acids, hydrofluoric acid and boiling nitric acid exceed its safe operating limits; consult material compatibility charts before use.
Does the unit include a pressure source?
No—users must supply a clean, oil-free nitrogen or compressed air source (regulated to ≤100 psi) with appropriate quick-connect fittings.
What is the recommended maintenance schedule?
Inspect O-rings and PTFE seals quarterly; replace annually or after 500 cycles. Clean stainless steel surfaces with isopropanol; rinse glassware with deionized water and dry in a particle-free environment.
Is technical support available in English for international customers?
Yes—HengAo provides English-language user manuals, application notes, and remote troubleshooting assistance via email and web-based ticketing system.