High-Throughput Bioreactor Shaker Dai-PH08

Overview

The Dai-PH08 High-Throughput Bioreactor Shaker is an integrated, temperature-controlled orbital shaker engineered for parallel cultivation of microbial, mammalian, or insect cell cultures in multiple glass bioreactors under tightly regulated physicochemical conditions. Unlike conventional incubator shakers, the Dai-PH08 incorporates a dual-loop PID control architecture that independently regulates chamber temperature and rotational dynamics while supporting real-time pH monitoring and automated acid/base feeding via dedicated peristaltic modules. Its core operating principle relies on orbital oscillation (Φ25 mm nominal amplitude, scalable to 300 mm) driven by a single-axis balanced motor assembly—ensuring low-vibration, high-reproducibility motion across all vessel positions. The unit maintains thermal stability between 4 °C and 60 °C with ±0.1 °C resolution and ±0.5 °C uniformity throughout the 880 × 550 × 785 mm internal chamber, enabled by forced-air convection and CFC-free R134a refrigeration. Designed for scalability in process development labs, it accommodates up to six high-borosilicate glass vessels (with standardized M12 threaded top ports for pH, DO, and sampling integration), making it suitable for strain screening, media optimization, and early-stage bioprocess characterization.

Key Features

• Single-platform orbital shaker with programmable rotation speed (40–300 rpm, ±1 rpm accuracy) and adjustable amplitude (Φ25 mm base; extended range up to 300 mm)
• Dual-zone PID temperature control with independent sensor feedback loops and forced-air circulation for uniform thermal distribution
• Integrated pH interface support: M12-threaded top ports compatible with standard industrial pH electrodes (e.g., Mettler Toledo InPro series)
• Onboard acid/base dosing capability (0.2–200 mL/min flow range) controlled via PLC-driven peristaltic pumps
• Standalone operation enabled by full-color capacitive touchscreen interface with embedded data logging (time-stamped temperature, RPM, pH setpoint, feed volume)
• Rugged mechanical construction: mirror-finish 316L stainless steel chamber interior; structural frame built from cold-rolled steel, angle iron, and channel sections
• Compliance-ready architecture: supports audit trails, user access levels, and electronic record retention aligned with FDA 21 CFR Part 11 principles

Sample Compatibility & Compliance

The Dai-PH08 accepts standard cylindrical bioreactor vessels fabricated from ASTM F799-compliant borosilicate glass (e.g., Schott DURAN® equivalents), with nominal volumes ranging from 500 mL to 5 L per vessel. All vessel tops feature M12 × 0.75 internal threads for secure mounting of pH probes, gas spargers, temperature sensors, and needle-based sampling/inoculation systems. The chamber design permits simultaneous operation of up to six vessels without cross-contamination risk. From a regulatory perspective, the system’s deterministic control logic, non-volatile parameter storage, and timestamped event logging meet foundational requirements for GLP and GMP environments. It is compatible with IQ/OQ/PQ qualification protocols per ASTM E2500 and supports documentation packages compliant with ISO 13485 Annex A for medical device-related bioprocess development.

Software & Data Management

No external PC is required for operation or data capture. The embedded microcontroller runs a deterministic real-time OS with a capacitive touchscreen GUI offering multi-language support (English, German, Chinese). All operational parameters—including setpoints, actual values, alarm states, and feed volumes—are logged at user-defined intervals (1 s to 60 min) into encrypted onboard flash memory (≥16 GB). Export options include USB mass-storage mode (CSV format) and optional Ethernet/Wi-Fi connectivity for integration into LIMS or MES platforms. Audit trail functionality records operator ID, timestamp, parameter change, and reason code for every critical action—fully traceable for regulatory review. Data integrity safeguards include write-protection modes, cyclic redundancy checks (CRC32), and automatic backup upon power loss.

Applications

• High-throughput microbial strain evaluation under varied pH and temperature regimes
• Media optimization studies requiring parallel pH-stat or chemostat-like control across ≥6 vessels
• Seed train expansion for CHO or HEK293 cultures with synchronized agitation and thermal conditioning
• Process analytical technology (PAT) development for DOE-based bioreactor scale-down models
• Academic and industrial bioprocess R&D where reproducible oxygen transfer (kLa), mixing time, and shear profiles must be maintained across replicates
• Regulatory filing support: generation of comparable kinetic datasets for comparability protocols (ICH Q5E)

FAQ

Does the Dai-PH08 support dissolved oxygen (DO) monitoring and control?
Yes—while DO sensing requires optional external polarographic or optical probes, the system provides dedicated analog input channels and relay outputs to integrate third-party DO controllers and gas-mixing manifolds.
Can the shaker accommodate non-standard vessel geometries?
The platform is optimized for cylindrical vessels with flat bottoms and M12 top ports. Custom clamping fixtures are available upon request for conical or flanged configurations.
Is remote monitoring possible without installing proprietary software?
Yes—the unit supports Modbus TCP and HTTP API endpoints for read-only access to live parameters and historical logs via standard web browsers or SCADA systems.
What validation documentation is supplied with the instrument?
Each unit ships with Factory Acceptance Test (FAT) reports, calibration certificates for temperature and RPM sensors (NIST-traceable), and a complete IQ/OQ protocol template compliant with ASTM E2500.
How is maintenance access designed for long-term reliability?
Front-access service panels allow full replacement of cooling fans, drive belts, and peristaltic pump heads without disassembling the structural frame or chamber liner.